Effect of dexmedetomidine on hippocampal neuron development and BDNF-TrkB signal expression in neonatal rats

Jie Lv, Wei Ou, Xiao-Hua Zou, Yin Yao, Jin-Li Wu Department of Anesthesia, Guizhou Medical University Affiliated Hospital, Guiyang, People’s Republic of China Abstract: The study aimed to explore the effect of dexmedetomidine (DEX) on hippocampal neuron development process and on molecul...

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Autores principales:	Lv J, Ou W, Zou XH, Yao Y, Wu JL
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	dexmedetomidine hippocampal neuron development BDNF-TrkB Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
Acceso en línea:	https://doaj.org/article/cd1acb1ecb3444dfb8b7f841d0548f91
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spelling	oai:doaj.org-article:cd1acb1ecb3444dfb8b7f841d0548f912021-12-02T07:14:57ZEffect of dexmedetomidine on hippocampal neuron development and BDNF-TrkB signal expression in neonatal rats1178-2021https://doaj.org/article/cd1acb1ecb3444dfb8b7f841d0548f912016-12-01T00:00:00Zhttps://www.dovepress.com/effect-of-dexmedetomidine-on-hippocampal-neuron-development-and-bdnf-t-peer-reviewed-article-NDThttps://doaj.org/toc/1178-2021Jie Lv, Wei Ou, Xiao-Hua Zou, Yin Yao, Jin-Li Wu Department of Anesthesia, Guizhou Medical University Affiliated Hospital, Guiyang, People’s Republic of China Abstract: The study aimed to explore the effect of dexmedetomidine (DEX) on hippocampal neuron development process and on molecular expression of brain-derived neurotrophic factor (BDNF)-tyrosine receptor kinase B (TrkB) signaling pathway in neonatal rats. The hippocampal neuron cells were isolated from newborn neonatal rats and cultured in vitro. One control group and three treated groups with 1, 10, and 100 µmol/L DEX were used for the study. Cell activity and apoptosis were detected by the MTT and terminal deoxynucleotidyl transferase-mediated biotinylated uridine triphosphate (UTP) nick end labeling assays. The synaptophysin (SYN) and postsynaptic density 95 (PSD95) were detected by quantitative polymerase chain reaction. There was no difference in the viability of neuron cells among the different dose groups of DEX and the control group during days 2-10 (P>0.05). Compared to the control group, there was no significant difference (P>0.05) in the expressions of SYN and PSD95 in the groups treated with 1 and 10 µmol/L DEX, whereas significant difference in the expression was observed in the group treated with 100 µmol/L DEX (P<0.01). Compared with the control group, the expression of BDNF was significantly upregulated (P<0.05) in the group treated with 100 µmol/L DEX. There were no significant differences in TrkB expression among the four groups. The expression of p-N-methyl-D-aspartate receptor increased with an increase in the concentration of DEX; however, only the high dose revealed a significant upregulation compared with the control group. The neuroprotective effect of DEX may be achieved by upregulating the expression of BDNF and phosphorylation level of N-methyl-D-aspartate receptor. Keywords: dexmedetomidine, hippocampal neuron, development, BDNF-TrkBLv JOu WZou XHYao YWu JLDove Medical Pressarticledexmedetomidinehippocampal neurondevelopmentBDNF-TrkBNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571Neurology. Diseases of the nervous systemRC346-429ENNeuropsychiatric Disease and Treatment, Vol Volume 12, Pp 3153-3159 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	dexmedetomidine hippocampal neuron development BDNF-TrkB Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
spellingShingle	dexmedetomidine hippocampal neuron development BDNF-TrkB Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429 Lv J Ou W Zou XH Yao Y Wu JL Effect of dexmedetomidine on hippocampal neuron development and BDNF-TrkB signal expression in neonatal rats
description	Jie Lv, Wei Ou, Xiao-Hua Zou, Yin Yao, Jin-Li Wu Department of Anesthesia, Guizhou Medical University Affiliated Hospital, Guiyang, People’s Republic of China Abstract: The study aimed to explore the effect of dexmedetomidine (DEX) on hippocampal neuron development process and on molecular expression of brain-derived neurotrophic factor (BDNF)-tyrosine receptor kinase B (TrkB) signaling pathway in neonatal rats. The hippocampal neuron cells were isolated from newborn neonatal rats and cultured in vitro. One control group and three treated groups with 1, 10, and 100 µmol/L DEX were used for the study. Cell activity and apoptosis were detected by the MTT and terminal deoxynucleotidyl transferase-mediated biotinylated uridine triphosphate (UTP) nick end labeling assays. The synaptophysin (SYN) and postsynaptic density 95 (PSD95) were detected by quantitative polymerase chain reaction. There was no difference in the viability of neuron cells among the different dose groups of DEX and the control group during days 2-10 (P>0.05). Compared to the control group, there was no significant difference (P>0.05) in the expressions of SYN and PSD95 in the groups treated with 1 and 10 µmol/L DEX, whereas significant difference in the expression was observed in the group treated with 100 µmol/L DEX (P<0.01). Compared with the control group, the expression of BDNF was significantly upregulated (P<0.05) in the group treated with 100 µmol/L DEX. There were no significant differences in TrkB expression among the four groups. The expression of p-N-methyl-D-aspartate receptor increased with an increase in the concentration of DEX; however, only the high dose revealed a significant upregulation compared with the control group. The neuroprotective effect of DEX may be achieved by upregulating the expression of BDNF and phosphorylation level of N-methyl-D-aspartate receptor. Keywords: dexmedetomidine, hippocampal neuron, development, BDNF-TrkB
format	article
author	Lv J Ou W Zou XH Yao Y Wu JL
author_facet	Lv J Ou W Zou XH Yao Y Wu JL
author_sort	Lv J
title	Effect of dexmedetomidine on hippocampal neuron development and BDNF-TrkB signal expression in neonatal rats
title_short	Effect of dexmedetomidine on hippocampal neuron development and BDNF-TrkB signal expression in neonatal rats
title_full	Effect of dexmedetomidine on hippocampal neuron development and BDNF-TrkB signal expression in neonatal rats
title_fullStr	Effect of dexmedetomidine on hippocampal neuron development and BDNF-TrkB signal expression in neonatal rats
title_full_unstemmed	Effect of dexmedetomidine on hippocampal neuron development and BDNF-TrkB signal expression in neonatal rats
title_sort	effect of dexmedetomidine on hippocampal neuron development and bdnf-trkb signal expression in neonatal rats
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/cd1acb1ecb3444dfb8b7f841d0548f91
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Effect of dexmedetomidine on hippocampal neuron development and BDNF-TrkB signal expression in neonatal rats

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