How gastrin-releasing peptide receptor (GRPR) and αvβ3 integrin expression reflect reorganization features of tumors after hyperthermia treatments

Abstract The outcome of tumor treatment via hyperthermia in the clinic has been reported to be heterogeneous. Here, we assessed how the presence of gastrin-releasing peptide receptor (GRPR) and αvβ3 integrin together with the morphology of the vascularization reflects the growth behavior of tumors a...

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Autores principales:	Sandra Hallasch, Sindy Frick, Maximilian Jung, Ingrid Hilger
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cce439f8c0b4449f8441f3fc12481bc8
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spelling	oai:doaj.org-article:cce439f8c0b4449f8441f3fc12481bc82021-12-02T11:52:59ZHow gastrin-releasing peptide receptor (GRPR) and αvβ3 integrin expression reflect reorganization features of tumors after hyperthermia treatments10.1038/s41598-017-06100-72045-2322https://doaj.org/article/cce439f8c0b4449f8441f3fc12481bc82017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06100-7https://doaj.org/toc/2045-2322Abstract The outcome of tumor treatment via hyperthermia in the clinic has been reported to be heterogeneous. Here, we assessed how the presence of gastrin-releasing peptide receptor (GRPR) and αvβ3 integrin together with the morphology of the vascularization reflects the growth behavior of tumors after hyperthermia treatment. MDA-MB-231 tumor bearing mice were treated either with high (46 °C) or low dose (42 °C) water hyperthermia for 60 min. Changes of GRPR and αvβ3 integrin expression were assessed via multiplexed optical imaging. Vascularization was reconstructed and quantified by µCT imaging after contrast agent injection. We found that high dose hyperthermia is capable of increasing the expression of GRPR, αvβ3 integrin, CD31, and Ki67 in tumors. Also the morphology of tumor vasculature changed (increased relative blood volume and small-diameter vessel density, decreased expression of α-SMA). Low dose hyperthermia induced comparatively moderate effects on the investigated protein expression pattern and vascular remodeling. We conclude that under defined circumstances, specific temperature doses affect the reorganization of tumor regrowth, which is triggered by residual “dormant” cells even though tumor volumes are transiently decreasing. Further on, GRPR, αvβ3 integrin expression are versatile tools to surveil potential tumor regrow during therapy, beyond the conventional determination of tumor volumes.Sandra HallaschSindy FrickMaximilian JungIngrid HilgerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sandra Hallasch Sindy Frick Maximilian Jung Ingrid Hilger How gastrin-releasing peptide receptor (GRPR) and αvβ3 integrin expression reflect reorganization features of tumors after hyperthermia treatments
description	Abstract The outcome of tumor treatment via hyperthermia in the clinic has been reported to be heterogeneous. Here, we assessed how the presence of gastrin-releasing peptide receptor (GRPR) and αvβ3 integrin together with the morphology of the vascularization reflects the growth behavior of tumors after hyperthermia treatment. MDA-MB-231 tumor bearing mice were treated either with high (46 °C) or low dose (42 °C) water hyperthermia for 60 min. Changes of GRPR and αvβ3 integrin expression were assessed via multiplexed optical imaging. Vascularization was reconstructed and quantified by µCT imaging after contrast agent injection. We found that high dose hyperthermia is capable of increasing the expression of GRPR, αvβ3 integrin, CD31, and Ki67 in tumors. Also the morphology of tumor vasculature changed (increased relative blood volume and small-diameter vessel density, decreased expression of α-SMA). Low dose hyperthermia induced comparatively moderate effects on the investigated protein expression pattern and vascular remodeling. We conclude that under defined circumstances, specific temperature doses affect the reorganization of tumor regrowth, which is triggered by residual “dormant” cells even though tumor volumes are transiently decreasing. Further on, GRPR, αvβ3 integrin expression are versatile tools to surveil potential tumor regrow during therapy, beyond the conventional determination of tumor volumes.
format	article
author	Sandra Hallasch Sindy Frick Maximilian Jung Ingrid Hilger
author_facet	Sandra Hallasch Sindy Frick Maximilian Jung Ingrid Hilger
author_sort	Sandra Hallasch
title	How gastrin-releasing peptide receptor (GRPR) and αvβ3 integrin expression reflect reorganization features of tumors after hyperthermia treatments
title_short	How gastrin-releasing peptide receptor (GRPR) and αvβ3 integrin expression reflect reorganization features of tumors after hyperthermia treatments
title_full	How gastrin-releasing peptide receptor (GRPR) and αvβ3 integrin expression reflect reorganization features of tumors after hyperthermia treatments
title_fullStr	How gastrin-releasing peptide receptor (GRPR) and αvβ3 integrin expression reflect reorganization features of tumors after hyperthermia treatments
title_full_unstemmed	How gastrin-releasing peptide receptor (GRPR) and αvβ3 integrin expression reflect reorganization features of tumors after hyperthermia treatments
title_sort	how gastrin-releasing peptide receptor (grpr) and αvβ3 integrin expression reflect reorganization features of tumors after hyperthermia treatments
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/cce439f8c0b4449f8441f3fc12481bc8
work_keys_str_mv	AT sandrahallasch howgastrinreleasingpeptidereceptorgrprandavb3integrinexpressionreflectreorganizationfeaturesoftumorsafterhyperthermiatreatments AT sindyfrick howgastrinreleasingpeptidereceptorgrprandavb3integrinexpressionreflectreorganizationfeaturesoftumorsafterhyperthermiatreatments AT maximilianjung howgastrinreleasingpeptidereceptorgrprandavb3integrinexpressionreflectreorganizationfeaturesoftumorsafterhyperthermiatreatments AT ingridhilger howgastrinreleasingpeptidereceptorgrprandavb3integrinexpressionreflectreorganizationfeaturesoftumorsafterhyperthermiatreatments
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How gastrin-releasing peptide receptor (GRPR) and αvβ3 integrin expression reflect reorganization features of tumors after hyperthermia treatments

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