Cordycepin Nanoencapsulated in Poly(Lactic-Co-Glycolic Acid) Exhibits Better Cytotoxicity and Lower Hemotoxicity Than Free Drug

Gregory Marslin,1– 3 Vinoth Khandelwal,4 Gregory Franklin5 1School of Pharmacy, Sathyabama Institute of Science and Technology, Jeppiaar Nagar, Rajiv Gandhi Salai, Chennai 600119, India; 2Ratnam Institute of Pharmacy and Research, Nellore, 524346, India; 3College of Biological Science and...

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Autores principales:	Marslin G, Khandelwal V, Franklin G
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	cordycepin nanoparticles poly lactic-co-glycolic acid cellular uptake cytotoxicity breast cancer cells hemolysis Medical technology R855-855.5 Chemical technology TP1-1185
Acceso en línea:	https://doaj.org/article/cc93d4a18e9a43dea994493ec32cad59
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spelling	oai:doaj.org-article:cc93d4a18e9a43dea994493ec32cad592021-12-02T10:23:05ZCordycepin Nanoencapsulated in Poly(Lactic-Co-Glycolic Acid) Exhibits Better Cytotoxicity and Lower Hemotoxicity Than Free Drug1177-8903https://doaj.org/article/cc93d4a18e9a43dea994493ec32cad592020-06-01T00:00:00Zhttps://www.dovepress.com/cordycepin-nanoencapsulated-in-polylactic-co-glycolic-acid-exhibits-be-peer-reviewed-article-NSAhttps://doaj.org/toc/1177-8903Gregory Marslin,1– 3 Vinoth Khandelwal,4 Gregory Franklin5 1School of Pharmacy, Sathyabama Institute of Science and Technology, Jeppiaar Nagar, Rajiv Gandhi Salai, Chennai 600119, India; 2Ratnam Institute of Pharmacy and Research, Nellore, 524346, India; 3College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong, Shaanxi, People’s Republic of China; 4Department of Translational Pharmacology, Santa Maria Imbaro, Italy; 5Institute of Plant Genetics of the Polish Academy of Sciencs, Strzeszyńska 34, Poznań 60-479, Wielkopolska, PolandCorrespondence: Gregory Marslin; Gregory Franklin Tel +91 4424502344Email marslingregory@gmail.com; fgre@igr.poznan.plPurpose: Cordycepin, a natural product isolated from the fungus Cordyceps militaris, is a potential candidate for breast cancer therapy. However, due to its structural similarity with adenosine, cordycepin is rapidly metabolized into an inactive form in the body, hindering its development as a therapeutic agent. In the present study, we have prepared cordycepin as nanoparticles in poly(lactic-co-glycolic acid) (PLGA) and compared their cellular uptake, cytotoxicity and hemolytic potential with free cordycepin.Materials and Methods: Cordycepin-loaded PLGA nanoparticles (CPNPs) were prepared by the double-emulsion solvent evaporation method. Physico-chemical characterization of the nanoparticles was done by zetasizer, transmission electron microscopy (TEM) and reverse-phase high-pressure liquid chromatography (RP-HPLC) analyses. Cellular uptake and cytotoxicity of CPNPs and free drug were tested in human breast cancer cells (MCF7). Hemolytic potential of both of these forms was evaluated in rat red blood cells (RBCs).Results: Physico-chemical characterization revealed that CPNPs were spherical in shape, possessed a size range of 179– 246 nm, and released the encapsulated drug sustainably over a period of 10 days. CPNPs exhibited a high level of cellular uptake and cytotoxicity than the free drug in MCF-7 cells. While CPNPs were not toxic to rat RBCs even at high concentrations, free cordycepin induced hemolysis of these cells at relatively low concentration.Conclusion: Our results reveal that delivery as CPNPs could enhance the clinical efficacy of cordycepin substantially.Keywords: cordycepin, nanoparticles, PLGA, cellular uptake, cytotoxicity, breast cancer cells, hemolysisMarslin GKhandelwal VFranklin GDove Medical Pressarticlecordycepinnanoparticlespoly lactic-co-glycolic acidcellular uptakecytotoxicitybreast cancer cellshemolysisMedical technologyR855-855.5Chemical technologyTP1-1185ENNanotechnology, Science and Applications, Vol Volume 13, Pp 37-45 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	cordycepin nanoparticles poly lactic-co-glycolic acid cellular uptake cytotoxicity breast cancer cells hemolysis Medical technology R855-855.5 Chemical technology TP1-1185
