Visualisation of dCas9 target search in vivo using an open-microscopy framework

Visualisation of dCas9 target search in vivo using an open-microscopy framework

Single-particle tracking PALM (sptPALM) provides quantitative information in vivo if the protein of interest remains in a single diffusional state during track acquisition. Here the authors develop a custom-built sptPALM microscope and a Monte-Carlo based diffusion distribution analysis to study dyn...

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Autores principales: Koen J. A. Martens, Sam P. B. van Beljouw, Simon van der Els, Jochem N. A. Vink, Sander Baas, George A. Vogelaar, Stan J. J. Brouns, Peter van Baarlen, Michiel Kleerebezem, Johannes Hohlbein
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2019
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Science
Q
Acceso en línea:https://doaj.org/article/cce2b1746abc4b5e80be17c038eef2ce
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