Evaluation of inflammation and follicle depletion during ovarian ageing in mice

Abstract Reproductive ageing in females is defined by a progressive decline in follicle number and oocyte quality. This is a natural process that leads to the loss of fertility and ovarian function, cycle irregularity and eventually menopause or reproductive senescence. The factors that underlie the...

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Autores principales:	Carolina Lliberos, Seng H. Liew, Pirooz Zareie, Nicole L. La Gruta, Ashley Mansell, Karla Hutt
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ccf2ef3a52fb47d880669b6ed595328e
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spelling	oai:doaj.org-article:ccf2ef3a52fb47d880669b6ed595328e2021-12-02T14:12:45ZEvaluation of inflammation and follicle depletion during ovarian ageing in mice10.1038/s41598-020-79488-42045-2322https://doaj.org/article/ccf2ef3a52fb47d880669b6ed595328e2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79488-4https://doaj.org/toc/2045-2322Abstract Reproductive ageing in females is defined by a progressive decline in follicle number and oocyte quality. This is a natural process that leads to the loss of fertility and ovarian function, cycle irregularity and eventually menopause or reproductive senescence. The factors that underlie the natural depletion of follicles throughout reproductive life are poorly characterised. It has been proposed that inflammatory processes and fibrosis might contribute to ovarian ageing. To further investigate this possibility, we evaluated key markers of inflammation and immune cell populations in the ovaries of 2, 6, 12 and 18-month-old C57BL/6 female mice. We report that the decrease in follicle numbers over the reproductive lifespan was associated with an increase in the intra-ovarian percentage of CD4 + T cells, B cells and macrophages. Serum concentration and intra-ovarian mRNA levels of several pro-inflammatory cytokines, including IL-1α/β, TNF-α, IL-6, and inflammasome genes ASC and NLRP3, were significantly increased with age. Fibrosis levels, as determined by picrosirius red staining for collagen I and III, were unchanged up to 18 months of age. Collectively, these data suggest that inflammation could be one of the mechanisms responsible for the age-related regulation of follicle number, but the role of fibrosis is unclear. Further studies are now required to determine if there is a causative relationship between inflammation and follicle depletion as females age.Carolina LliberosSeng H. LiewPirooz ZareieNicole L. La GrutaAshley MansellKarla HuttNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Carolina Lliberos Seng H. Liew Pirooz Zareie Nicole L. La Gruta Ashley Mansell Karla Hutt Evaluation of inflammation and follicle depletion during ovarian ageing in mice
description	Abstract Reproductive ageing in females is defined by a progressive decline in follicle number and oocyte quality. This is a natural process that leads to the loss of fertility and ovarian function, cycle irregularity and eventually menopause or reproductive senescence. The factors that underlie the natural depletion of follicles throughout reproductive life are poorly characterised. It has been proposed that inflammatory processes and fibrosis might contribute to ovarian ageing. To further investigate this possibility, we evaluated key markers of inflammation and immune cell populations in the ovaries of 2, 6, 12 and 18-month-old C57BL/6 female mice. We report that the decrease in follicle numbers over the reproductive lifespan was associated with an increase in the intra-ovarian percentage of CD4 + T cells, B cells and macrophages. Serum concentration and intra-ovarian mRNA levels of several pro-inflammatory cytokines, including IL-1α/β, TNF-α, IL-6, and inflammasome genes ASC and NLRP3, were significantly increased with age. Fibrosis levels, as determined by picrosirius red staining for collagen I and III, were unchanged up to 18 months of age. Collectively, these data suggest that inflammation could be one of the mechanisms responsible for the age-related regulation of follicle number, but the role of fibrosis is unclear. Further studies are now required to determine if there is a causative relationship between inflammation and follicle depletion as females age.
format	article
author	Carolina Lliberos Seng H. Liew Pirooz Zareie Nicole L. La Gruta Ashley Mansell Karla Hutt
author_facet	Carolina Lliberos Seng H. Liew Pirooz Zareie Nicole L. La Gruta Ashley Mansell Karla Hutt
author_sort	Carolina Lliberos
title	Evaluation of inflammation and follicle depletion during ovarian ageing in mice
title_short	Evaluation of inflammation and follicle depletion during ovarian ageing in mice
title_full	Evaluation of inflammation and follicle depletion during ovarian ageing in mice
title_fullStr	Evaluation of inflammation and follicle depletion during ovarian ageing in mice
title_full_unstemmed	Evaluation of inflammation and follicle depletion during ovarian ageing in mice
title_sort	evaluation of inflammation and follicle depletion during ovarian ageing in mice
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/ccf2ef3a52fb47d880669b6ed595328e
work_keys_str_mv	AT carolinalliberos evaluationofinflammationandfollicledepletionduringovarianageinginmice AT senghliew evaluationofinflammationandfollicledepletionduringovarianageinginmice AT piroozzareie evaluationofinflammationandfollicledepletionduringovarianageinginmice AT nicolellagruta evaluationofinflammationandfollicledepletionduringovarianageinginmice AT ashleymansell evaluationofinflammationandfollicledepletionduringovarianageinginmice AT karlahutt evaluationofinflammationandfollicledepletionduringovarianageinginmice
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Evaluation of inflammation and follicle depletion during ovarian ageing in mice

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