The C-terminal region affects the activity of photoactivated adenylyl cyclase from Oscillatoria acuminata

Abstract Photoactivated adenylyl cyclase (PAC) is a unique protein that, upon blue light exposure, catalyzes cAMP production. The crystal structures of two PACs, from Oscillatoria acuminata (OaPAC) and Beggiatoa sp. (bPAC), have been solved, and they show a high degree of similarity. However, the ph...

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Autores principales:	Minako Hirano, Masumi Takebe, Tomoya Ishido, Toru Ide, Shigeru Matsunaga
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cd14bbaf12904f38a1652d577a1482c6
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spelling	oai:doaj.org-article:cd14bbaf12904f38a1652d577a1482c62021-12-02T15:12:22ZThe C-terminal region affects the activity of photoactivated adenylyl cyclase from Oscillatoria acuminata10.1038/s41598-019-56721-32045-2322https://doaj.org/article/cd14bbaf12904f38a1652d577a1482c62019-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-56721-3https://doaj.org/toc/2045-2322Abstract Photoactivated adenylyl cyclase (PAC) is a unique protein that, upon blue light exposure, catalyzes cAMP production. The crystal structures of two PACs, from Oscillatoria acuminata (OaPAC) and Beggiatoa sp. (bPAC), have been solved, and they show a high degree of similarity. However, the photoactivity of OaPAC is much lower than that of bPAC, and the regulatory mechanism of PAC photoactivity, which induces the difference in activity between OaPAC and bPAC, has not yet been clarified. Here, we investigated the role of the C-terminal region in OaPAC, the length of which is the only notable difference from bPAC. We found that the photoactivity of OaPAC was inversely proportional to the C-terminal length. However, the deletion of more than nine amino acids did not further increase the activity, indicating that the nine amino acids at the C-terminal critically affect the photoactivity. Besides, absorption spectral features of light-sensing domains (BLUF domains) of the C-terminal deletion mutants showed similar light-dependent spectral shifts as in WT, indicating that the C-terminal region influences the activity without interacting with the BLUF domain. The study characterizes new PAC mutants with modified photoactivities, which could be useful as optogenetics tools.Minako HiranoMasumi TakebeTomoya IshidoToru IdeShigeru MatsunagaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-8 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Minako Hirano Masumi Takebe Tomoya Ishido Toru Ide Shigeru Matsunaga The C-terminal region affects the activity of photoactivated adenylyl cyclase from Oscillatoria acuminata
description	Abstract Photoactivated adenylyl cyclase (PAC) is a unique protein that, upon blue light exposure, catalyzes cAMP production. The crystal structures of two PACs, from Oscillatoria acuminata (OaPAC) and Beggiatoa sp. (bPAC), have been solved, and they show a high degree of similarity. However, the photoactivity of OaPAC is much lower than that of bPAC, and the regulatory mechanism of PAC photoactivity, which induces the difference in activity between OaPAC and bPAC, has not yet been clarified. Here, we investigated the role of the C-terminal region in OaPAC, the length of which is the only notable difference from bPAC. We found that the photoactivity of OaPAC was inversely proportional to the C-terminal length. However, the deletion of more than nine amino acids did not further increase the activity, indicating that the nine amino acids at the C-terminal critically affect the photoactivity. Besides, absorption spectral features of light-sensing domains (BLUF domains) of the C-terminal deletion mutants showed similar light-dependent spectral shifts as in WT, indicating that the C-terminal region influences the activity without interacting with the BLUF domain. The study characterizes new PAC mutants with modified photoactivities, which could be useful as optogenetics tools.
format	article
author	Minako Hirano Masumi Takebe Tomoya Ishido Toru Ide Shigeru Matsunaga
author_facet	Minako Hirano Masumi Takebe Tomoya Ishido Toru Ide Shigeru Matsunaga
author_sort	Minako Hirano
title	The C-terminal region affects the activity of photoactivated adenylyl cyclase from Oscillatoria acuminata
title_short	The C-terminal region affects the activity of photoactivated adenylyl cyclase from Oscillatoria acuminata
title_full	The C-terminal region affects the activity of photoactivated adenylyl cyclase from Oscillatoria acuminata
title_fullStr	The C-terminal region affects the activity of photoactivated adenylyl cyclase from Oscillatoria acuminata
title_full_unstemmed	The C-terminal region affects the activity of photoactivated adenylyl cyclase from Oscillatoria acuminata
title_sort	c-terminal region affects the activity of photoactivated adenylyl cyclase from oscillatoria acuminata
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/cd14bbaf12904f38a1652d577a1482c6
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The C-terminal region affects the activity of photoactivated adenylyl cyclase from Oscillatoria acuminata

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