A cell line resource derived from honey bee (Apis mellifera) embryonic tissues.

A major hindrance to the study of honey bee pathogens or the effects of pesticides and nutritional deficiencies is the lack of controlled in vitro culture systems comprised of honey bee cells. Such systems are important to determine the impact of these stress factors on the developmental and cell bi...

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Autores principales:	Michael J Goblirsch, Marla S Spivak, Timothy J Kurtti
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cca8903f8d3d4e6989d72c9f22467b5d
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spelling	oai:doaj.org-article:cca8903f8d3d4e6989d72c9f22467b5d2021-11-18T09:03:32ZA cell line resource derived from honey bee (Apis mellifera) embryonic tissues.1932-620310.1371/journal.pone.0069831https://doaj.org/article/cca8903f8d3d4e6989d72c9f22467b5d2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23894551/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203A major hindrance to the study of honey bee pathogens or the effects of pesticides and nutritional deficiencies is the lack of controlled in vitro culture systems comprised of honey bee cells. Such systems are important to determine the impact of these stress factors on the developmental and cell biology of honey bees. We have developed a method incorporating established insect cell culture techniques that supports sustained growth of honey bee cells in vitro. We used honey bee eggs mid to late in their embryogenesis to establish primary cultures, as these eggs contain cells that are progressively dividing. Primary cultures were initiated in modified Leibovitz's L15 medium and incubated at 32(°)C. Serial transfer of material from several primary cultures was maintained and has led to the isolation of young cell lines. A cell line (AmE-711) has been established that is composed mainly of fibroblast-type cells that form an adherent monolayer. Most cells in the line are diploid (2n = 32) and have the Apis mellifera karyotype as revealed by Giemsa stain. The partial sequence for the mitochondrial-encoded cytochrome c oxidase subunit I (Cox 1) gene in the cell line is identical to those from honey bee tissues and a consensus sequence for A. mellifera. The population doubling time is approximately 4 days. Importantly, the cell line is continuously subcultured every 10-14 days when split at a 1:3 ratio and is cryopreserved in liquid nitrogen. The cell culture system we have developed has potential application for studies aimed at honey bee development, genetics, pathogenesis, transgenesis, and toxicology.Michael J GoblirschMarla S SpivakTimothy J KurttiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 7, p e69831 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Michael J Goblirsch Marla S Spivak Timothy J Kurtti A cell line resource derived from honey bee (Apis mellifera) embryonic tissues.
description	A major hindrance to the study of honey bee pathogens or the effects of pesticides and nutritional deficiencies is the lack of controlled in vitro culture systems comprised of honey bee cells. Such systems are important to determine the impact of these stress factors on the developmental and cell biology of honey bees. We have developed a method incorporating established insect cell culture techniques that supports sustained growth of honey bee cells in vitro. We used honey bee eggs mid to late in their embryogenesis to establish primary cultures, as these eggs contain cells that are progressively dividing. Primary cultures were initiated in modified Leibovitz's L15 medium and incubated at 32(°)C. Serial transfer of material from several primary cultures was maintained and has led to the isolation of young cell lines. A cell line (AmE-711) has been established that is composed mainly of fibroblast-type cells that form an adherent monolayer. Most cells in the line are diploid (2n = 32) and have the Apis mellifera karyotype as revealed by Giemsa stain. The partial sequence for the mitochondrial-encoded cytochrome c oxidase subunit I (Cox 1) gene in the cell line is identical to those from honey bee tissues and a consensus sequence for A. mellifera. The population doubling time is approximately 4 days. Importantly, the cell line is continuously subcultured every 10-14 days when split at a 1:3 ratio and is cryopreserved in liquid nitrogen. The cell culture system we have developed has potential application for studies aimed at honey bee development, genetics, pathogenesis, transgenesis, and toxicology.
format	article
author	Michael J Goblirsch Marla S Spivak Timothy J Kurtti
author_facet	Michael J Goblirsch Marla S Spivak Timothy J Kurtti
author_sort	Michael J Goblirsch
title	A cell line resource derived from honey bee (Apis mellifera) embryonic tissues.
title_short	A cell line resource derived from honey bee (Apis mellifera) embryonic tissues.
title_full	A cell line resource derived from honey bee (Apis mellifera) embryonic tissues.
title_fullStr	A cell line resource derived from honey bee (Apis mellifera) embryonic tissues.
title_full_unstemmed	A cell line resource derived from honey bee (Apis mellifera) embryonic tissues.
title_sort	cell line resource derived from honey bee (apis mellifera) embryonic tissues.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/cca8903f8d3d4e6989d72c9f22467b5d
work_keys_str_mv	AT michaeljgoblirsch acelllineresourcederivedfromhoneybeeapismelliferaembryonictissues AT marlasspivak acelllineresourcederivedfromhoneybeeapismelliferaembryonictissues AT timothyjkurtti acelllineresourcederivedfromhoneybeeapismelliferaembryonictissues AT michaeljgoblirsch celllineresourcederivedfromhoneybeeapismelliferaembryonictissues AT marlasspivak celllineresourcederivedfromhoneybeeapismelliferaembryonictissues AT timothyjkurtti celllineresourcederivedfromhoneybeeapismelliferaembryonictissues
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A cell line resource derived from honey bee (Apis mellifera) embryonic tissues.

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