Visualisation of dCas9 target search in vivo using an open-microscopy framework

Single-particle tracking PALM (sptPALM) provides quantitative information in vivo if the protein of interest remains in a single diffusional state during track acquisition. Here the authors develop a custom-built sptPALM microscope and a Monte-Carlo based diffusion distribution analysis to study dyn...

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Autores principales:	Koen J. A. Martens, Sam P. B. van Beljouw, Simon van der Els, Jochem N. A. Vink, Sander Baas, George A. Vogelaar, Stan J. J. Brouns, Peter van Baarlen, Michiel Kleerebezem, Johannes Hohlbein
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/cce2b1746abc4b5e80be17c038eef2ce
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spelling	oai:doaj.org-article:cce2b1746abc4b5e80be17c038eef2ce2021-12-02T17:02:12ZVisualisation of dCas9 target search in vivo using an open-microscopy framework10.1038/s41467-019-11514-02041-1723https://doaj.org/article/cce2b1746abc4b5e80be17c038eef2ce2019-08-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-11514-0https://doaj.org/toc/2041-1723Single-particle tracking PALM (sptPALM) provides quantitative information in vivo if the protein of interest remains in a single diffusional state during track acquisition. Here the authors develop a custom-built sptPALM microscope and a Monte-Carlo based diffusion distribution analysis to study dynamic DNA-dCas9 interactions in live bacteria.Koen J. A. MartensSam P. B. van BeljouwSimon van der ElsJochem N. A. VinkSander BaasGeorge A. VogelaarStan J. J. BrounsPeter van BaarlenMichiel KleerebezemJohannes HohlbeinNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-11 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Koen J. A. Martens Sam P. B. van Beljouw Simon van der Els Jochem N. A. Vink Sander Baas George A. Vogelaar Stan J. J. Brouns Peter van Baarlen Michiel Kleerebezem Johannes Hohlbein Visualisation of dCas9 target search in vivo using an open-microscopy framework
description	Single-particle tracking PALM (sptPALM) provides quantitative information in vivo if the protein of interest remains in a single diffusional state during track acquisition. Here the authors develop a custom-built sptPALM microscope and a Monte-Carlo based diffusion distribution analysis to study dynamic DNA-dCas9 interactions in live bacteria.
format	article
author	Koen J. A. Martens Sam P. B. van Beljouw Simon van der Els Jochem N. A. Vink Sander Baas George A. Vogelaar Stan J. J. Brouns Peter van Baarlen Michiel Kleerebezem Johannes Hohlbein
author_facet	Koen J. A. Martens Sam P. B. van Beljouw Simon van der Els Jochem N. A. Vink Sander Baas George A. Vogelaar Stan J. J. Brouns Peter van Baarlen Michiel Kleerebezem Johannes Hohlbein
author_sort	Koen J. A. Martens
title	Visualisation of dCas9 target search in vivo using an open-microscopy framework
title_short	Visualisation of dCas9 target search in vivo using an open-microscopy framework
title_full	Visualisation of dCas9 target search in vivo using an open-microscopy framework
title_fullStr	Visualisation of dCas9 target search in vivo using an open-microscopy framework
title_full_unstemmed	Visualisation of dCas9 target search in vivo using an open-microscopy framework
title_sort	visualisation of dcas9 target search in vivo using an open-microscopy framework
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/cce2b1746abc4b5e80be17c038eef2ce
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Visualisation of dCas9 target search in vivo using an open-microscopy framework

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