Cytokine Profiling in Low- and High-Density Small Extracellular Vesicles from Epidermoid Carcinoma Cells

Exosomes or small extracellular vesicles (sEVs) are membrane-bound nanoparticles that carry various macromolecules and act as autocrine and paracrine signaling messengers. In this study, sEVs from epidermoid carcinoma cells influenced by membrane presentation of the glycoprotein desmoglein 2 and its...

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Autores principales:	Joseph P. Flemming, Brianna L. Hill, Lauren Anderson-Pullinger, Larry A. Harshyne, Mỹ G. Mahoney
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Dermatology RL1-803
Acceso en línea:	https://doaj.org/article/ccc7c88ae91146c2a80eebbae2ebcc55
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spelling	oai:doaj.org-article:ccc7c88ae91146c2a80eebbae2ebcc552021-11-06T04:42:22ZCytokine Profiling in Low- and High-Density Small Extracellular Vesicles from Epidermoid Carcinoma Cells2667-026710.1016/j.xjidi.2021.100053https://doaj.org/article/ccc7c88ae91146c2a80eebbae2ebcc552021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2667026721000540https://doaj.org/toc/2667-0267Exosomes or small extracellular vesicles (sEVs) are membrane-bound nanoparticles that carry various macromolecules and act as autocrine and paracrine signaling messengers. In this study, sEVs from epidermoid carcinoma cells influenced by membrane presentation of the glycoprotein desmoglein 2 and its palmitoylation state were investigated. In this study, sEVs were isolated by sequential ultracentrifugation followed by iodixanol density gradient separation. They were then subjected to multiplex profiling of cytokines associated with the surface of intact sEVs. The results revealed a previously undescribed active sorting of cytokines onto the surface of low-density and high-density sEV subpopulations. Specifically, an altered surface presentation of desmoglein 2 decreased FGF-2 and VEGF in low-density sEVs. In addition, in response to desmoglein 2, IL-8 and RANTES were increased in low-density sEVs but only slightly decreased in high-density sEVs. Finally, IL-6 and G-CSF were increased dramatically in high-density sEVs. This comprehensive analysis of the cytokine production profile by squamous cell carcinoma‒derived sEVs highlights their contribution to immune evasion, pro-oncogenic and proangiogenic activity, and the potential to identify diagnostic disease biomarkers.Joseph P. FlemmingBrianna L. HillLauren Anderson-PullingerLarry A. HarshyneMỹ G. MahoneyElsevierarticleDermatologyRL1-803ENJID Innovations, Vol 1, Iss 4, Pp 100053- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Dermatology RL1-803
spellingShingle	Dermatology RL1-803 Joseph P. Flemming Brianna L. Hill Lauren Anderson-Pullinger Larry A. Harshyne Mỹ G. Mahoney Cytokine Profiling in Low- and High-Density Small Extracellular Vesicles from Epidermoid Carcinoma Cells
description	Exosomes or small extracellular vesicles (sEVs) are membrane-bound nanoparticles that carry various macromolecules and act as autocrine and paracrine signaling messengers. In this study, sEVs from epidermoid carcinoma cells influenced by membrane presentation of the glycoprotein desmoglein 2 and its palmitoylation state were investigated. In this study, sEVs were isolated by sequential ultracentrifugation followed by iodixanol density gradient separation. They were then subjected to multiplex profiling of cytokines associated with the surface of intact sEVs. The results revealed a previously undescribed active sorting of cytokines onto the surface of low-density and high-density sEV subpopulations. Specifically, an altered surface presentation of desmoglein 2 decreased FGF-2 and VEGF in low-density sEVs. In addition, in response to desmoglein 2, IL-8 and RANTES were increased in low-density sEVs but only slightly decreased in high-density sEVs. Finally, IL-6 and G-CSF were increased dramatically in high-density sEVs. This comprehensive analysis of the cytokine production profile by squamous cell carcinoma‒derived sEVs highlights their contribution to immune evasion, pro-oncogenic and proangiogenic activity, and the potential to identify diagnostic disease biomarkers.
format	article
author	Joseph P. Flemming Brianna L. Hill Lauren Anderson-Pullinger Larry A. Harshyne Mỹ G. Mahoney
author_facet	Joseph P. Flemming Brianna L. Hill Lauren Anderson-Pullinger Larry A. Harshyne Mỹ G. Mahoney
author_sort	Joseph P. Flemming
title	Cytokine Profiling in Low- and High-Density Small Extracellular Vesicles from Epidermoid Carcinoma Cells
title_short	Cytokine Profiling in Low- and High-Density Small Extracellular Vesicles from Epidermoid Carcinoma Cells
title_full	Cytokine Profiling in Low- and High-Density Small Extracellular Vesicles from Epidermoid Carcinoma Cells
title_fullStr	Cytokine Profiling in Low- and High-Density Small Extracellular Vesicles from Epidermoid Carcinoma Cells
title_full_unstemmed	Cytokine Profiling in Low- and High-Density Small Extracellular Vesicles from Epidermoid Carcinoma Cells
title_sort	cytokine profiling in low- and high-density small extracellular vesicles from epidermoid carcinoma cells
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/ccc7c88ae91146c2a80eebbae2ebcc55
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Cytokine Profiling in Low- and High-Density Small Extracellular Vesicles from Epidermoid Carcinoma Cells

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