Addressing the challenges of E-cigarette safety profiling by assessment of pulmonary toxicological response in bronchial and alveolar mucosa models
Abstract Limited toxicity data on electronic cigarette (ECIG) impede evidence-based policy recommendations. We compared two popular mixed fruit flavored ECIG-liquids with and without nicotine aerosolized at 40 W (E-smoke) with respect to particle number concentrations, chemical composition, and resp...
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Nature Portfolio
2020
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oai:doaj.org-article:cd03240c31f6416eae94feae916263532021-12-02T16:08:46ZAddressing the challenges of E-cigarette safety profiling by assessment of pulmonary toxicological response in bronchial and alveolar mucosa models10.1038/s41598-020-77452-w2045-2322https://doaj.org/article/cd03240c31f6416eae94feae916263532020-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-77452-whttps://doaj.org/toc/2045-2322Abstract Limited toxicity data on electronic cigarette (ECIG) impede evidence-based policy recommendations. We compared two popular mixed fruit flavored ECIG-liquids with and without nicotine aerosolized at 40 W (E-smoke) with respect to particle number concentrations, chemical composition, and response on physiologically relevant human bronchial and alveolar lung mucosa models cultured at air–liquid interface. E-smoke was characterized by significantly increased particle number concentrations with increased wattage (25, 40, and 55 W) and nicotine presence. The chemical composition of E-smoke differed across the two tested flavors in terms of cytotoxic compounds including p-benzoquinone, nicotyrine, and flavoring agents (for example vanillin, ethyl vanillin). Significant differences in the expression of markers for pro-inflammation, oxidative stress, tissue injury/repair, alarm anti-protease, anti-microbial defense, epithelial barrier function, and epigenetic modification were observed between the flavors, nicotine content, and/ or lung models (bronchial or alveolar). Our findings indicate that ECIG toxicity is influenced by combination of multiple factors including flavor, nicotine content, vaping regime, and the region of respiratory tree (bronchial or alveolar). Toxic chemicals and flavoring agents detected in high concentrations in the E-smoke of each flavor warrant independent evaluation for their specific role in imparting toxicity. Therefore, multi-disciplinary approaches are warranted for comprehensive safety profiling of ECIG.Koustav GangulyAxel NordströmTania A. ThimrajMizanur RahmanMalin RamströmShanzina I. SompaElizabeth Z. LinFiona O’BrienJeremy KoelmelLena ErnstgårdGunnar JohansonKrystal J. Godri PollittLena PalmbergSwapna UpadhyayNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-15 (2020) |
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DOAJ |
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DOAJ |
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EN |
| topic |
Medicine R Science Q |
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Medicine R Science Q Koustav Ganguly Axel Nordström Tania A. Thimraj Mizanur Rahman Malin Ramström Shanzina I. Sompa Elizabeth Z. Lin Fiona O’Brien Jeremy Koelmel Lena Ernstgård Gunnar Johanson Krystal J. Godri Pollitt Lena Palmberg Swapna Upadhyay Addressing the challenges of E-cigarette safety profiling by assessment of pulmonary toxicological response in bronchial and alveolar mucosa models |
| description |
Abstract Limited toxicity data on electronic cigarette (ECIG) impede evidence-based policy recommendations. We compared two popular mixed fruit flavored ECIG-liquids with and without nicotine aerosolized at 40 W (E-smoke) with respect to particle number concentrations, chemical composition, and response on physiologically relevant human bronchial and alveolar lung mucosa models cultured at air–liquid interface. E-smoke was characterized by significantly increased particle number concentrations with increased wattage (25, 40, and 55 W) and nicotine presence. The chemical composition of E-smoke differed across the two tested flavors in terms of cytotoxic compounds including p-benzoquinone, nicotyrine, and flavoring agents (for example vanillin, ethyl vanillin). Significant differences in the expression of markers for pro-inflammation, oxidative stress, tissue injury/repair, alarm anti-protease, anti-microbial defense, epithelial barrier function, and epigenetic modification were observed between the flavors, nicotine content, and/ or lung models (bronchial or alveolar). Our findings indicate that ECIG toxicity is influenced by combination of multiple factors including flavor, nicotine content, vaping regime, and the region of respiratory tree (bronchial or alveolar). Toxic chemicals and flavoring agents detected in high concentrations in the E-smoke of each flavor warrant independent evaluation for their specific role in imparting toxicity. Therefore, multi-disciplinary approaches are warranted for comprehensive safety profiling of ECIG. |
| format |
article |
| author |
Koustav Ganguly Axel Nordström Tania A. Thimraj Mizanur Rahman Malin Ramström Shanzina I. Sompa Elizabeth Z. Lin Fiona O’Brien Jeremy Koelmel Lena Ernstgård Gunnar Johanson Krystal J. Godri Pollitt Lena Palmberg Swapna Upadhyay |
| author_facet |
Koustav Ganguly Axel Nordström Tania A. Thimraj Mizanur Rahman Malin Ramström Shanzina I. Sompa Elizabeth Z. Lin Fiona O’Brien Jeremy Koelmel Lena Ernstgård Gunnar Johanson Krystal J. Godri Pollitt Lena Palmberg Swapna Upadhyay |
| author_sort |
Koustav Ganguly |
| title |
Addressing the challenges of E-cigarette safety profiling by assessment of pulmonary toxicological response in bronchial and alveolar mucosa models |
| title_short |
Addressing the challenges of E-cigarette safety profiling by assessment of pulmonary toxicological response in bronchial and alveolar mucosa models |
| title_full |
Addressing the challenges of E-cigarette safety profiling by assessment of pulmonary toxicological response in bronchial and alveolar mucosa models |
| title_fullStr |
Addressing the challenges of E-cigarette safety profiling by assessment of pulmonary toxicological response in bronchial and alveolar mucosa models |
| title_full_unstemmed |
Addressing the challenges of E-cigarette safety profiling by assessment of pulmonary toxicological response in bronchial and alveolar mucosa models |
| title_sort |
addressing the challenges of e-cigarette safety profiling by assessment of pulmonary toxicological response in bronchial and alveolar mucosa models |
| publisher |
Nature Portfolio |
| publishDate |
2020 |
| url |
https://doaj.org/article/cd03240c31f6416eae94feae91626353 |
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