spellingShingle	cordycepin nanoparticles poly lactic-co-glycolic acid cellular uptake cytotoxicity breast cancer cells hemolysis Medical technology R855-855.5 Chemical technology TP1-1185 Marslin G Khandelwal V Franklin G Cordycepin Nanoencapsulated in Poly(Lactic-Co-Glycolic Acid) Exhibits Better Cytotoxicity and Lower Hemotoxicity Than Free Drug
description	Gregory Marslin,1– 3 Vinoth Khandelwal,4 Gregory Franklin5 1School of Pharmacy, Sathyabama Institute of Science and Technology, Jeppiaar Nagar, Rajiv Gandhi Salai, Chennai 600119, India; 2Ratnam Institute of Pharmacy and Research, Nellore, 524346, India; 3College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong, Shaanxi, People’s Republic of China; 4Department of Translational Pharmacology, Santa Maria Imbaro, Italy; 5Institute of Plant Genetics of the Polish Academy of Sciencs, Strzeszyńska 34, Poznań 60-479, Wielkopolska, PolandCorrespondence: Gregory Marslin; Gregory Franklin Tel +91 4424502344Email marslingregory@gmail.com; fgre@igr.poznan.plPurpose: Cordycepin, a natural product isolated from the fungus Cordyceps militaris, is a potential candidate for breast cancer therapy. However, due to its structural similarity with adenosine, cordycepin is rapidly metabolized into an inactive form in the body, hindering its development as a therapeutic agent. In the present study, we have prepared cordycepin as nanoparticles in poly(lactic-co-glycolic acid) (PLGA) and compared their cellular uptake, cytotoxicity and hemolytic potential with free cordycepin.Materials and Methods: Cordycepin-loaded PLGA nanoparticles (CPNPs) were prepared by the double-emulsion solvent evaporation method. Physico-chemical characterization of the nanoparticles was done by zetasizer, transmission electron microscopy (TEM) and reverse-phase high-pressure liquid chromatography (RP-HPLC) analyses. Cellular uptake and cytotoxicity of CPNPs and free drug were tested in human breast cancer cells (MCF7). Hemolytic potential of both of these forms was evaluated in rat red blood cells (RBCs).Results: Physico-chemical characterization revealed that CPNPs were spherical in shape, possessed a size range of 179– 246 nm, and released the encapsulated drug sustainably over a period of 10 days. CPNPs exhibited a high level of cellular uptake and cytotoxicity than the free drug in MCF-7 cells. While CPNPs were not toxic to rat RBCs even at high concentrations, free cordycepin induced hemolysis of these cells at relatively low concentration.Conclusion: Our results reveal that delivery as CPNPs could enhance the clinical efficacy of cordycepin substantially.Keywords: cordycepin, nanoparticles, PLGA, cellular uptake, cytotoxicity, breast cancer cells, hemolysis
format	article
author	Marslin G Khandelwal V Franklin G
author_facet	Marslin G Khandelwal V Franklin G
author_sort	Marslin G
title	Cordycepin Nanoencapsulated in Poly(Lactic-Co-Glycolic Acid) Exhibits Better Cytotoxicity and Lower Hemotoxicity Than Free Drug
title_short	Cordycepin Nanoencapsulated in Poly(Lactic-Co-Glycolic Acid) Exhibits Better Cytotoxicity and Lower Hemotoxicity Than Free Drug
title_full	Cordycepin Nanoencapsulated in Poly(Lactic-Co-Glycolic Acid) Exhibits Better Cytotoxicity and Lower Hemotoxicity Than Free Drug
title_fullStr	Cordycepin Nanoencapsulated in Poly(Lactic-Co-Glycolic Acid) Exhibits Better Cytotoxicity and Lower Hemotoxicity Than Free Drug
title_full_unstemmed	Cordycepin Nanoencapsulated in Poly(Lactic-Co-Glycolic Acid) Exhibits Better Cytotoxicity and Lower Hemotoxicity Than Free Drug
title_sort	cordycepin nanoencapsulated in poly(lactic-co-glycolic acid) exhibits better cytotoxicity and lower hemotoxicity than free drug
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/cc93d4a18e9a43dea994493ec32cad59
work_keys_str_mv	AT marsling cordycepinnanoencapsulatedinpolylacticcoglycolicacidexhibitsbettercytotoxicityandlowerhemotoxicitythanfreedrug AT khandelwalv cordycepinnanoencapsulatedinpolylacticcoglycolicacidexhibitsbettercytotoxicityandlowerhemotoxicitythanfreedrug AT frankling cordycepinnanoencapsulatedinpolylacticcoglycolicacidexhibitsbettercytotoxicityandlowerhemotoxicitythanfreedrug
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Cordycepin Nanoencapsulated in Poly(Lactic-Co-Glycolic Acid) Exhibits Better Cytotoxicity and Lower Hemotoxicity Than Free Drug

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