Trials For Preparation And Evaluation Of Inactivated Tissue Culture Newcastle Disease Vaccine From Recent Isolate

The present study was undertaken to development of BHK-21 cell adapted inactivated vaccine of Newcastle disease virus (NDVgenotype VII) from the field isolate from broiler chicken in Egypt during 2015-2016. The isolatesof El-Giza/2015were classified by sequencing as velogenic NDV genotype VII d cont...

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Autores principales:	Amani A. Saleh, Nada A. Fathy, Mohamed A. AbdRabo, Noha A. Helmy
Formato:	article
Lenguaje:	EN
Publicado:	Egyptian Society for Animal Management 2019
Materias:	recent isolate isa ndv pcr Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
Acceso en línea:	https://dx.doi.org/10.21608/javs.2019.62675 https://doaj.org/article/cc7e8672b5a741089b343b88045c499f
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spelling	oai:doaj.org-article:cc7e8672b5a741089b343b88045c499f2021-12-02T16:33:48ZTrials For Preparation And Evaluation Of Inactivated Tissue Culture Newcastle Disease Vaccine From Recent Isolatehttps://dx.doi.org/10.21608/javs.2019.626751687-40722090-3308https://doaj.org/article/cc7e8672b5a741089b343b88045c499f2019-04-01T00:00:00Zhttps://javs.journals.ekb.eg/article_62675.htmlhttps://doaj.org/toc/1687-4072https://doaj.org/toc/2090-3308The present study was undertaken to development of BHK-21 cell adapted inactivated vaccine of Newcastle disease virus (NDVgenotype VII) from the field isolate from broiler chicken in Egypt during 2015-2016. The isolatesof El-Giza/2015were classified by sequencing as velogenic NDV genotype VII d contains F protein cleavage site motifs (112RRQKRF117). Such virus was propagated in the BHK-21 cell line. and cell adapted virus was confirmed as NDV by reverse transcription-polymerase chain reaction (RT-PCR) using fusion gene-specific primers and used to develop inactivated vaccine adjuvanted with Montanide IMS 1313.Potency test revealed that Vaccinated chicks with 0.5ml of prepared NDV vaccine exhibited HI antibody titer of 8.6 log2 three weeks post vaccination with the highest titer (10.6 log2/ml) at the 6th-week post vaccination, and 3rd weeks post challenge test. Protective antibodies values were persisting till 12th weeks post vaccination. All chicken groups vaccinated with both prepared inactivated tissue culture vaccine using ISA 1313 and VSVRI inactivated ISA70 adjuvant vaccines were passed challenge test (97.5%,97%,96% protective efficiency to SPF chickens) against the isolated virulent NDV, while the control group could not provide any protective efficiency. The present study indicated that, BHK-21 cell adapted recent isolated NDV inactivated vaccine produced a satisfactory antibodies titre that efficient in control of the disease in Egypt.Amani A. SalehNada A. FathyMohamed A. AbdRaboNoha A. HelmyEgyptian Society for Animal Managementarticlerecent isolateisandvpcrZoologyQL1-991Veterinary medicineSF600-1100Animal biochemistryQP501-801ENJournal of Applied Veterinary Sciences, Vol 4, Iss 1, Pp 35-42 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	recent isolate isa ndv pcr Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
spellingShingle	recent isolate isa ndv pcr Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801 Amani A. Saleh Nada A. Fathy Mohamed A. AbdRabo Noha A. Helmy Trials For Preparation And Evaluation Of Inactivated Tissue Culture Newcastle Disease Vaccine From Recent Isolate
description	The present study was undertaken to development of BHK-21 cell adapted inactivated vaccine of Newcastle disease virus (NDVgenotype VII) from the field isolate from broiler chicken in Egypt during 2015-2016. The isolatesof El-Giza/2015were classified by sequencing as velogenic NDV genotype VII d contains F protein cleavage site motifs (112RRQKRF117). Such virus was propagated in the BHK-21 cell line. and cell adapted virus was confirmed as NDV by reverse transcription-polymerase chain reaction (RT-PCR) using fusion gene-specific primers and used to develop inactivated vaccine adjuvanted with Montanide IMS 1313.Potency test revealed that Vaccinated chicks with 0.5ml of prepared NDV vaccine exhibited HI antibody titer of 8.6 log2 three weeks post vaccination with the highest titer (10.6 log2/ml) at the 6th-week post vaccination, and 3rd weeks post challenge test. Protective antibodies values were persisting till 12th weeks post vaccination. All chicken groups vaccinated with both prepared inactivated tissue culture vaccine using ISA 1313 and VSVRI inactivated ISA70 adjuvant vaccines were passed challenge test (97.5%,97%,96% protective efficiency to SPF chickens) against the isolated virulent NDV, while the control group could not provide any protective efficiency. The present study indicated that, BHK-21 cell adapted recent isolated NDV inactivated vaccine produced a satisfactory antibodies titre that efficient in control of the disease in Egypt.
format	article
author	Amani A. Saleh Nada A. Fathy Mohamed A. AbdRabo Noha A. Helmy
author_facet	Amani A. Saleh Nada A. Fathy Mohamed A. AbdRabo Noha A. Helmy
author_sort	Amani A. Saleh
title	Trials For Preparation And Evaluation Of Inactivated Tissue Culture Newcastle Disease Vaccine From Recent Isolate
title_short	Trials For Preparation And Evaluation Of Inactivated Tissue Culture Newcastle Disease Vaccine From Recent Isolate
title_full	Trials For Preparation And Evaluation Of Inactivated Tissue Culture Newcastle Disease Vaccine From Recent Isolate
title_fullStr	Trials For Preparation And Evaluation Of Inactivated Tissue Culture Newcastle Disease Vaccine From Recent Isolate
title_full_unstemmed	Trials For Preparation And Evaluation Of Inactivated Tissue Culture Newcastle Disease Vaccine From Recent Isolate
title_sort	trials for preparation and evaluation of inactivated tissue culture newcastle disease vaccine from recent isolate
publisher	Egyptian Society for Animal Management
publishDate	2019
url	https://dx.doi.org/10.21608/javs.2019.62675 https://doaj.org/article/cc7e8672b5a741089b343b88045c499f
work_keys_str_mv	AT amaniasaleh trialsforpreparationandevaluationofinactivatedtissueculturenewcastlediseasevaccinefromrecentisolate AT nadaafathy trialsforpreparationandevaluationofinactivatedtissueculturenewcastlediseasevaccinefromrecentisolate AT mohamedaabdrabo trialsforpreparationandevaluationofinactivatedtissueculturenewcastlediseasevaccinefromrecentisolate AT nohaahelmy trialsforpreparationandevaluationofinactivatedtissueculturenewcastlediseasevaccinefromrecentisolate
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Trials For Preparation And Evaluation Of Inactivated Tissue Culture Newcastle Disease Vaccine From Recent Isolate

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