Enhanced growth of endothelial precursor cells on PCG-matrix facilitates accelerated, fibrosis-free, wound healing: a diabetic mouse model.

Enhanced growth of endothelial precursor cells on PCG-matrix facilitates accelerated, fibrosis-free, wound healing: a diabetic mouse model.

Diabetes mellitus (DM)-induced endothelial progenitor cell (EPC) dysfunction causes impaired wound healing, which can be rescued by delivery of large numbers of 'normal' EPCs onto such wounds. The principal challenges herein are (a) the high number of EPCs required and (b) their sustained...

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Autores principales: Meghana Kanitkar, Amit Jaiswal, Rucha Deshpande, Jayesh Bellare, Vaijayanti P Kale
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Publicado: Public Library of Science (PLoS) 2013
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spelling oai:doaj.org-article:cd03f767459f460e9de6616a5e2a237c2021-11-18T09:02:38ZEnhanced growth of endothelial precursor cells on PCG-matrix facilitates accelerated, fibrosis-free, wound healing: a diabetic mouse model.1932-620310.1371/journal.pone.0069960https://doaj.org/article/cd03f767459f460e9de6616a5e2a237c2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23922871/?tool=EBIhttps://doaj.org/toc/1932-6203Diabetes mellitus (DM)-induced endothelial progenitor cell (EPC) dysfunction causes impaired wound healing, which can be rescued by delivery of large numbers of 'normal' EPCs onto such wounds. The principal challenges herein are (a) the high number of EPCs required and (b) their sustained delivery onto the wounds. Most of the currently available scaffolds either serve as passive devices for cellular delivery or allow adherence and proliferation, but not both. This clearly indicates that matrices possessing both attributes are 'the need of the day' for efficient healing of diabetic wounds. Therefore, we developed a system that not only allows selective enrichment and expansion of EPCs, but also efficiently delivers them onto the wounds. Murine bone marrow-derived mononuclear cells (MNCs) were seeded onto a PolyCaprolactone-Gelatin (PCG) nano-fiber matrix that offers a combined advantage of strength, biocompatibility wettability; and cultured them in EGM2 to allow EPC growth. The efficacy of the PCG matrix in supporting the EPC growth and delivery was assessed by various in vitro parameters. Its efficacy in diabetic wound healing was assessed by a topical application of the PCG-EPCs onto diabetic wounds. The PCG matrix promoted a high-level attachment of EPCs and enhanced their growth, colony formation, and proliferation without compromising their viability as compared to Poly L-lactic acid (PLLA) and Vitronectin (VN), the matrix and non-matrix controls respectively. The PCG-matrix also allowed a sustained chemotactic migration of EPCs in vitro. The matrix-effected sustained delivery of EPCs onto the diabetic wounds resulted in an enhanced fibrosis-free wound healing as compared to the controls. Our data, thus, highlight the novel therapeutic potential of PCG-EPCs as a combined 'growth and delivery system' to achieve an accelerated fibrosis-free healing of dermal lesions, including diabetic wounds.Meghana KanitkarAmit JaiswalRucha DeshpandeJayesh BellareVaijayanti P KalePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 7, p e69960 (2013)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Meghana Kanitkar
Amit Jaiswal
Rucha Deshpande
Jayesh Bellare
Vaijayanti P Kale
Enhanced growth of endothelial precursor cells on PCG-matrix facilitates accelerated, fibrosis-free, wound healing: a diabetic mouse model.
description Diabetes mellitus (DM)-induced endothelial progenitor cell (EPC) dysfunction causes impaired wound healing, which can be rescued by delivery of large numbers of 'normal' EPCs onto such wounds. The principal challenges herein are (a) the high number of EPCs required and (b) their sustained delivery onto the wounds. Most of the currently available scaffolds either serve as passive devices for cellular delivery or allow adherence and proliferation, but not both. This clearly indicates that matrices possessing both attributes are 'the need of the day' for efficient healing of diabetic wounds. Therefore, we developed a system that not only allows selective enrichment and expansion of EPCs, but also efficiently delivers them onto the wounds. Murine bone marrow-derived mononuclear cells (MNCs) were seeded onto a PolyCaprolactone-Gelatin (PCG) nano-fiber matrix that offers a combined advantage of strength, biocompatibility wettability; and cultured them in EGM2 to allow EPC growth. The efficacy of the PCG matrix in supporting the EPC growth and delivery was assessed by various in vitro parameters. Its efficacy in diabetic wound healing was assessed by a topical application of the PCG-EPCs onto diabetic wounds. The PCG matrix promoted a high-level attachment of EPCs and enhanced their growth, colony formation, and proliferation without compromising their viability as compared to Poly L-lactic acid (PLLA) and Vitronectin (VN), the matrix and non-matrix controls respectively. The PCG-matrix also allowed a sustained chemotactic migration of EPCs in vitro. The matrix-effected sustained delivery of EPCs onto the diabetic wounds resulted in an enhanced fibrosis-free wound healing as compared to the controls. Our data, thus, highlight the novel therapeutic potential of PCG-EPCs as a combined 'growth and delivery system' to achieve an accelerated fibrosis-free healing of dermal lesions, including diabetic wounds.
format article
author Meghana Kanitkar
Amit Jaiswal
Rucha Deshpande
Jayesh Bellare
Vaijayanti P Kale
author_facet Meghana Kanitkar
Amit Jaiswal
Rucha Deshpande
Jayesh Bellare
Vaijayanti P Kale
author_sort Meghana Kanitkar
title Enhanced growth of endothelial precursor cells on PCG-matrix facilitates accelerated, fibrosis-free, wound healing: a diabetic mouse model.
title_short Enhanced growth of endothelial precursor cells on PCG-matrix facilitates accelerated, fibrosis-free, wound healing: a diabetic mouse model.
title_full Enhanced growth of endothelial precursor cells on PCG-matrix facilitates accelerated, fibrosis-free, wound healing: a diabetic mouse model.
title_fullStr Enhanced growth of endothelial precursor cells on PCG-matrix facilitates accelerated, fibrosis-free, wound healing: a diabetic mouse model.
title_full_unstemmed Enhanced growth of endothelial precursor cells on PCG-matrix facilitates accelerated, fibrosis-free, wound healing: a diabetic mouse model.
title_sort enhanced growth of endothelial precursor cells on pcg-matrix facilitates accelerated, fibrosis-free, wound healing: a diabetic mouse model.
publisher Public Library of Science (PLoS)
publishDate 2013
url https://doaj.org/article/cd03f767459f460e9de6616a5e2a237c
work_keys_str_mv AT meghanakanitkar enhancedgrowthofendothelialprecursorcellsonpcgmatrixfacilitatesacceleratedfibrosisfreewoundhealingadiabeticmousemodel
AT amitjaiswal enhancedgrowthofendothelialprecursorcellsonpcgmatrixfacilitatesacceleratedfibrosisfreewoundhealingadiabeticmousemodel
AT ruchadeshpande enhancedgrowthofendothelialprecursorcellsonpcgmatrixfacilitatesacceleratedfibrosisfreewoundhealingadiabeticmousemodel
AT jayeshbellare enhancedgrowthofendothelialprecursorcellsonpcgmatrixfacilitatesacceleratedfibrosisfreewoundhealingadiabeticmousemodel
AT vaijayantipkale enhancedgrowthofendothelialprecursorcellsonpcgmatrixfacilitatesacceleratedfibrosisfreewoundhealingadiabeticmousemodel
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