Morphoregulatory functions of the RNA-binding motif protein 3 in cell spreading, polarity and migration

Abstract RNA-binding proteins are emerging as key regulators of transitions in cell morphology. The RNA-binding motif protein 3 (RBM3) is a cold-inducible RNA-binding protein with broadly relevant roles in cellular protection, and putative functions in cancer and development. Several findings sugges...

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Autores principales:	J. Pilotte, W. Kiosses, S. W. Chan, H. P. Makarenkova, E. Dupont-Versteegden, P. W. Vanderklish
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cca1981e1fb841ea873309b438f1c2b0
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spelling	oai:doaj.org-article:cca1981e1fb841ea873309b438f1c2b02021-12-02T16:08:03ZMorphoregulatory functions of the RNA-binding motif protein 3 in cell spreading, polarity and migration10.1038/s41598-018-25668-22045-2322https://doaj.org/article/cca1981e1fb841ea873309b438f1c2b02018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25668-2https://doaj.org/toc/2045-2322Abstract RNA-binding proteins are emerging as key regulators of transitions in cell morphology. The RNA-binding motif protein 3 (RBM3) is a cold-inducible RNA-binding protein with broadly relevant roles in cellular protection, and putative functions in cancer and development. Several findings suggest that RBM3 has morphoregulatory functions germane to its roles in these contexts. For example, RBM3 helps maintain the morphological integrity of cell protrusions during cell stress and disease. Moreover, it is highly expressed in migrating neurons of the developing brain and in cancer invadopodia, suggesting roles in migration. We here show that RBM3 regulates cell polarity, spreading and migration. RBM3 was present in spreading initiation centers, filopodia and blebs that formed during cell spreading in cell lines and primary myoblasts. Reducing RBM3 triggered exaggerated spreading, increased RhoA expression, and a loss of polarity that was rescued by Rho kinase inhibition and overexpression of CRMP2. High RBM3 expression enhanced the motility of cells migrating by a mesenchymal mode involving extension of long protrusions, whereas RBM3 knockdown slowed migration, greatly reducing the ability of cells to extend protrusions and impairing multiple processes that require directional migration. These data establish novel functions of RBM3 of potential significance to tissue repair, metastasis and development.J. PilotteW. KiossesS. W. ChanH. P. MakarenkovaE. Dupont-VersteegdenP. W. VanderklishNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-19 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q J. Pilotte W. Kiosses S. W. Chan H. P. Makarenkova E. Dupont-Versteegden P. W. Vanderklish Morphoregulatory functions of the RNA-binding motif protein 3 in cell spreading, polarity and migration
description	Abstract RNA-binding proteins are emerging as key regulators of transitions in cell morphology. The RNA-binding motif protein 3 (RBM3) is a cold-inducible RNA-binding protein with broadly relevant roles in cellular protection, and putative functions in cancer and development. Several findings suggest that RBM3 has morphoregulatory functions germane to its roles in these contexts. For example, RBM3 helps maintain the morphological integrity of cell protrusions during cell stress and disease. Moreover, it is highly expressed in migrating neurons of the developing brain and in cancer invadopodia, suggesting roles in migration. We here show that RBM3 regulates cell polarity, spreading and migration. RBM3 was present in spreading initiation centers, filopodia and blebs that formed during cell spreading in cell lines and primary myoblasts. Reducing RBM3 triggered exaggerated spreading, increased RhoA expression, and a loss of polarity that was rescued by Rho kinase inhibition and overexpression of CRMP2. High RBM3 expression enhanced the motility of cells migrating by a mesenchymal mode involving extension of long protrusions, whereas RBM3 knockdown slowed migration, greatly reducing the ability of cells to extend protrusions and impairing multiple processes that require directional migration. These data establish novel functions of RBM3 of potential significance to tissue repair, metastasis and development.
format	article
author	J. Pilotte W. Kiosses S. W. Chan H. P. Makarenkova E. Dupont-Versteegden P. W. Vanderklish
author_facet	J. Pilotte W. Kiosses S. W. Chan H. P. Makarenkova E. Dupont-Versteegden P. W. Vanderklish
author_sort	J. Pilotte
title	Morphoregulatory functions of the RNA-binding motif protein 3 in cell spreading, polarity and migration
title_short	Morphoregulatory functions of the RNA-binding motif protein 3 in cell spreading, polarity and migration
title_full	Morphoregulatory functions of the RNA-binding motif protein 3 in cell spreading, polarity and migration
title_fullStr	Morphoregulatory functions of the RNA-binding motif protein 3 in cell spreading, polarity and migration
title_full_unstemmed	Morphoregulatory functions of the RNA-binding motif protein 3 in cell spreading, polarity and migration
title_sort	morphoregulatory functions of the rna-binding motif protein 3 in cell spreading, polarity and migration
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/cca1981e1fb841ea873309b438f1c2b0
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Morphoregulatory functions of the RNA-binding motif protein 3 in cell spreading, polarity and migration

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