Protective Effect of Hyperbaric Oxygen Treatment on Axon Degeneration after Acute Motor Axonal Neuropathy

Objectives. Acute motor axonal neuropathy (AMAN) is a disease that leads to acute flaccid paralysis and may result from the binding of antibody and antigen to the spinal cord. The objective of this study is to evaluate the protective effect of hyperbaric oxygen treatment (HBOT) on axon degeneration...

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Autores principales:	Ni Komang Sri Dewi Untari, Kurnia Kusumastuti, Guritno Suryokusumo, I Ketut Sudiana
Formato:	article
Lenguaje:	EN
Publicado:	Hindawi Limited 2021
Materias:	Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/ccb72cf3467944c49895bb9de2a24b16
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spelling	oai:doaj.org-article:ccb72cf3467944c49895bb9de2a24b162021-11-22T01:11:27ZProtective Effect of Hyperbaric Oxygen Treatment on Axon Degeneration after Acute Motor Axonal Neuropathy2090-043010.1155/2021/6627779https://doaj.org/article/ccb72cf3467944c49895bb9de2a24b162021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/6627779https://doaj.org/toc/2090-0430Objectives. Acute motor axonal neuropathy (AMAN) is a disease that leads to acute flaccid paralysis and may result from the binding of antibody and antigen to the spinal cord. The objective of this study is to evaluate the protective effect of hyperbaric oxygen treatment (HBOT) on axon degeneration of the spinal cord and sciatic nerve of the AMAN model rabbit. Axonal degeneration was assessed by evaluating glutathione (GSH) activity, interleukin-1β (IL-1β) expression, and clinical and histopathological features. Methods. Twenty-one New Zealand rabbits were divided into three groups. The treatment group was exposed to 100% oxygen at 2.4 ATA 90 minutes for 10 days at a decompression rate of 2.9 pounds per square inch/minute. GSH level was evaluated using an enzyme-linked immune-sorbent assay. An expression of IL-1β in the spinal cord was determined by immunohistochemistry. Clinical appearances were done by motor scale and body weight. Histological features observed neuronal swelling and inflammatory infiltration in the sagittal lumbar region and the undulation of the longitudinal sciatic nerve. Results. Rabbits exposed to HBO had high GSH activity levels (p<0.05) but unexpectedly had high IL1β expression (p>0.05). In addition, the HBO-exposed rabbits had a better degree of undulation, the size of neuronal swelling was smaller, the number of macrophages was higher, and motor function was better than the AMAN model rabbits (p<0.05). Conclusions. These findings indicate that HBO therapy can decrease axon degeneration by triggering GSH activity, increasing IL-1β level, and restoring tissues and motor status. In conclusion, HBO has a protective effect on axon degeneration of the spinal cord and sciatic nerve of the AMAN model rabbit.Ni Komang Sri Dewi UntariKurnia KusumastutiGuritno SuryokusumoI Ketut SudianaHindawi LimitedarticleImmunologic diseases. AllergyRC581-607ENAutoimmune Diseases, Vol 2021 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607
spellingShingle	Immunologic diseases. Allergy RC581-607 Ni Komang Sri Dewi Untari Kurnia Kusumastuti Guritno Suryokusumo I Ketut Sudiana Protective Effect of Hyperbaric Oxygen Treatment on Axon Degeneration after Acute Motor Axonal Neuropathy
description	Objectives. Acute motor axonal neuropathy (AMAN) is a disease that leads to acute flaccid paralysis and may result from the binding of antibody and antigen to the spinal cord. The objective of this study is to evaluate the protective effect of hyperbaric oxygen treatment (HBOT) on axon degeneration of the spinal cord and sciatic nerve of the AMAN model rabbit. Axonal degeneration was assessed by evaluating glutathione (GSH) activity, interleukin-1β (IL-1β) expression, and clinical and histopathological features. Methods. Twenty-one New Zealand rabbits were divided into three groups. The treatment group was exposed to 100% oxygen at 2.4 ATA 90 minutes for 10 days at a decompression rate of 2.9 pounds per square inch/minute. GSH level was evaluated using an enzyme-linked immune-sorbent assay. An expression of IL-1β in the spinal cord was determined by immunohistochemistry. Clinical appearances were done by motor scale and body weight. Histological features observed neuronal swelling and inflammatory infiltration in the sagittal lumbar region and the undulation of the longitudinal sciatic nerve. Results. Rabbits exposed to HBO had high GSH activity levels (p<0.05) but unexpectedly had high IL1β expression (p>0.05). In addition, the HBO-exposed rabbits had a better degree of undulation, the size of neuronal swelling was smaller, the number of macrophages was higher, and motor function was better than the AMAN model rabbits (p<0.05). Conclusions. These findings indicate that HBO therapy can decrease axon degeneration by triggering GSH activity, increasing IL-1β level, and restoring tissues and motor status. In conclusion, HBO has a protective effect on axon degeneration of the spinal cord and sciatic nerve of the AMAN model rabbit.
format	article
author	Ni Komang Sri Dewi Untari Kurnia Kusumastuti Guritno Suryokusumo I Ketut Sudiana
author_facet	Ni Komang Sri Dewi Untari Kurnia Kusumastuti Guritno Suryokusumo I Ketut Sudiana
author_sort	Ni Komang Sri Dewi Untari
title	Protective Effect of Hyperbaric Oxygen Treatment on Axon Degeneration after Acute Motor Axonal Neuropathy
title_short	Protective Effect of Hyperbaric Oxygen Treatment on Axon Degeneration after Acute Motor Axonal Neuropathy
title_full	Protective Effect of Hyperbaric Oxygen Treatment on Axon Degeneration after Acute Motor Axonal Neuropathy
title_fullStr	Protective Effect of Hyperbaric Oxygen Treatment on Axon Degeneration after Acute Motor Axonal Neuropathy
title_full_unstemmed	Protective Effect of Hyperbaric Oxygen Treatment on Axon Degeneration after Acute Motor Axonal Neuropathy
title_sort	protective effect of hyperbaric oxygen treatment on axon degeneration after acute motor axonal neuropathy
publisher	Hindawi Limited
publishDate	2021
url	https://doaj.org/article/ccb72cf3467944c49895bb9de2a24b16
work_keys_str_mv	AT nikomangsridewiuntari protectiveeffectofhyperbaricoxygentreatmentonaxondegenerationafteracutemotoraxonalneuropathy AT kurniakusumastuti protectiveeffectofhyperbaricoxygentreatmentonaxondegenerationafteracutemotoraxonalneuropathy AT guritnosuryokusumo protectiveeffectofhyperbaricoxygentreatmentonaxondegenerationafteracutemotoraxonalneuropathy AT iketutsudiana protectiveeffectofhyperbaricoxygentreatmentonaxondegenerationafteracutemotoraxonalneuropathy
_version_	1718418307974955008

Protective Effect of Hyperbaric Oxygen Treatment on Axon Degeneration after Acute Motor Axonal Neuropathy

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