Isolation and characterization of a lytic bacteriophage against Pseudomonas aeruginosa

Abstract In recent years, the use of bacteriophages (or 'phages') against multidrug-resistant (MDR) bacteria including Pseudomonas aeruginosa has drawn considerable attention, globally. In this work, we report the isolation and detailed characterization of a highly lytic Pseudomonasphage D...

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Autores principales:	Sonika Sharma, Sibnarayan Datta, Soumya Chatterjee, Moumita Dutta, Jhuma Samanta, Mohan G. Vairale, Rajeev Gupta, Vijay Veer, Sanjai K. Dwivedi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cd12c250461c4d71b16dadc0bc7f84d5
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spelling	oai:doaj.org-article:cd12c250461c4d71b16dadc0bc7f84d52021-12-02T19:16:46ZIsolation and characterization of a lytic bacteriophage against Pseudomonas aeruginosa10.1038/s41598-021-98457-z2045-2322https://doaj.org/article/cd12c250461c4d71b16dadc0bc7f84d52021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98457-zhttps://doaj.org/toc/2045-2322Abstract In recent years, the use of bacteriophages (or 'phages') against multidrug-resistant (MDR) bacteria including Pseudomonas aeruginosa has drawn considerable attention, globally. In this work, we report the isolation and detailed characterization of a highly lytic Pseudomonasphage DRL-P1 isolated from wastewater. Under TEM, DRL-P1 appeared as a member of the phage family Myoviridae. DRL-P1 featured rapid adsorption (~ 5 min), short-latency (~ 30 min), and large burst size (~ 100 PFU per infected cell). DRL-P1 can withstand a wide temperature range (4 °C to 40 °C) and pH (5.0 to 10.0) conditions. The 66,243 bp DRL-P1 genome (MN564818) encodes at least 93 ORFs, of which 36 were functionally annotated based on homology with similar phage proteins available in the databases. Comparative analyses of related genomes suggest an independent evolutionary history and discrete taxonomic position of DRL-P1 within genus Pbunavirus. No toxin or antibiotic resistance genes was identified. DRL-P1 is tolerant to lyophilization and encapsulation techniques and retained lytic activity even after 18 months of storage. We also demonstrated decontaminating potentials of DRL-P1 in vitro, on an artificially contaminated cover-slip model. To the best of our knowledge, this is the first Pbunavirus to be reported from India. Our study suggests DRL-P1 as a potential candidate for various applications.Sonika SharmaSibnarayan DattaSoumya ChatterjeeMoumita DuttaJhuma SamantaMohan G. VairaleRajeev GuptaVijay VeerSanjai K. DwivediNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-22 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sonika Sharma Sibnarayan Datta Soumya Chatterjee Moumita Dutta Jhuma Samanta Mohan G. Vairale Rajeev Gupta Vijay Veer Sanjai K. Dwivedi Isolation and characterization of a lytic bacteriophage against Pseudomonas aeruginosa
description	Abstract In recent years, the use of bacteriophages (or 'phages') against multidrug-resistant (MDR) bacteria including Pseudomonas aeruginosa has drawn considerable attention, globally. In this work, we report the isolation and detailed characterization of a highly lytic Pseudomonasphage DRL-P1 isolated from wastewater. Under TEM, DRL-P1 appeared as a member of the phage family Myoviridae. DRL-P1 featured rapid adsorption (~ 5 min), short-latency (~ 30 min), and large burst size (~ 100 PFU per infected cell). DRL-P1 can withstand a wide temperature range (4 °C to 40 °C) and pH (5.0 to 10.0) conditions. The 66,243 bp DRL-P1 genome (MN564818) encodes at least 93 ORFs, of which 36 were functionally annotated based on homology with similar phage proteins available in the databases. Comparative analyses of related genomes suggest an independent evolutionary history and discrete taxonomic position of DRL-P1 within genus Pbunavirus. No toxin or antibiotic resistance genes was identified. DRL-P1 is tolerant to lyophilization and encapsulation techniques and retained lytic activity even after 18 months of storage. We also demonstrated decontaminating potentials of DRL-P1 in vitro, on an artificially contaminated cover-slip model. To the best of our knowledge, this is the first Pbunavirus to be reported from India. Our study suggests DRL-P1 as a potential candidate for various applications.
format	article
author	Sonika Sharma Sibnarayan Datta Soumya Chatterjee Moumita Dutta Jhuma Samanta Mohan G. Vairale Rajeev Gupta Vijay Veer Sanjai K. Dwivedi
author_facet	Sonika Sharma Sibnarayan Datta Soumya Chatterjee Moumita Dutta Jhuma Samanta Mohan G. Vairale Rajeev Gupta Vijay Veer Sanjai K. Dwivedi
author_sort	Sonika Sharma
title	Isolation and characterization of a lytic bacteriophage against Pseudomonas aeruginosa
title_short	Isolation and characterization of a lytic bacteriophage against Pseudomonas aeruginosa
title_full	Isolation and characterization of a lytic bacteriophage against Pseudomonas aeruginosa
title_fullStr	Isolation and characterization of a lytic bacteriophage against Pseudomonas aeruginosa
title_full_unstemmed	Isolation and characterization of a lytic bacteriophage against Pseudomonas aeruginosa
title_sort	isolation and characterization of a lytic bacteriophage against pseudomonas aeruginosa
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/cd12c250461c4d71b16dadc0bc7f84d5
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Isolation and characterization of a lytic bacteriophage against Pseudomonas aeruginosa

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