Optimizing tissue-clearing conditions based on analysis of the critical factors affecting tissue-clearing procedures

Abstract Tissue-clearing techniques have received great attention for volume imaging and for the potential to be applied in optical diagnosis. In principle, tissue clearing is achieved by reducing light scattering through a combination of lipid removal, size change, and matching of the refractive in...

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Autores principales:	June Hoan Kim, Min Jee Jang, Jungyoon Choi, Eunsoo Lee, Kyung–Deok Song, Jaeho Cho, Keun-Tae Kim, Hyuk-Jin Cha, Woong Sun
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cc6c35ba239d4dca8387211822122073
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spelling	oai:doaj.org-article:cc6c35ba239d4dca83872118221220732021-12-02T11:40:53ZOptimizing tissue-clearing conditions based on analysis of the critical factors affecting tissue-clearing procedures10.1038/s41598-018-31153-72045-2322https://doaj.org/article/cc6c35ba239d4dca83872118221220732018-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-31153-7https://doaj.org/toc/2045-2322Abstract Tissue-clearing techniques have received great attention for volume imaging and for the potential to be applied in optical diagnosis. In principle, tissue clearing is achieved by reducing light scattering through a combination of lipid removal, size change, and matching of the refractive index (RI) between the imaging solution and the tissue. However, the contributions of these major factors in tissue clearing have not been systematically evaluated yet. In this study, we experimentally measured and mathematically calculated the contribution of these factors to the clearing of four organs (brain, liver, kidney, and lung). We found that these factors differentially influence the maximal clearing efficacy of tissues and the diffusivity of materials inside the tissue. We propose that these physical properties of organs can be utilized for the quality control (Q/C) process during tissue clearing, as well as for the monitoring of the pathological changes of tissues.June Hoan KimMin Jee JangJungyoon ChoiEunsoo LeeKyung–Deok SongJaeho ChoKeun-Tae KimHyuk-Jin ChaWoong SunNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q June Hoan Kim Min Jee Jang Jungyoon Choi Eunsoo Lee Kyung–Deok Song Jaeho Cho Keun-Tae Kim Hyuk-Jin Cha Woong Sun Optimizing tissue-clearing conditions based on analysis of the critical factors affecting tissue-clearing procedures
description	Abstract Tissue-clearing techniques have received great attention for volume imaging and for the potential to be applied in optical diagnosis. In principle, tissue clearing is achieved by reducing light scattering through a combination of lipid removal, size change, and matching of the refractive index (RI) between the imaging solution and the tissue. However, the contributions of these major factors in tissue clearing have not been systematically evaluated yet. In this study, we experimentally measured and mathematically calculated the contribution of these factors to the clearing of four organs (brain, liver, kidney, and lung). We found that these factors differentially influence the maximal clearing efficacy of tissues and the diffusivity of materials inside the tissue. We propose that these physical properties of organs can be utilized for the quality control (Q/C) process during tissue clearing, as well as for the monitoring of the pathological changes of tissues.
format	article
author	June Hoan Kim Min Jee Jang Jungyoon Choi Eunsoo Lee Kyung–Deok Song Jaeho Cho Keun-Tae Kim Hyuk-Jin Cha Woong Sun
author_facet	June Hoan Kim Min Jee Jang Jungyoon Choi Eunsoo Lee Kyung–Deok Song Jaeho Cho Keun-Tae Kim Hyuk-Jin Cha Woong Sun
author_sort	June Hoan Kim
title	Optimizing tissue-clearing conditions based on analysis of the critical factors affecting tissue-clearing procedures
title_short	Optimizing tissue-clearing conditions based on analysis of the critical factors affecting tissue-clearing procedures
title_full	Optimizing tissue-clearing conditions based on analysis of the critical factors affecting tissue-clearing procedures
title_fullStr	Optimizing tissue-clearing conditions based on analysis of the critical factors affecting tissue-clearing procedures
title_full_unstemmed	Optimizing tissue-clearing conditions based on analysis of the critical factors affecting tissue-clearing procedures
title_sort	optimizing tissue-clearing conditions based on analysis of the critical factors affecting tissue-clearing procedures
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/cc6c35ba239d4dca8387211822122073
work_keys_str_mv	AT junehoankim optimizingtissueclearingconditionsbasedonanalysisofthecriticalfactorsaffectingtissueclearingprocedures AT minjeejang optimizingtissueclearingconditionsbasedonanalysisofthecriticalfactorsaffectingtissueclearingprocedures AT jungyoonchoi optimizingtissueclearingconditionsbasedonanalysisofthecriticalfactorsaffectingtissueclearingprocedures AT eunsoolee optimizingtissueclearingconditionsbasedonanalysisofthecriticalfactorsaffectingtissueclearingprocedures AT kyungdeoksong optimizingtissueclearingconditionsbasedonanalysisofthecriticalfactorsaffectingtissueclearingprocedures AT jaehocho optimizingtissueclearingconditionsbasedonanalysisofthecriticalfactorsaffectingtissueclearingprocedures AT keuntaekim optimizingtissueclearingconditionsbasedonanalysisofthecriticalfactorsaffectingtissueclearingprocedures AT hyukjincha optimizingtissueclearingconditionsbasedonanalysisofthecriticalfactorsaffectingtissueclearingprocedures AT woongsun optimizingtissueclearingconditionsbasedonanalysisofthecriticalfactorsaffectingtissueclearingprocedures
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Optimizing tissue-clearing conditions based on analysis of the critical factors affecting tissue-clearing procedures

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