Lactate regulates rat male germ cell function through reactive oxygen species.

Besides giving structural support, Sertoli cells regulate the fate of germ cells by supplying a variety of factors. These factors include hormones, several pro- and anti-apoptotic agents and also energetic substrates. Lactate is one of the compounds produced by Sertoli cells, which is utilized as an...

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Autores principales:	María Noel Galardo, Mariana Regueira, María Fernanda Riera, Eliana Herminia Pellizzari, Selva Beatriz Cigorraga, Silvina Beatriz Meroni
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ccd43d0eea1a461ca25d811dcb39396d
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spelling	oai:doaj.org-article:ccd43d0eea1a461ca25d811dcb39396d2021-11-18T08:34:24ZLactate regulates rat male germ cell function through reactive oxygen species.1932-620310.1371/journal.pone.0088024https://doaj.org/article/ccd43d0eea1a461ca25d811dcb39396d2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24498241/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Besides giving structural support, Sertoli cells regulate the fate of germ cells by supplying a variety of factors. These factors include hormones, several pro- and anti-apoptotic agents and also energetic substrates. Lactate is one of the compounds produced by Sertoli cells, which is utilized as an energetic substrate by germ cells, particularly spermatocytes and spermatids. Beyond its function as an energy source, some studies have proposed a role of lactate in the regulation of gene expression not strictly related to the energetic state of the cells. The general hypothesis that motivated this investigation was that lactate affects male germ cell function, far beyond its well-known role as energetic substrate. To evaluate this hypothesis we investigated: 1) if lactate was able to regulate germ cell gene expression and if reactive oxygen species (ROS) participated in this regulation, 2) if different signal transduction pathways were modified by the production of ROS in response to lactate and 3) possible mechanisms that may be involved in lactate stimulation of ROS production. In order to achieve these goals, cultures of germ cells obtained from male 30-day old rats were exposed to 10 or 20 mM lactate. Increases in lactate dehydrogenase (LDH) C and monocarboxylate transporter (MCT)2 expression, in Akt and p38-MAPK phosphorylation levels and in ROS production were observed. These effects were impaired in the presence of a ROS scavenger. Lactate stimulated ROS production was also inhibited by a LDH inhibitor or a NAD(P)H oxidase (NOX) inhibitor. NOX4 expression was identified in male germ cells. The results obtained herein are consistent with a scenario where lactate, taken up by germ cells, becomes oxidized to pyruvate with the resultant increase in NADH, which is a substrate for NOX4. ROS, products of NOX4 activity, may act as second messengers regulating signal transduction pathways and gene expression.María Noel GalardoMariana RegueiraMaría Fernanda RieraEliana Herminia PellizzariSelva Beatriz CigorragaSilvina Beatriz MeroniPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 1, p e88024 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q María Noel Galardo Mariana Regueira María Fernanda Riera Eliana Herminia Pellizzari Selva Beatriz Cigorraga Silvina Beatriz Meroni Lactate regulates rat male germ cell function through reactive oxygen species.
description	Besides giving structural support, Sertoli cells regulate the fate of germ cells by supplying a variety of factors. These factors include hormones, several pro- and anti-apoptotic agents and also energetic substrates. Lactate is one of the compounds produced by Sertoli cells, which is utilized as an energetic substrate by germ cells, particularly spermatocytes and spermatids. Beyond its function as an energy source, some studies have proposed a role of lactate in the regulation of gene expression not strictly related to the energetic state of the cells. The general hypothesis that motivated this investigation was that lactate affects male germ cell function, far beyond its well-known role as energetic substrate. To evaluate this hypothesis we investigated: 1) if lactate was able to regulate germ cell gene expression and if reactive oxygen species (ROS) participated in this regulation, 2) if different signal transduction pathways were modified by the production of ROS in response to lactate and 3) possible mechanisms that may be involved in lactate stimulation of ROS production. In order to achieve these goals, cultures of germ cells obtained from male 30-day old rats were exposed to 10 or 20 mM lactate. Increases in lactate dehydrogenase (LDH) C and monocarboxylate transporter (MCT)2 expression, in Akt and p38-MAPK phosphorylation levels and in ROS production were observed. These effects were impaired in the presence of a ROS scavenger. Lactate stimulated ROS production was also inhibited by a LDH inhibitor or a NAD(P)H oxidase (NOX) inhibitor. NOX4 expression was identified in male germ cells. The results obtained herein are consistent with a scenario where lactate, taken up by germ cells, becomes oxidized to pyruvate with the resultant increase in NADH, which is a substrate for NOX4. ROS, products of NOX4 activity, may act as second messengers regulating signal transduction pathways and gene expression.
format	article
author	María Noel Galardo Mariana Regueira María Fernanda Riera Eliana Herminia Pellizzari Selva Beatriz Cigorraga Silvina Beatriz Meroni
author_facet	María Noel Galardo Mariana Regueira María Fernanda Riera Eliana Herminia Pellizzari Selva Beatriz Cigorraga Silvina Beatriz Meroni
author_sort	María Noel Galardo
title	Lactate regulates rat male germ cell function through reactive oxygen species.
title_short	Lactate regulates rat male germ cell function through reactive oxygen species.
title_full	Lactate regulates rat male germ cell function through reactive oxygen species.
title_fullStr	Lactate regulates rat male germ cell function through reactive oxygen species.
title_full_unstemmed	Lactate regulates rat male germ cell function through reactive oxygen species.
title_sort	lactate regulates rat male germ cell function through reactive oxygen species.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/ccd43d0eea1a461ca25d811dcb39396d
work_keys_str_mv	AT marianoelgalardo lactateregulatesratmalegermcellfunctionthroughreactiveoxygenspecies AT marianaregueira lactateregulatesratmalegermcellfunctionthroughreactiveoxygenspecies AT mariafernandariera lactateregulatesratmalegermcellfunctionthroughreactiveoxygenspecies AT elianaherminiapellizzari lactateregulatesratmalegermcellfunctionthroughreactiveoxygenspecies AT selvabeatrizcigorraga lactateregulatesratmalegermcellfunctionthroughreactiveoxygenspecies AT silvinabeatrizmeroni lactateregulatesratmalegermcellfunctionthroughreactiveoxygenspecies
_version_	1718421648686710784

Lactate regulates rat male germ cell function through reactive oxygen species.

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