Response of physiological parameters in Dionaea muscipula J. Ellis teratomas transformed with rolB oncogene

Abstract Background Plant transformation with rol oncogenes derived from wild strains of Rhizobium rhizogenes is a popular biotechnology tool. Transformation effects depend on the type of rol gene, expression level, and the number of gene copies incorporated into the plant’s genomic DNA. Although ro...

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Autores principales:	Wojciech Makowski, Aleksandra Królicka, Barbara Tokarz, Karolina Miernicka, Anna Kołton, Łukasz Pięta, Kamilla Malek, Halina Ekiert, Agnieszka Szopa, Krzysztof Michał Tokarz
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Rhizobium rhizogenes Primary and secondary metabolism Venus flytrap Rol genes Transformation Botany QK1-989
Acceso en línea:	https://doaj.org/article/cc56688a8b884675974de18c64e2c930
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spelling	oai:doaj.org-article:cc56688a8b884675974de18c64e2c9302021-12-05T12:06:22ZResponse of physiological parameters in Dionaea muscipula J. Ellis teratomas transformed with rolB oncogene10.1186/s12870-021-03320-y1471-2229https://doaj.org/article/cc56688a8b884675974de18c64e2c9302021-11-01T00:00:00Zhttps://doi.org/10.1186/s12870-021-03320-yhttps://doaj.org/toc/1471-2229Abstract Background Plant transformation with rol oncogenes derived from wild strains of Rhizobium rhizogenes is a popular biotechnology tool. Transformation effects depend on the type of rol gene, expression level, and the number of gene copies incorporated into the plant’s genomic DNA. Although rol oncogenes are known as inducers of plant secondary metabolism, little is known about the physiological response of plants subjected to transformation. Results In this study, the physiological consequences of rolB oncogene incorporation into the DNA of Dionaea muscipula J. Ellis was evaluated at the level of primary and secondary metabolism. Examination of the teratoma (transformed shoots) cultures of two different clones (K and L) showed two different strategies for dealing with the presence of the rolB gene. Clone K showed an increased ratio of free fatty acids to lipids, superoxide dismutase activity, synthesis of the oxidised form of glutathione, and total pool of glutathione and carotenoids, in comparison to non-transformed plants (control). Clone L was characterised by increased accumulation of malondialdehyde, proline, activity of superoxide dismutase and catalase, total pool of glutathione, ratio of reduced form of glutathione to oxidised form, and accumulation of selected phenolic acids. Moreover, clone L had an enhanced ratio of total triglycerides to lipids and accumulated saccharose, fructose, glucose, and tyrosine. Conclusions This study showed that plant transformation with the rolB oncogene derived from R. rhizogenes induces a pleiotropic effect in plant tissue after transformation. Examination of D. muscipula plant in the context of transformation with wild strains of R. rhizogenes can be a new source of knowledge about primary and secondary metabolites in transgenic organisms.Wojciech MakowskiAleksandra KrólickaBarbara TokarzKarolina MiernickaAnna KołtonŁukasz PiętaKamilla MalekHalina EkiertAgnieszka SzopaKrzysztof Michał TokarzBMCarticleRhizobium rhizogenesPrimary and secondary metabolismVenus flytrapRol genesTransformationBotanyQK1-989ENBMC Plant Biology, Vol 21, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Rhizobium rhizogenes Primary and secondary metabolism Venus flytrap Rol genes Transformation Botany QK1-989
spellingShingle	Rhizobium rhizogenes Primary and secondary metabolism Venus flytrap Rol genes Transformation Botany QK1-989 Wojciech Makowski Aleksandra Królicka Barbara Tokarz Karolina Miernicka Anna Kołton Łukasz Pięta Kamilla Malek Halina Ekiert Agnieszka Szopa Krzysztof Michał Tokarz Response of physiological parameters in Dionaea muscipula J. Ellis teratomas transformed with rolB oncogene
description	Abstract Background Plant transformation with rol oncogenes derived from wild strains of Rhizobium rhizogenes is a popular biotechnology tool. Transformation effects depend on the type of rol gene, expression level, and the number of gene copies incorporated into the plant’s genomic DNA. Although rol oncogenes are known as inducers of plant secondary metabolism, little is known about the physiological response of plants subjected to transformation. Results In this study, the physiological consequences of rolB oncogene incorporation into the DNA of Dionaea muscipula J. Ellis was evaluated at the level of primary and secondary metabolism. Examination of the teratoma (transformed shoots) cultures of two different clones (K and L) showed two different strategies for dealing with the presence of the rolB gene. Clone K showed an increased ratio of free fatty acids to lipids, superoxide dismutase activity, synthesis of the oxidised form of glutathione, and total pool of glutathione and carotenoids, in comparison to non-transformed plants (control). Clone L was characterised by increased accumulation of malondialdehyde, proline, activity of superoxide dismutase and catalase, total pool of glutathione, ratio of reduced form of glutathione to oxidised form, and accumulation of selected phenolic acids. Moreover, clone L had an enhanced ratio of total triglycerides to lipids and accumulated saccharose, fructose, glucose, and tyrosine. Conclusions This study showed that plant transformation with the rolB oncogene derived from R. rhizogenes induces a pleiotropic effect in plant tissue after transformation. Examination of D. muscipula plant in the context of transformation with wild strains of R. rhizogenes can be a new source of knowledge about primary and secondary metabolites in transgenic organisms.
format	article
author	Wojciech Makowski Aleksandra Królicka Barbara Tokarz Karolina Miernicka Anna Kołton Łukasz Pięta Kamilla Malek Halina Ekiert Agnieszka Szopa Krzysztof Michał Tokarz
author_facet	Wojciech Makowski Aleksandra Królicka Barbara Tokarz Karolina Miernicka Anna Kołton Łukasz Pięta Kamilla Malek Halina Ekiert Agnieszka Szopa Krzysztof Michał Tokarz
author_sort	Wojciech Makowski
title	Response of physiological parameters in Dionaea muscipula J. Ellis teratomas transformed with rolB oncogene
title_short	Response of physiological parameters in Dionaea muscipula J. Ellis teratomas transformed with rolB oncogene
title_full	Response of physiological parameters in Dionaea muscipula J. Ellis teratomas transformed with rolB oncogene
title_fullStr	Response of physiological parameters in Dionaea muscipula J. Ellis teratomas transformed with rolB oncogene
title_full_unstemmed	Response of physiological parameters in Dionaea muscipula J. Ellis teratomas transformed with rolB oncogene
title_sort	response of physiological parameters in dionaea muscipula j. ellis teratomas transformed with rolb oncogene
publisher	BMC
publishDate	2021
url	https://doaj.org/article/cc56688a8b884675974de18c64e2c930
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Response of physiological parameters in Dionaea muscipula J. Ellis teratomas transformed with rolB oncogene

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