C. elegans-based chemosensation strategy for the early detection of cancer metabolites in urine samples

Abstract Chemosensory receptors play a crucial role in distinguishing the wide range of volatile/soluble molecules by binding them with high accuracy. Chemosensation is the main sensory modality in organisms lacking long-range sensory mechanisms like vision/hearing. Despite its low number of sensory...

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Autores principales:	Enrico Lanza, Martina Di Rocco, Silvia Schwartz, Davide Caprini, Edoardo Milanetti, Giuseppe Ferrarese, Maria Teresa Lonardo, Luca Pannone, Giancarlo Ruocco, Simone Martinelli, Viola Folli
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cce7c2894e8d4308a4f73ba23829bbfc
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spelling	oai:doaj.org-article:cce7c2894e8d4308a4f73ba23829bbfc2021-12-02T15:09:07ZC. elegans-based chemosensation strategy for the early detection of cancer metabolites in urine samples10.1038/s41598-021-96613-z2045-2322https://doaj.org/article/cce7c2894e8d4308a4f73ba23829bbfc2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96613-zhttps://doaj.org/toc/2045-2322Abstract Chemosensory receptors play a crucial role in distinguishing the wide range of volatile/soluble molecules by binding them with high accuracy. Chemosensation is the main sensory modality in organisms lacking long-range sensory mechanisms like vision/hearing. Despite its low number of sensory neurons, the nematode Caenorhabditis elegans possesses several chemosensory receptors, allowing it to detect about as many odorants as mammals. Here, we show that C. elegans displays attraction towards urine samples of women with breast cancer, avoiding control ones. Behavioral assays on animals lacking AWC sensory neurons demonstrate the relevance of these neurons in sensing cancer odorants: calcium imaging on AWC increases the accuracy of the discrimination (97.22%). Also, chemotaxis assays on animals lacking GPCRs expressed in AWC allow to identify receptors involved in binding cancer metabolites, suggesting that an alteration of a few metabolites is sufficient for the cancer discriminating behavior of C. elegans, which may help identify a fundamental fingerprint of breast cancer.Enrico LanzaMartina Di RoccoSilvia SchwartzDavide CapriniEdoardo MilanettiGiuseppe FerrareseMaria Teresa LonardoLuca PannoneGiancarlo RuoccoSimone MartinelliViola FolliNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Enrico Lanza Martina Di Rocco Silvia Schwartz Davide Caprini Edoardo Milanetti Giuseppe Ferrarese Maria Teresa Lonardo Luca Pannone Giancarlo Ruocco Simone Martinelli Viola Folli C. elegans-based chemosensation strategy for the early detection of cancer metabolites in urine samples
description	Abstract Chemosensory receptors play a crucial role in distinguishing the wide range of volatile/soluble molecules by binding them with high accuracy. Chemosensation is the main sensory modality in organisms lacking long-range sensory mechanisms like vision/hearing. Despite its low number of sensory neurons, the nematode Caenorhabditis elegans possesses several chemosensory receptors, allowing it to detect about as many odorants as mammals. Here, we show that C. elegans displays attraction towards urine samples of women with breast cancer, avoiding control ones. Behavioral assays on animals lacking AWC sensory neurons demonstrate the relevance of these neurons in sensing cancer odorants: calcium imaging on AWC increases the accuracy of the discrimination (97.22%). Also, chemotaxis assays on animals lacking GPCRs expressed in AWC allow to identify receptors involved in binding cancer metabolites, suggesting that an alteration of a few metabolites is sufficient for the cancer discriminating behavior of C. elegans, which may help identify a fundamental fingerprint of breast cancer.
format	article
author	Enrico Lanza Martina Di Rocco Silvia Schwartz Davide Caprini Edoardo Milanetti Giuseppe Ferrarese Maria Teresa Lonardo Luca Pannone Giancarlo Ruocco Simone Martinelli Viola Folli
author_facet	Enrico Lanza Martina Di Rocco Silvia Schwartz Davide Caprini Edoardo Milanetti Giuseppe Ferrarese Maria Teresa Lonardo Luca Pannone Giancarlo Ruocco Simone Martinelli Viola Folli
author_sort	Enrico Lanza
title	C. elegans-based chemosensation strategy for the early detection of cancer metabolites in urine samples
title_short	C. elegans-based chemosensation strategy for the early detection of cancer metabolites in urine samples
title_full	C. elegans-based chemosensation strategy for the early detection of cancer metabolites in urine samples
title_fullStr	C. elegans-based chemosensation strategy for the early detection of cancer metabolites in urine samples
title_full_unstemmed	C. elegans-based chemosensation strategy for the early detection of cancer metabolites in urine samples
title_sort	c. elegans-based chemosensation strategy for the early detection of cancer metabolites in urine samples
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/cce7c2894e8d4308a4f73ba23829bbfc
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C. elegans-based chemosensation strategy for the early detection of cancer metabolites in urine samples

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