Partial hepatectomy enhances the growth of CC531 rat colorectal cancer cells both in vitro and in vivo

Abstract Partial hepatectomy (PHx) is the gold standard for the treatment of colorectal cancer liver metastases. However, after removing a substantial amount of hepatic tissue, growth factors are released to induce liver regeneration, which may promote the proliferation of liver micrometastases or c...

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Autores principales:	Borja Herrero de la Parte, Mikel González-Arribas, Iñaki Diaz-Sanz, Teodoro Palomares, Ignacio García-Alonso
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ccfe7faab5314b76a554cf666ba2b7e3
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spelling	oai:doaj.org-article:ccfe7faab5314b76a554cf666ba2b7e32021-12-02T11:35:53ZPartial hepatectomy enhances the growth of CC531 rat colorectal cancer cells both in vitro and in vivo10.1038/s41598-021-85082-z2045-2322https://doaj.org/article/ccfe7faab5314b76a554cf666ba2b7e32021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85082-zhttps://doaj.org/toc/2045-2322Abstract Partial hepatectomy (PHx) is the gold standard for the treatment of colorectal cancer liver metastases. However, after removing a substantial amount of hepatic tissue, growth factors are released to induce liver regeneration, which may promote the proliferation of liver micrometastases or circulating tumour cells still present in the patient. The aim of this study is to assess the effect of PHx on the growth of liver metastases induced by intrasplenic cell inoculation as well as on in vitro proliferation of the same cancer cell line. Liver tumours were induced in 18 WAG/RijHsd male rats, by seeding 250,000 syngeneic colorectal cancer cells (CC531) into the spleen. The left lateral lobe of the liver was mobilized and in half of the animals it was removed to achieve a 40% hepatectomy. Twenty-eight days after tumour induction, the animals were sacrificed and the liver was removed and sliced to assess the relative tumour surface area (RTSA%). CC531 cells were cultured in presence of foetal calf serum, non-hepatectomised (NRS) or hepatectomized rat serum (HRS), and their proliferation rate at 24, 48, and 72 h was measured. RTSA% was significantly higher in animals which had undergone PHx than in the controls (non-hepatectomised) (46.98 ± 8.76% vs. 18.73 ± 5.65%; p < 0.05). Analysing each lobe separately, this difference in favour of hepatectomized animals was relevant and statistically significant in the paramedian and caudate lobes. But in the right lobe the difference was scarce and not significant. In vitro, 2.5% HRS achieved stronger proliferative rates than the control cultures (10% FCS) or their equivalent of NRS. In this experimental model, a parallelism has been shown between the effect of PHx on the growth of colorectal cancer cells in the liver and the effect of the serum on those cells in vitro.Borja Herrero de la ParteMikel González-ArribasIñaki Diaz-SanzTeodoro PalomaresIgnacio García-AlonsoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Borja Herrero de la Parte Mikel González-Arribas Iñaki Diaz-Sanz Teodoro Palomares Ignacio García-Alonso Partial hepatectomy enhances the growth of CC531 rat colorectal cancer cells both in vitro and in vivo
description	Abstract Partial hepatectomy (PHx) is the gold standard for the treatment of colorectal cancer liver metastases. However, after removing a substantial amount of hepatic tissue, growth factors are released to induce liver regeneration, which may promote the proliferation of liver micrometastases or circulating tumour cells still present in the patient. The aim of this study is to assess the effect of PHx on the growth of liver metastases induced by intrasplenic cell inoculation as well as on in vitro proliferation of the same cancer cell line. Liver tumours were induced in 18 WAG/RijHsd male rats, by seeding 250,000 syngeneic colorectal cancer cells (CC531) into the spleen. The left lateral lobe of the liver was mobilized and in half of the animals it was removed to achieve a 40% hepatectomy. Twenty-eight days after tumour induction, the animals were sacrificed and the liver was removed and sliced to assess the relative tumour surface area (RTSA%). CC531 cells were cultured in presence of foetal calf serum, non-hepatectomised (NRS) or hepatectomized rat serum (HRS), and their proliferation rate at 24, 48, and 72 h was measured. RTSA% was significantly higher in animals which had undergone PHx than in the controls (non-hepatectomised) (46.98 ± 8.76% vs. 18.73 ± 5.65%; p < 0.05). Analysing each lobe separately, this difference in favour of hepatectomized animals was relevant and statistically significant in the paramedian and caudate lobes. But in the right lobe the difference was scarce and not significant. In vitro, 2.5% HRS achieved stronger proliferative rates than the control cultures (10% FCS) or their equivalent of NRS. In this experimental model, a parallelism has been shown between the effect of PHx on the growth of colorectal cancer cells in the liver and the effect of the serum on those cells in vitro.
format	article
author	Borja Herrero de la Parte Mikel González-Arribas Iñaki Diaz-Sanz Teodoro Palomares Ignacio García-Alonso
author_facet	Borja Herrero de la Parte Mikel González-Arribas Iñaki Diaz-Sanz Teodoro Palomares Ignacio García-Alonso
author_sort	Borja Herrero de la Parte
title	Partial hepatectomy enhances the growth of CC531 rat colorectal cancer cells both in vitro and in vivo
title_short	Partial hepatectomy enhances the growth of CC531 rat colorectal cancer cells both in vitro and in vivo
title_full	Partial hepatectomy enhances the growth of CC531 rat colorectal cancer cells both in vitro and in vivo
title_fullStr	Partial hepatectomy enhances the growth of CC531 rat colorectal cancer cells both in vitro and in vivo
title_full_unstemmed	Partial hepatectomy enhances the growth of CC531 rat colorectal cancer cells both in vitro and in vivo
title_sort	partial hepatectomy enhances the growth of cc531 rat colorectal cancer cells both in vitro and in vivo
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/ccfe7faab5314b76a554cf666ba2b7e3
work_keys_str_mv	AT borjaherrerodelaparte partialhepatectomyenhancesthegrowthofcc531ratcolorectalcancercellsbothinvitroandinvivo AT mikelgonzalezarribas partialhepatectomyenhancesthegrowthofcc531ratcolorectalcancercellsbothinvitroandinvivo AT inakidiazsanz partialhepatectomyenhancesthegrowthofcc531ratcolorectalcancercellsbothinvitroandinvivo AT teodoropalomares partialhepatectomyenhancesthegrowthofcc531ratcolorectalcancercellsbothinvitroandinvivo AT ignaciogarciaalonso partialhepatectomyenhancesthegrowthofcc531ratcolorectalcancercellsbothinvitroandinvivo
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Partial hepatectomy enhances the growth of CC531 rat colorectal cancer cells both in vitro and in vivo

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