Effects of Interaction of Protein Components of erythrocyte Membrane in Healthy Individuals and Patients with Ulcerative Colitis

Background. It is known that in ulcerative colitis there is a violation of the barrier function of the colon mucosa, which can lead to pathological changes inside cells, including in the membranes of red blood cells. The stability of the erythrocyte membrane and its ability to deform largely depends...

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Autores principales:	A. S. Sergeeva, Yu. I. Pivovarov, L. A. Dmitrieva, O. V. Sai
Formato:	article
Lenguaje:	RU
Publicado:	Scientific Сentre for Family Health and Human Reproduction Problems 2020
Materias:	erythrocyte membrane proteins ulcerative colitis Science Q
Acceso en línea:	https://doaj.org/article/cc8dec2130734150aa4cb640f0abbb87
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spelling	oai:doaj.org-article:cc8dec2130734150aa4cb640f0abbb872021-11-23T06:14:45ZEffects of Interaction of Protein Components of erythrocyte Membrane in Healthy Individuals and Patients with Ulcerative Colitis2541-94202587-959610.29413/ABS.2020-5.5.5https://doaj.org/article/cc8dec2130734150aa4cb640f0abbb872020-11-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/2438https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-9596Background. It is known that in ulcerative colitis there is a violation of the barrier function of the colon mucosa, which can lead to pathological changes inside cells, including in the membranes of red blood cells. The stability of the erythrocyte membrane and its ability to deform largely depends on the composition and interaction of protein structures inside it. A significant decrease in the content of the main protein components of the erythrocyte membrane in ulcerative colitis was found in comparison with healthy people.Aim of the research. Taking into account the available data, we conducted an additional study to analyze the effects of the relationship between the protein components of the cytoplasmic membrane of erythrocytes in patients with ulcerative colitis and clinically healthy people.Materials and methods. The study involved men and women with ulcerative colitis in the period of exacerbation (n = 51) and clinically healthy individuals matched by sex and age (n = 30). The analysis of the interaction effects of ten main protein components of the erythrocyte membrane was carried out. For each protein under study, its average value was determined, and within the studied groups all proteins were divided into two categories – above and below their average level. Analysis of variance was used to quantify the contribution of each identified factor.Results. It was found that the effect of the relationship is not inherent in all categories of protein components. In ulcerative colitis, the effects of interaction with such important structural proteins as spectrins and ankyrin disappear. In the effect of band 4.1 protein, in contrast to clinically healthy individuals, a new independent factor appears – glyceraldehyde-3-phosphate dehydrogenase, and in the effect on actin – an anion-transport protein.Conclusion. In ulcerative colitis, significant changes occur in the membrane of red blood cells, leading to a violation of the relationships between proteins, a decrease in the total content of the studied proteins and the loss of some of the effects of independent factors on the level of certain protein structures. Based on the findings, adjustments can be made to the treatment of ulcerative colitis in order to strengthen the erythrocyte membrane.A. S. SergeevaYu. I. PivovarovL. A. DmitrievaO. V. SaiScientific Сentre for Family Health and Human Reproduction Problemsarticleerythrocytemembrane proteinsulcerative colitisScienceQRUActa Biomedica Scientifica, Vol 5, Iss 5, Pp 39-46 (2020)
institution	DOAJ
collection	DOAJ
language	RU
topic	erythrocyte membrane proteins ulcerative colitis Science Q
spellingShingle	erythrocyte membrane proteins ulcerative colitis Science Q A. S. Sergeeva Yu. I. Pivovarov L. A. Dmitrieva O. V. Sai Effects of Interaction of Protein Components of erythrocyte Membrane in Healthy Individuals and Patients with Ulcerative Colitis
description	Background. It is known that in ulcerative colitis there is a violation of the barrier function of the colon mucosa, which can lead to pathological changes inside cells, including in the membranes of red blood cells. The stability of the erythrocyte membrane and its ability to deform largely depends on the composition and interaction of protein structures inside it. A significant decrease in the content of the main protein components of the erythrocyte membrane in ulcerative colitis was found in comparison with healthy people.Aim of the research. Taking into account the available data, we conducted an additional study to analyze the effects of the relationship between the protein components of the cytoplasmic membrane of erythrocytes in patients with ulcerative colitis and clinically healthy people.Materials and methods. The study involved men and women with ulcerative colitis in the period of exacerbation (n = 51) and clinically healthy individuals matched by sex and age (n = 30). The analysis of the interaction effects of ten main protein components of the erythrocyte membrane was carried out. For each protein under study, its average value was determined, and within the studied groups all proteins were divided into two categories – above and below their average level. Analysis of variance was used to quantify the contribution of each identified factor.Results. It was found that the effect of the relationship is not inherent in all categories of protein components. In ulcerative colitis, the effects of interaction with such important structural proteins as spectrins and ankyrin disappear. In the effect of band 4.1 protein, in contrast to clinically healthy individuals, a new independent factor appears – glyceraldehyde-3-phosphate dehydrogenase, and in the effect on actin – an anion-transport protein.Conclusion. In ulcerative colitis, significant changes occur in the membrane of red blood cells, leading to a violation of the relationships between proteins, a decrease in the total content of the studied proteins and the loss of some of the effects of independent factors on the level of certain protein structures. Based on the findings, adjustments can be made to the treatment of ulcerative colitis in order to strengthen the erythrocyte membrane.
format	article
author	A. S. Sergeeva Yu. I. Pivovarov L. A. Dmitrieva O. V. Sai
author_facet	A. S. Sergeeva Yu. I. Pivovarov L. A. Dmitrieva O. V. Sai
author_sort	A. S. Sergeeva
title	Effects of Interaction of Protein Components of erythrocyte Membrane in Healthy Individuals and Patients with Ulcerative Colitis
title_short	Effects of Interaction of Protein Components of erythrocyte Membrane in Healthy Individuals and Patients with Ulcerative Colitis
title_full	Effects of Interaction of Protein Components of erythrocyte Membrane in Healthy Individuals and Patients with Ulcerative Colitis
title_fullStr	Effects of Interaction of Protein Components of erythrocyte Membrane in Healthy Individuals and Patients with Ulcerative Colitis
title_full_unstemmed	Effects of Interaction of Protein Components of erythrocyte Membrane in Healthy Individuals and Patients with Ulcerative Colitis
title_sort	effects of interaction of protein components of erythrocyte membrane in healthy individuals and patients with ulcerative colitis
publisher	Scientific Сentre for Family Health and Human Reproduction Problems
publishDate	2020
url	https://doaj.org/article/cc8dec2130734150aa4cb640f0abbb87
work_keys_str_mv	AT assergeeva effectsofinteractionofproteincomponentsoferythrocytemembraneinhealthyindividualsandpatientswithulcerativecolitis AT yuipivovarov effectsofinteractionofproteincomponentsoferythrocytemembraneinhealthyindividualsandpatientswithulcerativecolitis AT ladmitrieva effectsofinteractionofproteincomponentsoferythrocytemembraneinhealthyindividualsandpatientswithulcerativecolitis AT ovsai effectsofinteractionofproteincomponentsoferythrocytemembraneinhealthyindividualsandpatientswithulcerativecolitis
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Effects of Interaction of Protein Components of erythrocyte Membrane in Healthy Individuals and Patients with Ulcerative Colitis

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