The role of FOSL1 in stem-like cell reprogramming processes

Abstract Cancer stem-like cells (CSCs) have self-renewal abilities responsible for cancer progression, therapy resistance, and metastatic growth. The glioblastoma stem-like cells are the most studied among CSC populations. A recent study identified four transcription factors (SOX2, SALL2, OLIG2, and...

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Autores principales:	Valeria Pecce, Antonella Verrienti, Giulia Fiscon, Marialuisa Sponziello, Federica Conte, Luana Abballe, Cosimo Durante, Lorenzo Farina, Sebastiano Filetti, Paola Paci
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cd1c4f27242a4ec8a9ec2d8affe631c5
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spelling	oai:doaj.org-article:cd1c4f27242a4ec8a9ec2d8affe631c52021-12-02T16:26:23ZThe role of FOSL1 in stem-like cell reprogramming processes10.1038/s41598-021-94072-02045-2322https://doaj.org/article/cd1c4f27242a4ec8a9ec2d8affe631c52021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94072-0https://doaj.org/toc/2045-2322Abstract Cancer stem-like cells (CSCs) have self-renewal abilities responsible for cancer progression, therapy resistance, and metastatic growth. The glioblastoma stem-like cells are the most studied among CSC populations. A recent study identified four transcription factors (SOX2, SALL2, OLIG2, and POU3F2) as the minimal core sufficient to reprogram differentiated glioblastoma (GBM) cells into stem-like cells. Transcriptomic data of GBM tissues and cell lines from two different datasets were then analyzed by the SWItch Miner (SWIM), a network-based software, and FOSL1 was identified as a putative regulator of the previously identified minimal core. Herein, we selected NTERA-2 and HEK293T cells to perform an in vitro study to investigate the role of FOSL1 in the reprogramming mechanisms. We transfected the two cell lines with a constitutive FOSL1 cDNA plasmid. We demonstrated that FOSL1 directly regulates the four transcription factors binding their promoter regions, is involved in the deregulation of several stemness markers, and reduces the cells’ ability to generate aggregates increasing the extracellular matrix component FN1. Although further experiments are necessary, our data suggest that FOSL1 reprograms the stemness by regulating the core of the four transcription factors.Valeria PecceAntonella VerrientiGiulia FisconMarialuisa SponzielloFederica ConteLuana AbballeCosimo DuranteLorenzo FarinaSebastiano FilettiPaola PaciNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Valeria Pecce Antonella Verrienti Giulia Fiscon Marialuisa Sponziello Federica Conte Luana Abballe Cosimo Durante Lorenzo Farina Sebastiano Filetti Paola Paci The role of FOSL1 in stem-like cell reprogramming processes
description	Abstract Cancer stem-like cells (CSCs) have self-renewal abilities responsible for cancer progression, therapy resistance, and metastatic growth. The glioblastoma stem-like cells are the most studied among CSC populations. A recent study identified four transcription factors (SOX2, SALL2, OLIG2, and POU3F2) as the minimal core sufficient to reprogram differentiated glioblastoma (GBM) cells into stem-like cells. Transcriptomic data of GBM tissues and cell lines from two different datasets were then analyzed by the SWItch Miner (SWIM), a network-based software, and FOSL1 was identified as a putative regulator of the previously identified minimal core. Herein, we selected NTERA-2 and HEK293T cells to perform an in vitro study to investigate the role of FOSL1 in the reprogramming mechanisms. We transfected the two cell lines with a constitutive FOSL1 cDNA plasmid. We demonstrated that FOSL1 directly regulates the four transcription factors binding their promoter regions, is involved in the deregulation of several stemness markers, and reduces the cells’ ability to generate aggregates increasing the extracellular matrix component FN1. Although further experiments are necessary, our data suggest that FOSL1 reprograms the stemness by regulating the core of the four transcription factors.
format	article
author	Valeria Pecce Antonella Verrienti Giulia Fiscon Marialuisa Sponziello Federica Conte Luana Abballe Cosimo Durante Lorenzo Farina Sebastiano Filetti Paola Paci
author_facet	Valeria Pecce Antonella Verrienti Giulia Fiscon Marialuisa Sponziello Federica Conte Luana Abballe Cosimo Durante Lorenzo Farina Sebastiano Filetti Paola Paci
author_sort	Valeria Pecce
title	The role of FOSL1 in stem-like cell reprogramming processes
title_short	The role of FOSL1 in stem-like cell reprogramming processes
title_full	The role of FOSL1 in stem-like cell reprogramming processes
title_fullStr	The role of FOSL1 in stem-like cell reprogramming processes
title_full_unstemmed	The role of FOSL1 in stem-like cell reprogramming processes
title_sort	role of fosl1 in stem-like cell reprogramming processes
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/cd1c4f27242a4ec8a9ec2d8affe631c5
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The role of FOSL1 in stem-like cell reprogramming processes

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