Serous Membrane Detachment with Ultrasonic Homogenizer Improves Engraftment of Fetal Liver to Liver Surface in a Rat Model of Cirrhosis

Liver transplantation is the most effective treatment for end-stage cirrhosis. However, due to serious donor shortages, new treatments to replace liver transplantation are sorely needed. Recent studies have focused on novel therapeutic methods using hepatocytes and induced pluripotent stem cells, we...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yumi Kawakatsu-Hatada, Soichiro Murata, Akihiro Mori, Kodai Kimura, Hideki Taniguchi
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	liver transplantation liver cirrhosis serous membrane Cavitron Ultrasonic Surgical Aspirator Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/ccffbd2dd18241b7af409b3ca636e063
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ccffbd2dd18241b7af409b3ca636e063
record_format	dspace
spelling	oai:doaj.org-article:ccffbd2dd18241b7af409b3ca636e0632021-11-11T17:04:16ZSerous Membrane Detachment with Ultrasonic Homogenizer Improves Engraftment of Fetal Liver to Liver Surface in a Rat Model of Cirrhosis10.3390/ijms2221115891422-00671661-6596https://doaj.org/article/ccffbd2dd18241b7af409b3ca636e0632021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11589https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Liver transplantation is the most effective treatment for end-stage cirrhosis. However, due to serious donor shortages, new treatments to replace liver transplantation are sorely needed. Recent studies have focused on novel therapeutic methods using hepatocytes and induced pluripotent stem cells, we try hard to develop methods for transplanting these cells to the liver surface. In the present study, we evaluated several methods for their efficiency in the detachment of serous membrane covering the liver surface for transplantation to the liver surface. The liver surface of dipeptidyl peptidase IV (DPPIV)-deficient rats in a cirrhosis model was detached by various methods, and then fetal livers from DPPIV-positive rats were transplanted. We found that the engraftment rate and area as well as the liver function were improved in rats undergoing transplantation following serous membrane detachment with an ultrasonic homogenizer, which mimics the Cavitron Ultrasonic Surgical Aspirator<sup>®</sup> (CUSA), compared with no detachment. Furthermore, the bleeding amount was lower with the ultrasonic homogenizer method than with the needle and electric scalpel methods. These findings provide evidence that transplantation to the liver surface with serous membrane detachment using CUSA might contribute to the development of new treatments for cirrhosis using cells or tissues.Yumi Kawakatsu-HatadaSoichiro MurataAkihiro MoriKodai KimuraHideki TaniguchiMDPI AGarticleliver transplantationliver cirrhosisserous membraneCavitron Ultrasonic Surgical AspiratorBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11589, p 11589 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	liver transplantation liver cirrhosis serous membrane Cavitron Ultrasonic Surgical Aspirator Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	liver transplantation liver cirrhosis serous membrane Cavitron Ultrasonic Surgical Aspirator Biology (General) QH301-705.5 Chemistry QD1-999 Yumi Kawakatsu-Hatada Soichiro Murata Akihiro Mori Kodai Kimura Hideki Taniguchi Serous Membrane Detachment with Ultrasonic Homogenizer Improves Engraftment of Fetal Liver to Liver Surface in a Rat Model of Cirrhosis
description	Liver transplantation is the most effective treatment for end-stage cirrhosis. However, due to serious donor shortages, new treatments to replace liver transplantation are sorely needed. Recent studies have focused on novel therapeutic methods using hepatocytes and induced pluripotent stem cells, we try hard to develop methods for transplanting these cells to the liver surface. In the present study, we evaluated several methods for their efficiency in the detachment of serous membrane covering the liver surface for transplantation to the liver surface. The liver surface of dipeptidyl peptidase IV (DPPIV)-deficient rats in a cirrhosis model was detached by various methods, and then fetal livers from DPPIV-positive rats were transplanted. We found that the engraftment rate and area as well as the liver function were improved in rats undergoing transplantation following serous membrane detachment with an ultrasonic homogenizer, which mimics the Cavitron Ultrasonic Surgical Aspirator<sup>®</sup> (CUSA), compared with no detachment. Furthermore, the bleeding amount was lower with the ultrasonic homogenizer method than with the needle and electric scalpel methods. These findings provide evidence that transplantation to the liver surface with serous membrane detachment using CUSA might contribute to the development of new treatments for cirrhosis using cells or tissues.
format	article
author	Yumi Kawakatsu-Hatada Soichiro Murata Akihiro Mori Kodai Kimura Hideki Taniguchi
author_facet	Yumi Kawakatsu-Hatada Soichiro Murata Akihiro Mori Kodai Kimura Hideki Taniguchi
author_sort	Yumi Kawakatsu-Hatada
title	Serous Membrane Detachment with Ultrasonic Homogenizer Improves Engraftment of Fetal Liver to Liver Surface in a Rat Model of Cirrhosis
title_short	Serous Membrane Detachment with Ultrasonic Homogenizer Improves Engraftment of Fetal Liver to Liver Surface in a Rat Model of Cirrhosis
title_full	Serous Membrane Detachment with Ultrasonic Homogenizer Improves Engraftment of Fetal Liver to Liver Surface in a Rat Model of Cirrhosis
title_fullStr	Serous Membrane Detachment with Ultrasonic Homogenizer Improves Engraftment of Fetal Liver to Liver Surface in a Rat Model of Cirrhosis
title_full_unstemmed	Serous Membrane Detachment with Ultrasonic Homogenizer Improves Engraftment of Fetal Liver to Liver Surface in a Rat Model of Cirrhosis
title_sort	serous membrane detachment with ultrasonic homogenizer improves engraftment of fetal liver to liver surface in a rat model of cirrhosis
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/ccffbd2dd18241b7af409b3ca636e063
work_keys_str_mv	AT yumikawakatsuhatada serousmembranedetachmentwithultrasonichomogenizerimprovesengraftmentoffetallivertoliversurfaceinaratmodelofcirrhosis AT soichiromurata serousmembranedetachmentwithultrasonichomogenizerimprovesengraftmentoffetallivertoliversurfaceinaratmodelofcirrhosis AT akihiromori serousmembranedetachmentwithultrasonichomogenizerimprovesengraftmentoffetallivertoliversurfaceinaratmodelofcirrhosis AT kodaikimura serousmembranedetachmentwithultrasonichomogenizerimprovesengraftmentoffetallivertoliversurfaceinaratmodelofcirrhosis AT hidekitaniguchi serousmembranedetachmentwithultrasonichomogenizerimprovesengraftmentoffetallivertoliversurfaceinaratmodelofcirrhosis
_version_	1718432204494733312

Serous Membrane Detachment with Ultrasonic Homogenizer Improves Engraftment of Fetal Liver to Liver Surface in a Rat Model of Cirrhosis

Ejemplares similares