Transcriptional progression during meiotic prophase I reveals sex-specific features and X chromosome dynamics in human fetal female germline.

During gametogenesis in mammals, meiosis ensures the production of haploid gametes. The timing and length of meiosis to produce female and male gametes differ considerably. In contrast to males, meiotic prophase I in females initiates during development. Hence, the knowledge regarding progression th...

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Autores principales:	Xueying Fan, Ioannis Moustakas, Vanessa Torrens-Juaneda, Qijing Lei, Geert Hamer, Leoni A Louwe, Gonneke S K Pilgram, Karoly Szuhai, Roberto Matorras, Cristina Eguizabal, Lucette van der Westerlaken, Hailiang Mei, Susana M Chuva de Sousa Lopes
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Genetics QH426-470
Acceso en línea:	https://doaj.org/article/cc6bb2ecaa424a22922367940bd877c2
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spelling	oai:doaj.org-article:cc6bb2ecaa424a22922367940bd877c22021-12-02T20:03:20ZTranscriptional progression during meiotic prophase I reveals sex-specific features and X chromosome dynamics in human fetal female germline.1553-73901553-740410.1371/journal.pgen.1009773https://doaj.org/article/cc6bb2ecaa424a22922367940bd877c22021-09-01T00:00:00Zhttps://doi.org/10.1371/journal.pgen.1009773https://doaj.org/toc/1553-7390https://doaj.org/toc/1553-7404During gametogenesis in mammals, meiosis ensures the production of haploid gametes. The timing and length of meiosis to produce female and male gametes differ considerably. In contrast to males, meiotic prophase I in females initiates during development. Hence, the knowledge regarding progression through meiotic prophase I is mainly focused on human male spermatogenesis and female oocyte maturation during adulthood. Therefore, it remains unclear how the different stages of meiotic prophase I between human oogenesis and spermatogenesis compare. Analysis of single-cell transcriptomics data from human fetal germ cells (FGC) allowed us to identify the molecular signatures of female meiotic prophase I stages leptotene, zygotene, pachytene and diplotene. We have compared those between male and female germ cells in similar stages of meiotic prophase I and revealed conserved and specific features between sexes. We identified not only key players involved in the process of meiosis, but also highlighted the molecular components that could be responsible for changes in cellular morphology that occur during this developmental period, when the female FGC acquire their typical (sex-specific) oocyte shape as well as sex-differences in the regulation of DNA methylation. Analysis of X-linked expression between sexes during meiotic prophase I suggested a transient X-linked enrichment during female pachytene, that contrasts with the meiotic sex chromosome inactivation in males. Our study of the events that take place during meiotic prophase I provide a better understanding not only of female meiosis during development, but also highlights biomarkers that can be used to study infertility and offers insights in germline sex dimorphism in humans.Xueying FanIoannis MoustakasVanessa Torrens-JuanedaQijing LeiGeert HamerLeoni A LouweGonneke S K PilgramKaroly SzuhaiRoberto MatorrasCristina EguizabalLucette van der WesterlakenHailiang MeiSusana M Chuva de Sousa LopesPublic Library of Science (PLoS)articleGeneticsQH426-470ENPLoS Genetics, Vol 17, Iss 9, p e1009773 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Genetics QH426-470
spellingShingle	Genetics QH426-470 Xueying Fan Ioannis Moustakas Vanessa Torrens-Juaneda Qijing Lei Geert Hamer Leoni A Louwe Gonneke S K Pilgram Karoly Szuhai Roberto Matorras Cristina Eguizabal Lucette van der Westerlaken Hailiang Mei Susana M Chuva de Sousa Lopes Transcriptional progression during meiotic prophase I reveals sex-specific features and X chromosome dynamics in human fetal female germline.
description	During gametogenesis in mammals, meiosis ensures the production of haploid gametes. The timing and length of meiosis to produce female and male gametes differ considerably. In contrast to males, meiotic prophase I in females initiates during development. Hence, the knowledge regarding progression through meiotic prophase I is mainly focused on human male spermatogenesis and female oocyte maturation during adulthood. Therefore, it remains unclear how the different stages of meiotic prophase I between human oogenesis and spermatogenesis compare. Analysis of single-cell transcriptomics data from human fetal germ cells (FGC) allowed us to identify the molecular signatures of female meiotic prophase I stages leptotene, zygotene, pachytene and diplotene. We have compared those between male and female germ cells in similar stages of meiotic prophase I and revealed conserved and specific features between sexes. We identified not only key players involved in the process of meiosis, but also highlighted the molecular components that could be responsible for changes in cellular morphology that occur during this developmental period, when the female FGC acquire their typical (sex-specific) oocyte shape as well as sex-differences in the regulation of DNA methylation. Analysis of X-linked expression between sexes during meiotic prophase I suggested a transient X-linked enrichment during female pachytene, that contrasts with the meiotic sex chromosome inactivation in males. Our study of the events that take place during meiotic prophase I provide a better understanding not only of female meiosis during development, but also highlights biomarkers that can be used to study infertility and offers insights in germline sex dimorphism in humans.
format	article
author	Xueying Fan Ioannis Moustakas Vanessa Torrens-Juaneda Qijing Lei Geert Hamer Leoni A Louwe Gonneke S K Pilgram Karoly Szuhai Roberto Matorras Cristina Eguizabal Lucette van der Westerlaken Hailiang Mei Susana M Chuva de Sousa Lopes
author_facet	Xueying Fan Ioannis Moustakas Vanessa Torrens-Juaneda Qijing Lei Geert Hamer Leoni A Louwe Gonneke S K Pilgram Karoly Szuhai Roberto Matorras Cristina Eguizabal Lucette van der Westerlaken Hailiang Mei Susana M Chuva de Sousa Lopes
author_sort	Xueying Fan
title	Transcriptional progression during meiotic prophase I reveals sex-specific features and X chromosome dynamics in human fetal female germline.
title_short	Transcriptional progression during meiotic prophase I reveals sex-specific features and X chromosome dynamics in human fetal female germline.
title_full	Transcriptional progression during meiotic prophase I reveals sex-specific features and X chromosome dynamics in human fetal female germline.
title_fullStr	Transcriptional progression during meiotic prophase I reveals sex-specific features and X chromosome dynamics in human fetal female germline.
title_full_unstemmed	Transcriptional progression during meiotic prophase I reveals sex-specific features and X chromosome dynamics in human fetal female germline.
title_sort	transcriptional progression during meiotic prophase i reveals sex-specific features and x chromosome dynamics in human fetal female germline.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/cc6bb2ecaa424a22922367940bd877c2
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Transcriptional progression during meiotic prophase I reveals sex-specific features and X chromosome dynamics in human fetal female germline.

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