Resonant soft X-ray scattering reveals cellulose microfibril spacing in plant primary cell walls

Abstract Cellulose microfibrils are crucial for many of the remarkable mechanical properties of primary cell walls. Nevertheless, many structural features of cellulose microfibril organization in cell walls are not yet fully described. Microscopy techniques provide direct visualization of cell wall...

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Autores principales:	Dan Ye, Sarah N. Kiemle, Sintu Rongpipi, Xuan Wang, Cheng Wang, Daniel J. Cosgrove, Esther W. Gomez, Enrique D. Gomez
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cc9931e6ba984b87addf70d657b4fbcb
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spelling	oai:doaj.org-article:cc9931e6ba984b87addf70d657b4fbcb2021-12-02T15:08:27ZResonant soft X-ray scattering reveals cellulose microfibril spacing in plant primary cell walls10.1038/s41598-018-31024-12045-2322https://doaj.org/article/cc9931e6ba984b87addf70d657b4fbcb2018-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-31024-1https://doaj.org/toc/2045-2322Abstract Cellulose microfibrils are crucial for many of the remarkable mechanical properties of primary cell walls. Nevertheless, many structural features of cellulose microfibril organization in cell walls are not yet fully described. Microscopy techniques provide direct visualization of cell wall organization, and quantification of some aspects of wall microstructure is possible through image processing. Complementary to microscopy techniques, scattering yields structural information in reciprocal space over large sample areas. Using the onion epidermal wall as a model system, we introduce resonant soft X-ray scattering (RSoXS) to directly quantify the average interfibril spacing. Tuning the X-ray energy to the calcium L-edge enhances the contrast between cellulose and pectin due to the localization of calcium ions to homogalacturonan in the pectin matrix. As a consequence, RSoXS profiles reveal an average center-to-center distance between cellulose microfibrils or microfibril bundles of about 20 nm.Dan YeSarah N. KiemleSintu RongpipiXuan WangCheng WangDaniel J. CosgroveEsther W. GomezEnrique D. GomezNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-13 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dan Ye Sarah N. Kiemle Sintu Rongpipi Xuan Wang Cheng Wang Daniel J. Cosgrove Esther W. Gomez Enrique D. Gomez Resonant soft X-ray scattering reveals cellulose microfibril spacing in plant primary cell walls
description	Abstract Cellulose microfibrils are crucial for many of the remarkable mechanical properties of primary cell walls. Nevertheless, many structural features of cellulose microfibril organization in cell walls are not yet fully described. Microscopy techniques provide direct visualization of cell wall organization, and quantification of some aspects of wall microstructure is possible through image processing. Complementary to microscopy techniques, scattering yields structural information in reciprocal space over large sample areas. Using the onion epidermal wall as a model system, we introduce resonant soft X-ray scattering (RSoXS) to directly quantify the average interfibril spacing. Tuning the X-ray energy to the calcium L-edge enhances the contrast between cellulose and pectin due to the localization of calcium ions to homogalacturonan in the pectin matrix. As a consequence, RSoXS profiles reveal an average center-to-center distance between cellulose microfibrils or microfibril bundles of about 20 nm.
format	article
author	Dan Ye Sarah N. Kiemle Sintu Rongpipi Xuan Wang Cheng Wang Daniel J. Cosgrove Esther W. Gomez Enrique D. Gomez
author_facet	Dan Ye Sarah N. Kiemle Sintu Rongpipi Xuan Wang Cheng Wang Daniel J. Cosgrove Esther W. Gomez Enrique D. Gomez
author_sort	Dan Ye
title	Resonant soft X-ray scattering reveals cellulose microfibril spacing in plant primary cell walls
title_short	Resonant soft X-ray scattering reveals cellulose microfibril spacing in plant primary cell walls
title_full	Resonant soft X-ray scattering reveals cellulose microfibril spacing in plant primary cell walls
title_fullStr	Resonant soft X-ray scattering reveals cellulose microfibril spacing in plant primary cell walls
title_full_unstemmed	Resonant soft X-ray scattering reveals cellulose microfibril spacing in plant primary cell walls
title_sort	resonant soft x-ray scattering reveals cellulose microfibril spacing in plant primary cell walls
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/cc9931e6ba984b87addf70d657b4fbcb
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_version_	1718388149714944000

Resonant soft X-ray scattering reveals cellulose microfibril spacing in plant primary cell walls

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