Divalent cations can control a switch-like behavior in heterotypic and homotypic RNA coacervates

Abstract Liquid-liquid phase separation (LLPS) of RNA-protein complexes plays a major role in the cellular function of membraneless organelles (MLOs). MLOs are sensitive to changes in cellular conditions, such as fluctuations in cytoplasmic ion concentrations. To investigate the effect of these chan...

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Autores principales:	Paulo L. Onuchic, Anthony N. Milin, Ibraheem Alshareedah, Ashok A. Deniz, Priya R. Banerjee
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cd138c16e3a643ada045678203d4f324
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spelling	oai:doaj.org-article:cd138c16e3a643ada045678203d4f3242021-12-02T15:09:33ZDivalent cations can control a switch-like behavior in heterotypic and homotypic RNA coacervates10.1038/s41598-019-48457-x2045-2322https://doaj.org/article/cd138c16e3a643ada045678203d4f3242019-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-48457-xhttps://doaj.org/toc/2045-2322Abstract Liquid-liquid phase separation (LLPS) of RNA-protein complexes plays a major role in the cellular function of membraneless organelles (MLOs). MLOs are sensitive to changes in cellular conditions, such as fluctuations in cytoplasmic ion concentrations. To investigate the effect of these changes on MLOs, we studied the influence of divalent cations on the physical and chemical properties of RNA coacervates. Using a model system comprised of an arginine-rich peptide and RNA, we predicted and observed that variations in signaling cations exert interaction-dependent effects on RNA LLPS. Changing the ionic environment has opposing effects on the propensity for heterotypic peptide-RNA and homotypic RNA LLPS, which results in a switch between coacervate types. Furthermore, divalent ion variations continuously tune the microenvironments and fluid properties of heterotypic and homotypic droplets. Our results may provide a general mechanism for modulating the biochemical environment of RNA coacervates in a cellular context.Paulo L. OnuchicAnthony N. MilinIbraheem AlshareedahAshok A. DenizPriya R. BanerjeeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-10 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Paulo L. Onuchic Anthony N. Milin Ibraheem Alshareedah Ashok A. Deniz Priya R. Banerjee Divalent cations can control a switch-like behavior in heterotypic and homotypic RNA coacervates
description	Abstract Liquid-liquid phase separation (LLPS) of RNA-protein complexes plays a major role in the cellular function of membraneless organelles (MLOs). MLOs are sensitive to changes in cellular conditions, such as fluctuations in cytoplasmic ion concentrations. To investigate the effect of these changes on MLOs, we studied the influence of divalent cations on the physical and chemical properties of RNA coacervates. Using a model system comprised of an arginine-rich peptide and RNA, we predicted and observed that variations in signaling cations exert interaction-dependent effects on RNA LLPS. Changing the ionic environment has opposing effects on the propensity for heterotypic peptide-RNA and homotypic RNA LLPS, which results in a switch between coacervate types. Furthermore, divalent ion variations continuously tune the microenvironments and fluid properties of heterotypic and homotypic droplets. Our results may provide a general mechanism for modulating the biochemical environment of RNA coacervates in a cellular context.
format	article
author	Paulo L. Onuchic Anthony N. Milin Ibraheem Alshareedah Ashok A. Deniz Priya R. Banerjee
author_facet	Paulo L. Onuchic Anthony N. Milin Ibraheem Alshareedah Ashok A. Deniz Priya R. Banerjee
author_sort	Paulo L. Onuchic
title	Divalent cations can control a switch-like behavior in heterotypic and homotypic RNA coacervates
title_short	Divalent cations can control a switch-like behavior in heterotypic and homotypic RNA coacervates
title_full	Divalent cations can control a switch-like behavior in heterotypic and homotypic RNA coacervates
title_fullStr	Divalent cations can control a switch-like behavior in heterotypic and homotypic RNA coacervates
title_full_unstemmed	Divalent cations can control a switch-like behavior in heterotypic and homotypic RNA coacervates
title_sort	divalent cations can control a switch-like behavior in heterotypic and homotypic rna coacervates
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/cd138c16e3a643ada045678203d4f324
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Divalent cations can control a switch-like behavior in heterotypic and homotypic RNA coacervates

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