Geometrical microfeature cues for directing tubulogenesis of endothelial cells.

Angiogenesis, the formation of new blood vessels by sprouting from pre-existing ones, is critical for the establishment and maintenance of complex tissues. Angiogenesis is usually triggered by soluble growth factors such as VEGF. However, geometrical cues also play an important role in this process....

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Autores principales:	Yifeng Lei, Omar F Zouani, Murielle Rémy, Cédric Ayela, Marie-Christine Durrieu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cce4bd7a36ba49989ffcc2e1d37a53c7
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spelling	oai:doaj.org-article:cce4bd7a36ba49989ffcc2e1d37a53c72021-11-18T07:11:50ZGeometrical microfeature cues for directing tubulogenesis of endothelial cells.1932-620310.1371/journal.pone.0041163https://doaj.org/article/cce4bd7a36ba49989ffcc2e1d37a53c72012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22829923/?tool=EBIhttps://doaj.org/toc/1932-6203Angiogenesis, the formation of new blood vessels by sprouting from pre-existing ones, is critical for the establishment and maintenance of complex tissues. Angiogenesis is usually triggered by soluble growth factors such as VEGF. However, geometrical cues also play an important role in this process. Here we report the induction of angiogenesis solely by SVVYGLR peptide micropatterning on polymer surfaces. SVVYGLR peptide stripes were micropatterned onto polymer surfaces by photolithography to study their effects on endothelial cell (EC) behavior. Our results showed that the EC behaviors (cell spreading, orientation and migration) were significantly more guided and regulated on narrower SVVYGLR micropatterns (10 and 50 µm) than on larger stripes (100 µm). Also, EC morphogenesis into tube formation was switched on onto the smaller patterns. We illustrated that the central lumen of tubular structures can be formed by only one-to-four cells due to geometrical constraints on the micropatterns which mediated cell-substrate adhesion and generated a correct maturation of adherens junctions. In addition, sprouting of ECs and vascular networks were also induced by geometrical cues on surfaces micropatterned with SVVYGLR peptides. These micropatterned surfaces provide opportunities for mimicking angiogenesis by peptide modification instead of exogenous growth factors. The organization of ECs into tubular structures and the induction of sprouting angiogenesis are important towards the fabrication of vascularized tissues, and this work has great potential applications in tissue engineering and tissue regeneration.Yifeng LeiOmar F ZouaniMurielle RémyCédric AyelaMarie-Christine DurrieuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 7, p e41163 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yifeng Lei Omar F Zouani Murielle Rémy Cédric Ayela Marie-Christine Durrieu Geometrical microfeature cues for directing tubulogenesis of endothelial cells.
description	Angiogenesis, the formation of new blood vessels by sprouting from pre-existing ones, is critical for the establishment and maintenance of complex tissues. Angiogenesis is usually triggered by soluble growth factors such as VEGF. However, geometrical cues also play an important role in this process. Here we report the induction of angiogenesis solely by SVVYGLR peptide micropatterning on polymer surfaces. SVVYGLR peptide stripes were micropatterned onto polymer surfaces by photolithography to study their effects on endothelial cell (EC) behavior. Our results showed that the EC behaviors (cell spreading, orientation and migration) were significantly more guided and regulated on narrower SVVYGLR micropatterns (10 and 50 µm) than on larger stripes (100 µm). Also, EC morphogenesis into tube formation was switched on onto the smaller patterns. We illustrated that the central lumen of tubular structures can be formed by only one-to-four cells due to geometrical constraints on the micropatterns which mediated cell-substrate adhesion and generated a correct maturation of adherens junctions. In addition, sprouting of ECs and vascular networks were also induced by geometrical cues on surfaces micropatterned with SVVYGLR peptides. These micropatterned surfaces provide opportunities for mimicking angiogenesis by peptide modification instead of exogenous growth factors. The organization of ECs into tubular structures and the induction of sprouting angiogenesis are important towards the fabrication of vascularized tissues, and this work has great potential applications in tissue engineering and tissue regeneration.
format	article
author	Yifeng Lei Omar F Zouani Murielle Rémy Cédric Ayela Marie-Christine Durrieu
author_facet	Yifeng Lei Omar F Zouani Murielle Rémy Cédric Ayela Marie-Christine Durrieu
author_sort	Yifeng Lei
title	Geometrical microfeature cues for directing tubulogenesis of endothelial cells.
title_short	Geometrical microfeature cues for directing tubulogenesis of endothelial cells.
title_full	Geometrical microfeature cues for directing tubulogenesis of endothelial cells.
title_fullStr	Geometrical microfeature cues for directing tubulogenesis of endothelial cells.
title_full_unstemmed	Geometrical microfeature cues for directing tubulogenesis of endothelial cells.
title_sort	geometrical microfeature cues for directing tubulogenesis of endothelial cells.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/cce4bd7a36ba49989ffcc2e1d37a53c7
work_keys_str_mv	AT yifenglei geometricalmicrofeaturecuesfordirectingtubulogenesisofendothelialcells AT omarfzouani geometricalmicrofeaturecuesfordirectingtubulogenesisofendothelialcells AT murielleremy geometricalmicrofeaturecuesfordirectingtubulogenesisofendothelialcells AT cedricayela geometricalmicrofeaturecuesfordirectingtubulogenesisofendothelialcells AT mariechristinedurrieu geometricalmicrofeaturecuesfordirectingtubulogenesisofendothelialcells
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Geometrical microfeature cues for directing tubulogenesis of endothelial cells.

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