Different pathways involved in the stimulatory effects of homocysteine on rat duodenal smooth muscle

Recent studies have confirmed that hyperhomocysteinemia is associated with gastrointestinal diseases; however, the direct effect of homocysteine on gastrointestinal reactivity still remains unknown. The aim of this study was to demonstrate how homocysteine may affect nitric oxide mediated duodenal r...

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Autores principales:	Stojanović Marija, Šćepanović Ljiljana, Mitrović Dušan, Šćepanović Vuk, Šćepanović Radomir, Djuric Marko, Ilić Slobodan, Šćepanović Teja, Djuric Dragan
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2017
Materias:	contractility duodenum homocysteine oxidative stress rat Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/cc8e605a22df4af0a9425d3da490be49
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spelling	oai:doaj.org-article:cc8e605a22df4af0a9425d3da490be492021-11-17T21:27:51ZDifferent pathways involved in the stimulatory effects of homocysteine on rat duodenal smooth muscle1820-744810.1515/acve-2017-0021https://doaj.org/article/cc8e605a22df4af0a9425d3da490be492017-06-01T00:00:00Zhttps://doi.org/10.1515/acve-2017-0021https://doaj.org/toc/1820-7448Recent studies have confirmed that hyperhomocysteinemia is associated with gastrointestinal diseases; however, the direct effect of homocysteine on gastrointestinal reactivity still remains unknown. The aim of this study was to demonstrate how homocysteine may affect nitric oxide mediated duodenal relaxation and whether cholinergic receptors and K+ channels take part in stimulating motility, as well as to explore whether oxidative stress is associated with homocysteine-mediated effects. Experiments were carried out on male rats, body mass 250-300 g. Two groups of animals were treated by i.p. application of saline and D,L-Hcy (0.6 μmol/g bm). After 2h of incubation, the duodenal segments were prepared for biochemical analysis and contractile response measurements in an organ bath with Tyrode’s solution. Effects of TEA (10 mmol/L) and L-NAME (30 μmol/L) on duodenal contractility in the presence of D,L-Hcy (0.6 μmol/g bm) were investigated. Elevated homocysteine levels seem to be of crucial importance for the deterioration of contractility through nitric oxide mediated relaxation, and, in part, by activation of K+ channels. Hcy showed direct promuscarinic effects, since 30 min pretreatment of rat duodenum significantly enhanced the contractile effect of increasing concentrations of ACh (10−9-10−2 mol/L). Catalase activity, superoxide dismutase, glutathione peroxidase and the total antioxidant system were reduced while the thiobarbituric acid-reactive substances level was elevated. Our data showed a consistent profile of gastrointestinal injury elicited by sulfur-containing amino acid-homocysteine. This could contribute to explain, at least in part, the mechanisms involved in human gastrointestinal diseases associated to hyperhomocysteinemia.Stojanović MarijaŠćepanović LjiljanaMitrović DušanŠćepanović VukŠćepanović RadomirDjuric MarkoIlić SlobodanŠćepanović TejaDjuric DraganSciendoarticlecontractilityduodenumhomocysteineoxidative stressratVeterinary medicineSF600-1100ENActa Veterinaria, Vol 67, Iss 2, Pp 254-270 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	contractility duodenum homocysteine oxidative stress rat Veterinary medicine SF600-1100
spellingShingle	contractility duodenum homocysteine oxidative stress rat Veterinary medicine SF600-1100 Stojanović Marija Šćepanović Ljiljana Mitrović Dušan Šćepanović Vuk Šćepanović Radomir Djuric Marko Ilić Slobodan Šćepanović Teja Djuric Dragan Different pathways involved in the stimulatory effects of homocysteine on rat duodenal smooth muscle
description	Recent studies have confirmed that hyperhomocysteinemia is associated with gastrointestinal diseases; however, the direct effect of homocysteine on gastrointestinal reactivity still remains unknown. The aim of this study was to demonstrate how homocysteine may affect nitric oxide mediated duodenal relaxation and whether cholinergic receptors and K+ channels take part in stimulating motility, as well as to explore whether oxidative stress is associated with homocysteine-mediated effects. Experiments were carried out on male rats, body mass 250-300 g. Two groups of animals were treated by i.p. application of saline and D,L-Hcy (0.6 μmol/g bm). After 2h of incubation, the duodenal segments were prepared for biochemical analysis and contractile response measurements in an organ bath with Tyrode’s solution. Effects of TEA (10 mmol/L) and L-NAME (30 μmol/L) on duodenal contractility in the presence of D,L-Hcy (0.6 μmol/g bm) were investigated. Elevated homocysteine levels seem to be of crucial importance for the deterioration of contractility through nitric oxide mediated relaxation, and, in part, by activation of K+ channels. Hcy showed direct promuscarinic effects, since 30 min pretreatment of rat duodenum significantly enhanced the contractile effect of increasing concentrations of ACh (10−9-10−2 mol/L). Catalase activity, superoxide dismutase, glutathione peroxidase and the total antioxidant system were reduced while the thiobarbituric acid-reactive substances level was elevated. Our data showed a consistent profile of gastrointestinal injury elicited by sulfur-containing amino acid-homocysteine. This could contribute to explain, at least in part, the mechanisms involved in human gastrointestinal diseases associated to hyperhomocysteinemia.
format	article
author	Stojanović Marija Šćepanović Ljiljana Mitrović Dušan Šćepanović Vuk Šćepanović Radomir Djuric Marko Ilić Slobodan Šćepanović Teja Djuric Dragan
author_facet	Stojanović Marija Šćepanović Ljiljana Mitrović Dušan Šćepanović Vuk Šćepanović Radomir Djuric Marko Ilić Slobodan Šćepanović Teja Djuric Dragan
author_sort	Stojanović Marija
title	Different pathways involved in the stimulatory effects of homocysteine on rat duodenal smooth muscle
title_short	Different pathways involved in the stimulatory effects of homocysteine on rat duodenal smooth muscle
title_full	Different pathways involved in the stimulatory effects of homocysteine on rat duodenal smooth muscle
title_fullStr	Different pathways involved in the stimulatory effects of homocysteine on rat duodenal smooth muscle
title_full_unstemmed	Different pathways involved in the stimulatory effects of homocysteine on rat duodenal smooth muscle
title_sort	different pathways involved in the stimulatory effects of homocysteine on rat duodenal smooth muscle
publisher	Sciendo
publishDate	2017
url	https://doaj.org/article/cc8e605a22df4af0a9425d3da490be49
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Different pathways involved in the stimulatory effects of homocysteine on rat duodenal smooth muscle

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