Three-dimensional virtual histology in unprocessed resected tissues with photoacoustic remote sensing (PARS) microscopy and optical coherence tomography (OCT)

Abstract Histological images are critical in the diagnosis and treatment of cancers. Unfortunately, current methods for capturing these microscopy images require resource intensive tissue preparation that may delay diagnosis for days or weeks. To streamline this process, clinicians are limited to as...

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Autores principales:	Benjamin R. Ecclestone, Zohreh Hosseinaee, Nima Abbasi, Kevan Bell, Deepak Dinakaran, John R. Mackey, Parsin Haji Reza
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cd10d4fca1594117be0828838cbfb92a
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spelling	oai:doaj.org-article:cd10d4fca1594117be0828838cbfb92a2021-12-02T16:31:50ZThree-dimensional virtual histology in unprocessed resected tissues with photoacoustic remote sensing (PARS) microscopy and optical coherence tomography (OCT)10.1038/s41598-021-93222-82045-2322https://doaj.org/article/cd10d4fca1594117be0828838cbfb92a2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93222-8https://doaj.org/toc/2045-2322Abstract Histological images are critical in the diagnosis and treatment of cancers. Unfortunately, current methods for capturing these microscopy images require resource intensive tissue preparation that may delay diagnosis for days or weeks. To streamline this process, clinicians are limited to assessing small macroscopically representative subsets of tissues. Here, a combined photoacoustic remote sensing (PARS) microscope and swept source optical coherence tomography system designed to circumvent these diagnostic limitations is presented. The proposed multimodal microscope provides label-free three-dimensional depth resolved virtual histology visualizations, capturing nuclear and extranuclear tissue morphology directly on thick unprocessed specimens. The capabilities of the proposed method are demonstrated directly in unprocessed formalin fixed resected tissues. The first images of nuclear contrast in resected human tissues, and the first three-dimensional visualization of subsurface nuclear morphology in resected Rattus tissues, captured with a non-contact photoacoustic system are presented here. Moreover, the proposed system captures the first co-registered OCT and PARS images enabling direct histological assessment of unprocessed tissues. This work represents a vital step towards the development of a rapid histological imaging modality to circumvent the limitations of current histopathology techniques.Benjamin R. EcclestoneZohreh HosseinaeeNima AbbasiKevan BellDeepak DinakaranJohn R. MackeyParsin Haji RezaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Benjamin R. Ecclestone Zohreh Hosseinaee Nima Abbasi Kevan Bell Deepak Dinakaran John R. Mackey Parsin Haji Reza Three-dimensional virtual histology in unprocessed resected tissues with photoacoustic remote sensing (PARS) microscopy and optical coherence tomography (OCT)
description	Abstract Histological images are critical in the diagnosis and treatment of cancers. Unfortunately, current methods for capturing these microscopy images require resource intensive tissue preparation that may delay diagnosis for days or weeks. To streamline this process, clinicians are limited to assessing small macroscopically representative subsets of tissues. Here, a combined photoacoustic remote sensing (PARS) microscope and swept source optical coherence tomography system designed to circumvent these diagnostic limitations is presented. The proposed multimodal microscope provides label-free three-dimensional depth resolved virtual histology visualizations, capturing nuclear and extranuclear tissue morphology directly on thick unprocessed specimens. The capabilities of the proposed method are demonstrated directly in unprocessed formalin fixed resected tissues. The first images of nuclear contrast in resected human tissues, and the first three-dimensional visualization of subsurface nuclear morphology in resected Rattus tissues, captured with a non-contact photoacoustic system are presented here. Moreover, the proposed system captures the first co-registered OCT and PARS images enabling direct histological assessment of unprocessed tissues. This work represents a vital step towards the development of a rapid histological imaging modality to circumvent the limitations of current histopathology techniques.
format	article
author	Benjamin R. Ecclestone Zohreh Hosseinaee Nima Abbasi Kevan Bell Deepak Dinakaran John R. Mackey Parsin Haji Reza
author_facet	Benjamin R. Ecclestone Zohreh Hosseinaee Nima Abbasi Kevan Bell Deepak Dinakaran John R. Mackey Parsin Haji Reza
author_sort	Benjamin R. Ecclestone
title	Three-dimensional virtual histology in unprocessed resected tissues with photoacoustic remote sensing (PARS) microscopy and optical coherence tomography (OCT)
title_short	Three-dimensional virtual histology in unprocessed resected tissues with photoacoustic remote sensing (PARS) microscopy and optical coherence tomography (OCT)
title_full	Three-dimensional virtual histology in unprocessed resected tissues with photoacoustic remote sensing (PARS) microscopy and optical coherence tomography (OCT)
title_fullStr	Three-dimensional virtual histology in unprocessed resected tissues with photoacoustic remote sensing (PARS) microscopy and optical coherence tomography (OCT)
title_full_unstemmed	Three-dimensional virtual histology in unprocessed resected tissues with photoacoustic remote sensing (PARS) microscopy and optical coherence tomography (OCT)
title_sort	three-dimensional virtual histology in unprocessed resected tissues with photoacoustic remote sensing (pars) microscopy and optical coherence tomography (oct)
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/cd10d4fca1594117be0828838cbfb92a
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Three-dimensional virtual histology in unprocessed resected tissues with photoacoustic remote sensing (PARS) microscopy and optical coherence tomography (OCT)

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