The role of nuclear organization in trans-splicing based expression of heat shock protein 90 in Giardia lamblia.

Hsp90 gene of G. lamblia has a split nature comprising two ORFs separated by 777 kb on chromosome 5. The ORFs of the split gene on chromosome 5 undergo transcription to generate independent pre-mRNAs that join by a unique trans-splicing reaction that remains partially understood. The canonical cis-a...

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Autores principales:	Vinithra Iyer, Sheetal Tushir, Shreekant Verma, Sudeshna Majumdar, Srimonta Gayen, Rakesh Mishra, Utpal Tatu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/cd162f0c3b4c43cc9073ff06028d3341
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id	oai:doaj.org-article:cd162f0c3b4c43cc9073ff06028d3341
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spelling	oai:doaj.org-article:cd162f0c3b4c43cc9073ff06028d33412021-12-02T20:24:02ZThe role of nuclear organization in trans-splicing based expression of heat shock protein 90 in Giardia lamblia.1935-27271935-273510.1371/journal.pntd.0009810https://doaj.org/article/cd162f0c3b4c43cc9073ff06028d33412021-09-01T00:00:00Zhttps://doi.org/10.1371/journal.pntd.0009810https://doaj.org/toc/1935-2727https://doaj.org/toc/1935-2735Hsp90 gene of G. lamblia has a split nature comprising two ORFs separated by 777 kb on chromosome 5. The ORFs of the split gene on chromosome 5 undergo transcription to generate independent pre-mRNAs that join by a unique trans-splicing reaction that remains partially understood. The canonical cis-acting nucleotide elements such as 5'SS-GU, 3'SS-AG, polypyrimidine tract and branch point adenine are present in the independent pre-mRNAs and therefore trans-splicing of Hsp90 must be assisted by spliceosomes in vivo. Using an approach of RNA-protein pull down, we show that an RNA helicase selectively interacts with HspN pre-mRNA. Our experiments involving high resolution chromosome conformation capture technology as well as DNA FISH show that the trans-spliced genes of Giardia are in three-dimensional spatial proximity in the nucleus. Altogether our study provides a glimpse into the in vivo mechanisms involving protein factors as well as chromatin structure to facilitate the unique inter-molecular post-transcriptional stitching of split genes in G. lamblia.Vinithra IyerSheetal TushirShreekant VermaSudeshna MajumdarSrimonta GayenRakesh MishraUtpal TatuPublic Library of Science (PLoS)articleArctic medicine. Tropical medicineRC955-962Public aspects of medicineRA1-1270ENPLoS Neglected Tropical Diseases, Vol 15, Iss 9, p e0009810 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
spellingShingle	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Vinithra Iyer Sheetal Tushir Shreekant Verma Sudeshna Majumdar Srimonta Gayen Rakesh Mishra Utpal Tatu The role of nuclear organization in trans-splicing based expression of heat shock protein 90 in Giardia lamblia.
description	Hsp90 gene of G. lamblia has a split nature comprising two ORFs separated by 777 kb on chromosome 5. The ORFs of the split gene on chromosome 5 undergo transcription to generate independent pre-mRNAs that join by a unique trans-splicing reaction that remains partially understood. The canonical cis-acting nucleotide elements such as 5'SS-GU, 3'SS-AG, polypyrimidine tract and branch point adenine are present in the independent pre-mRNAs and therefore trans-splicing of Hsp90 must be assisted by spliceosomes in vivo. Using an approach of RNA-protein pull down, we show that an RNA helicase selectively interacts with HspN pre-mRNA. Our experiments involving high resolution chromosome conformation capture technology as well as DNA FISH show that the trans-spliced genes of Giardia are in three-dimensional spatial proximity in the nucleus. Altogether our study provides a glimpse into the in vivo mechanisms involving protein factors as well as chromatin structure to facilitate the unique inter-molecular post-transcriptional stitching of split genes in G. lamblia.
format	article
author	Vinithra Iyer Sheetal Tushir Shreekant Verma Sudeshna Majumdar Srimonta Gayen Rakesh Mishra Utpal Tatu
author_facet	Vinithra Iyer Sheetal Tushir Shreekant Verma Sudeshna Majumdar Srimonta Gayen Rakesh Mishra Utpal Tatu
author_sort	Vinithra Iyer
title	The role of nuclear organization in trans-splicing based expression of heat shock protein 90 in Giardia lamblia.
title_short	The role of nuclear organization in trans-splicing based expression of heat shock protein 90 in Giardia lamblia.
title_full	The role of nuclear organization in trans-splicing based expression of heat shock protein 90 in Giardia lamblia.
title_fullStr	The role of nuclear organization in trans-splicing based expression of heat shock protein 90 in Giardia lamblia.
title_full_unstemmed	The role of nuclear organization in trans-splicing based expression of heat shock protein 90 in Giardia lamblia.
title_sort	role of nuclear organization in trans-splicing based expression of heat shock protein 90 in giardia lamblia.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/cd162f0c3b4c43cc9073ff06028d3341
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The role of nuclear organization in trans-splicing based expression of heat shock protein 90 in Giardia lamblia.

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