Spray deposition of live cells throughout the electrospinning process produces nanofibrous three-dimensional tissue scaffolds

Justin T Seil, Thomas J WebsterLaboratories for Nanomedicine Research, School of Engineering, Brown University, Providence, RI, USAAbstract: Compared with traditional in-vitro cell culture materials, three-dimensional nanofibrous scaffolds provide a superior environment for promoting cell functions....

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Autores principales:	Seil J, Webster TJ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/ccfc5d480a494c14867fa9ac8105c720
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spelling	oai:doaj.org-article:ccfc5d480a494c14867fa9ac8105c7202021-12-02T01:13:34ZSpray deposition of live cells throughout the electrospinning process produces nanofibrous three-dimensional tissue scaffolds1176-91141178-2013https://doaj.org/article/ccfc5d480a494c14867fa9ac8105c7202011-05-01T00:00:00Zhttp://www.dovepress.com/spray-deposition-of-live-cells-throughout-the-electrospinning-process--a7533https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Justin T Seil, Thomas J WebsterLaboratories for Nanomedicine Research, School of Engineering, Brown University, Providence, RI, USAAbstract: Compared with traditional in-vitro cell culture materials, three-dimensional nanofibrous scaffolds provide a superior environment for promoting cell functions. Since nanofibrous scaffolds have nanometer pore sizes, cells are unable to penetrate on their own, so must be incorporated into the scaffold during fabrication to ensure proper cell distribution. In this study, biodegradable and cytocompatible poly(DL-lactide-co-glycolide) (PLGA) nanofibers were produced using an electrospinning process. As a model cell line, fibroblasts were periodically sprayed from a pump-action spray bottle onto the developing scaffold. The viability of cells before and after spraying, and also after incorporation into the scaffold, was compared. Results indicated that cell spraying and the scaffold fabrication process did not significantly reduce cell viability. These findings, thus, contribute to the understanding of how to produce more physiological relevant cell-seeded nanofibrous scaffolds, an important element for the future of nanotechnology and tissue engineering.Keywords: nanomaterials, tissue engineering, PLGA, nanotechnologySeil JWebster TJDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 1095-1099 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Seil J Webster TJ Spray deposition of live cells throughout the electrospinning process produces nanofibrous three-dimensional tissue scaffolds
description	Justin T Seil, Thomas J WebsterLaboratories for Nanomedicine Research, School of Engineering, Brown University, Providence, RI, USAAbstract: Compared with traditional in-vitro cell culture materials, three-dimensional nanofibrous scaffolds provide a superior environment for promoting cell functions. Since nanofibrous scaffolds have nanometer pore sizes, cells are unable to penetrate on their own, so must be incorporated into the scaffold during fabrication to ensure proper cell distribution. In this study, biodegradable and cytocompatible poly(DL-lactide-co-glycolide) (PLGA) nanofibers were produced using an electrospinning process. As a model cell line, fibroblasts were periodically sprayed from a pump-action spray bottle onto the developing scaffold. The viability of cells before and after spraying, and also after incorporation into the scaffold, was compared. Results indicated that cell spraying and the scaffold fabrication process did not significantly reduce cell viability. These findings, thus, contribute to the understanding of how to produce more physiological relevant cell-seeded nanofibrous scaffolds, an important element for the future of nanotechnology and tissue engineering.Keywords: nanomaterials, tissue engineering, PLGA, nanotechnology
format	article
author	Seil J Webster TJ
author_facet	Seil J Webster TJ
author_sort	Seil J
title	Spray deposition of live cells throughout the electrospinning process produces nanofibrous three-dimensional tissue scaffolds
title_short	Spray deposition of live cells throughout the electrospinning process produces nanofibrous three-dimensional tissue scaffolds
title_full	Spray deposition of live cells throughout the electrospinning process produces nanofibrous three-dimensional tissue scaffolds
title_fullStr	Spray deposition of live cells throughout the electrospinning process produces nanofibrous three-dimensional tissue scaffolds
title_full_unstemmed	Spray deposition of live cells throughout the electrospinning process produces nanofibrous three-dimensional tissue scaffolds
title_sort	spray deposition of live cells throughout the electrospinning process produces nanofibrous three-dimensional tissue scaffolds
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/ccfc5d480a494c14867fa9ac8105c720
work_keys_str_mv	AT seilj spraydepositionoflivecellsthroughouttheelectrospinningprocessproducesnanofibrousthreedimensionaltissuescaffolds AT webstertj spraydepositionoflivecellsthroughouttheelectrospinningprocessproducesnanofibrousthreedimensionaltissuescaffolds
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Spray deposition of live cells throughout the electrospinning process produces nanofibrous three-dimensional tissue scaffolds

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