Long term expansion profile of mesenchymal stromal cells at protein nanosheet-stabilised bioemulsions for next generation cell culture microcarriers

Tremendous progress in the identification, isolation and expansion of stem cells has allowed their application in regenerative medicine and tissue engineering, and their use as advanced in vitro models. As a result, stem cell manufacturing increasingly requires scale up, parallelisation and automati...

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Autores principales:	Lihui Peng, Julien E. Gautrot
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Mesenchymal stem cell Emulsion Protein nanosheet Microdroplet Microcarrier Cell manufacturing Medicine (General) R5-920 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/ccf6b5382226476ba1bad9e12d6637d9
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spelling	oai:doaj.org-article:ccf6b5382226476ba1bad9e12d6637d92021-11-20T05:12:45ZLong term expansion profile of mesenchymal stromal cells at protein nanosheet-stabilised bioemulsions for next generation cell culture microcarriers2590-006410.1016/j.mtbio.2021.100159https://doaj.org/article/ccf6b5382226476ba1bad9e12d6637d92021-09-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2590006421000673https://doaj.org/toc/2590-0064Tremendous progress in the identification, isolation and expansion of stem cells has allowed their application in regenerative medicine and tissue engineering, and their use as advanced in vitro models. As a result, stem cell manufacturing increasingly requires scale up, parallelisation and automation. However, solid substrates currently used for the culture of adherent cells are poorly adapted for such applications, owing to their difficult processing from cell products, relatively high costs and their typical reliance on difficult to recycle plastics and microplastics. In this work, we show that bioemulsions formed of microdroplets stabilised by protein nanosheets displaying strong interfacial mechanics are well-suited for the scale up of adherent stem cells such as mesenchymal stromal cells (MSCs). We demonstrate that, over multiple passages (up to passage 10), MSCs retain comparable phenotypes when cultured on such bioemulsions, solid microcarriers (Synthemax II) and classic 2D tissue culture polystyrene. Phenotyping (cell proliferation, morphometry, flow cytometry and differentiation assays) of MSCs cultured for multiple passages on these systems indicate that, although stemness is lost at late passages when cultured on these different substrates, stem cell phenotypes remained comparable between different culture conditions, at any given passage. Hence our study validates the use of bioemulsions for the long term expansion of adherent stem cells and paves the way to the design of novel 3D bioreactors based on microdroplet microcarriers.Lihui PengJulien E. GautrotElsevierarticleMesenchymal stem cellEmulsionProtein nanosheetMicrodropletMicrocarrierCell manufacturingMedicine (General)R5-920Biology (General)QH301-705.5ENMaterials Today Bio, Vol 12, Iss , Pp 100159- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Mesenchymal stem cell Emulsion Protein nanosheet Microdroplet Microcarrier Cell manufacturing Medicine (General) R5-920 Biology (General) QH301-705.5
spellingShingle	Mesenchymal stem cell Emulsion Protein nanosheet Microdroplet Microcarrier Cell manufacturing Medicine (General) R5-920 Biology (General) QH301-705.5 Lihui Peng Julien E. Gautrot Long term expansion profile of mesenchymal stromal cells at protein nanosheet-stabilised bioemulsions for next generation cell culture microcarriers
description	Tremendous progress in the identification, isolation and expansion of stem cells has allowed their application in regenerative medicine and tissue engineering, and their use as advanced in vitro models. As a result, stem cell manufacturing increasingly requires scale up, parallelisation and automation. However, solid substrates currently used for the culture of adherent cells are poorly adapted for such applications, owing to their difficult processing from cell products, relatively high costs and their typical reliance on difficult to recycle plastics and microplastics. In this work, we show that bioemulsions formed of microdroplets stabilised by protein nanosheets displaying strong interfacial mechanics are well-suited for the scale up of adherent stem cells such as mesenchymal stromal cells (MSCs). We demonstrate that, over multiple passages (up to passage 10), MSCs retain comparable phenotypes when cultured on such bioemulsions, solid microcarriers (Synthemax II) and classic 2D tissue culture polystyrene. Phenotyping (cell proliferation, morphometry, flow cytometry and differentiation assays) of MSCs cultured for multiple passages on these systems indicate that, although stemness is lost at late passages when cultured on these different substrates, stem cell phenotypes remained comparable between different culture conditions, at any given passage. Hence our study validates the use of bioemulsions for the long term expansion of adherent stem cells and paves the way to the design of novel 3D bioreactors based on microdroplet microcarriers.
format	article
author	Lihui Peng Julien E. Gautrot
author_facet	Lihui Peng Julien E. Gautrot
author_sort	Lihui Peng
title	Long term expansion profile of mesenchymal stromal cells at protein nanosheet-stabilised bioemulsions for next generation cell culture microcarriers
title_short	Long term expansion profile of mesenchymal stromal cells at protein nanosheet-stabilised bioemulsions for next generation cell culture microcarriers
title_full	Long term expansion profile of mesenchymal stromal cells at protein nanosheet-stabilised bioemulsions for next generation cell culture microcarriers
title_fullStr	Long term expansion profile of mesenchymal stromal cells at protein nanosheet-stabilised bioemulsions for next generation cell culture microcarriers
title_full_unstemmed	Long term expansion profile of mesenchymal stromal cells at protein nanosheet-stabilised bioemulsions for next generation cell culture microcarriers
title_sort	long term expansion profile of mesenchymal stromal cells at protein nanosheet-stabilised bioemulsions for next generation cell culture microcarriers
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/ccf6b5382226476ba1bad9e12d6637d9
work_keys_str_mv	AT lihuipeng longtermexpansionprofileofmesenchymalstromalcellsatproteinnanosheetstabilisedbioemulsionsfornextgenerationcellculturemicrocarriers AT julienegautrot longtermexpansionprofileofmesenchymalstromalcellsatproteinnanosheetstabilisedbioemulsionsfornextgenerationcellculturemicrocarriers
_version_	1718419568569876480

Long term expansion profile of mesenchymal stromal cells at protein nanosheet-stabilised bioemulsions for next generation cell culture microcarriers

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