Novel bioimaging techniques of metals by laser ablation inductively coupled plasma mass spectrometry for diagnosis of fibrotic and cirrhotic liver disorders.

<h4>Background and aims</h4>Hereditary disorders associated with metal overload or unwanted toxic accumulation of heavy metals can lead to morbidity and mortality. Patients with hereditary hemochromatosis or Wilson disease for example may develop severe hepatic pathology including fibros...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Pornwilard M-M, Ralf Weiskirchen, Nikolaus Gassler, Anja K Bosserhoff, J Sabine Becker
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cc96ffea0cc440bdbed3447dd5f9a95f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:cc96ffea0cc440bdbed3447dd5f9a95f
record_format	dspace
spelling	oai:doaj.org-article:cc96ffea0cc440bdbed3447dd5f9a95f2021-11-18T07:54:18ZNovel bioimaging techniques of metals by laser ablation inductively coupled plasma mass spectrometry for diagnosis of fibrotic and cirrhotic liver disorders.1932-620310.1371/journal.pone.0058702https://doaj.org/article/cc96ffea0cc440bdbed3447dd5f9a95f2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23505552/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background and aims</h4>Hereditary disorders associated with metal overload or unwanted toxic accumulation of heavy metals can lead to morbidity and mortality. Patients with hereditary hemochromatosis or Wilson disease for example may develop severe hepatic pathology including fibrosis, cirrhosis or hepatocellular carcinoma. While relevant disease genes are identified and genetic testing is applicable, liver biopsy in combination with metal detecting techniques such as energy-dispersive X-ray spectroscopy (EDX) is still applied for accurate diagnosis of metals. Vice versa, several metals are needed in trace amounts for carrying out vital functions and their deficiency due to rapid growth, pregnancy, excessive blood loss, and insufficient nutritional or digestive uptake results in organic and systemic shortcomings. Established in situ techniques, such as EDX-ray spectroscopy, are not sensitive enough to analyze trace metal distribution and the quantification of metal images is difficult.<h4>Methods</h4>In this study, we developed a quantitative biometal imaging technique of human liver tissue by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) in order to compare the distribution of selected metals in cryo-sections of healthy and fibrotic/cirrhotic livers.<h4>Results</h4>Most of the metals are homogeneous distributed within the normal tissue, while they are redirected within fibrotic livers resulting in significant metal deposits. Moreover, total iron and copper concentrations in diseased liver were found about 3-5 times higher than in normal liver samples.<h4>Conclusions</h4>Biometal imaging via LA-ICP-MS is a sensitive innovative diagnostic tool that will impact clinical practice in identification and evaluation of hepatic metal disorders and to detect subtle metal variations during ongoing hepatic fibrogenesis.Pornwilard M-MRalf WeiskirchenNikolaus GasslerAnja K BosserhoffJ Sabine BeckerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 3, p e58702 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Pornwilard M-M Ralf Weiskirchen Nikolaus Gassler Anja K Bosserhoff J Sabine Becker Novel bioimaging techniques of metals by laser ablation inductively coupled plasma mass spectrometry for diagnosis of fibrotic and cirrhotic liver disorders.
description	<h4>Background and aims</h4>Hereditary disorders associated with metal overload or unwanted toxic accumulation of heavy metals can lead to morbidity and mortality. Patients with hereditary hemochromatosis or Wilson disease for example may develop severe hepatic pathology including fibrosis, cirrhosis or hepatocellular carcinoma. While relevant disease genes are identified and genetic testing is applicable, liver biopsy in combination with metal detecting techniques such as energy-dispersive X-ray spectroscopy (EDX) is still applied for accurate diagnosis of metals. Vice versa, several metals are needed in trace amounts for carrying out vital functions and their deficiency due to rapid growth, pregnancy, excessive blood loss, and insufficient nutritional or digestive uptake results in organic and systemic shortcomings. Established in situ techniques, such as EDX-ray spectroscopy, are not sensitive enough to analyze trace metal distribution and the quantification of metal images is difficult.<h4>Methods</h4>In this study, we developed a quantitative biometal imaging technique of human liver tissue by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) in order to compare the distribution of selected metals in cryo-sections of healthy and fibrotic/cirrhotic livers.<h4>Results</h4>Most of the metals are homogeneous distributed within the normal tissue, while they are redirected within fibrotic livers resulting in significant metal deposits. Moreover, total iron and copper concentrations in diseased liver were found about 3-5 times higher than in normal liver samples.<h4>Conclusions</h4>Biometal imaging via LA-ICP-MS is a sensitive innovative diagnostic tool that will impact clinical practice in identification and evaluation of hepatic metal disorders and to detect subtle metal variations during ongoing hepatic fibrogenesis.
format	article
author	Pornwilard M-M Ralf Weiskirchen Nikolaus Gassler Anja K Bosserhoff J Sabine Becker
author_facet	Pornwilard M-M Ralf Weiskirchen Nikolaus Gassler Anja K Bosserhoff J Sabine Becker
author_sort	Pornwilard M-M
title	Novel bioimaging techniques of metals by laser ablation inductively coupled plasma mass spectrometry for diagnosis of fibrotic and cirrhotic liver disorders.
title_short	Novel bioimaging techniques of metals by laser ablation inductively coupled plasma mass spectrometry for diagnosis of fibrotic and cirrhotic liver disorders.
title_full	Novel bioimaging techniques of metals by laser ablation inductively coupled plasma mass spectrometry for diagnosis of fibrotic and cirrhotic liver disorders.
title_fullStr	Novel bioimaging techniques of metals by laser ablation inductively coupled plasma mass spectrometry for diagnosis of fibrotic and cirrhotic liver disorders.
title_full_unstemmed	Novel bioimaging techniques of metals by laser ablation inductively coupled plasma mass spectrometry for diagnosis of fibrotic and cirrhotic liver disorders.
title_sort	novel bioimaging techniques of metals by laser ablation inductively coupled plasma mass spectrometry for diagnosis of fibrotic and cirrhotic liver disorders.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/cc96ffea0cc440bdbed3447dd5f9a95f
work_keys_str_mv	AT pornwilardmm novelbioimagingtechniquesofmetalsbylaserablationinductivelycoupledplasmamassspectrometryfordiagnosisoffibroticandcirrhoticliverdisorders AT ralfweiskirchen novelbioimagingtechniquesofmetalsbylaserablationinductivelycoupledplasmamassspectrometryfordiagnosisoffibroticandcirrhoticliverdisorders AT nikolausgassler novelbioimagingtechniquesofmetalsbylaserablationinductivelycoupledplasmamassspectrometryfordiagnosisoffibroticandcirrhoticliverdisorders AT anjakbosserhoff novelbioimagingtechniquesofmetalsbylaserablationinductivelycoupledplasmamassspectrometryfordiagnosisoffibroticandcirrhoticliverdisorders AT jsabinebecker novelbioimagingtechniquesofmetalsbylaserablationinductivelycoupledplasmamassspectrometryfordiagnosisoffibroticandcirrhoticliverdisorders
_version_	1718422796190613504

Novel bioimaging techniques of metals by laser ablation inductively coupled plasma mass spectrometry for diagnosis of fibrotic and cirrhotic liver disorders.

Ejemplares similares