Is Response to Genotoxic Stress Similar in Populations of African and European Ancestry? A Study of Dose-Response After in vitro Irradiation
Background: Exposure to genotoxic stress such as radiation is an important public health issue affecting a large population. The necessity of analyzing cytogenetic effects of such exposure is related to the need to estimate the associated risk. Cytogenetic biological dosimetry is based on the relati...
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2021
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oai:doaj.org-article:cd20f47c95294feaaaf0222946c326ec2021-11-11T15:13:51ZIs Response to Genotoxic Stress Similar in Populations of African and European Ancestry? A Study of Dose-Response After in vitro Irradiation1664-802110.3389/fgene.2021.657999https://doaj.org/article/cd20f47c95294feaaaf0222946c326ec2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fgene.2021.657999/fullhttps://doaj.org/toc/1664-8021Background: Exposure to genotoxic stress such as radiation is an important public health issue affecting a large population. The necessity of analyzing cytogenetic effects of such exposure is related to the need to estimate the associated risk. Cytogenetic biological dosimetry is based on the relationship between the absorbed dose and the frequency of scored chromosomal aberrations. The influence of confounding factors on radiation response is a topical issue. The role of ethnicity is unclear. Here, we compared the dose-response curves obtained after irradiation of circulating lymphocytes from healthy donors of African and European ancestry.Materials and Methods: Blood samples from six Africans living in Africa, five Africans living in Europe, and five Caucasians living in Europe were exposed to various doses (0–4 Gy) of X-rays at a dose-rate of 0.1 Gy/min using an X-RAD320 irradiator. A validated cohort composed of 14 healthy Africans living in three African countries was included and blood samples were irradiated using the same protocols. Blood lymphocytes were cultured for 48 h and chromosomal aberrations scored during the first mitosis by telomere and centromere staining. The distribution of dicentric chromosomes was determined and the Kruskal-Wallis test was used to compare the dose-response curves of the two populations.Results: No spontaneous dicentric chromosomes were detected in African donors, thus establishing a very low background of unstable chromosomal aberrations relative to the European population. There was a significant difference in the dose response curves between native African and European donors. At 4 Gy, African donors showed a significantly lower frequency of dicentric chromosomes (p = 8.65 10–17), centric rings (p = 4.0310–14), and resulting double-strand-breaks (DSB) (p = 1.32 10–18) than European donors. In addition, a significant difference was found between African donors living in Europe and Africans living in Africa.Conclusion: This is the first study to demonstrate the important role of ethnic and environmental factors that may epigenetically influence the response to irradiation. It will be necessary to establish country-of-origen-specific dose response curves to practice precise and adequate biological dosimetry. This work opens new perspective for the comparison of treatments based on genotoxic agents, such as irradiation.Mamadou SoumboundouJulien DossouYossef KalagaInnocent NkengurutseIbrahima FayeAlbert GuinganiMacoura GadjiKoudbi J. YameogoHenri ZongoGora MbayeAhmadou DemMounibé DiarraRached AdjibadeCatherine DjebouSteffen JunkerNoufissa OudrhiriWilliam M. HempelAlain DieterlenEric JeandidierPatrice CardeElie El MaaloufBruno ColicchioAnnelise Bennaceur-GriscelliMichael FenechMichael FenechMichael FenechPhilippe VoisinClaire Rodriguez-LafrasseRadhia M’KacherFrontiers Media S.A.articleAfrican donorsEuropean donorsirradiationdicentric chromosometelomerescentromeresGeneticsQH426-470ENFrontiers in Genetics, Vol 12 (2021) |
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African donors European donors irradiation dicentric chromosome telomeres centromeres Genetics QH426-470 |
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African donors European donors irradiation dicentric chromosome telomeres centromeres Genetics QH426-470 Mamadou Soumboundou Julien Dossou Yossef Kalaga Innocent Nkengurutse Ibrahima Faye Albert Guingani Macoura Gadji Koudbi J. Yameogo Henri Zongo Gora Mbaye Ahmadou Dem Mounibé Diarra Rached Adjibade Catherine Djebou Steffen Junker Noufissa Oudrhiri William M. Hempel Alain Dieterlen Eric Jeandidier Patrice Carde Elie El Maalouf Bruno Colicchio Annelise Bennaceur-Griscelli Michael Fenech Michael Fenech Michael Fenech Philippe Voisin Claire Rodriguez-Lafrasse Radhia M’Kacher Is Response to Genotoxic Stress Similar in Populations of African and European Ancestry? A Study of Dose-Response After in vitro Irradiation |
| description |
Background: Exposure to genotoxic stress such as radiation is an important public health issue affecting a large population. The necessity of analyzing cytogenetic effects of such exposure is related to the need to estimate the associated risk. Cytogenetic biological dosimetry is based on the relationship between the absorbed dose and the frequency of scored chromosomal aberrations. The influence of confounding factors on radiation response is a topical issue. The role of ethnicity is unclear. Here, we compared the dose-response curves obtained after irradiation of circulating lymphocytes from healthy donors of African and European ancestry.Materials and Methods: Blood samples from six Africans living in Africa, five Africans living in Europe, and five Caucasians living in Europe were exposed to various doses (0–4 Gy) of X-rays at a dose-rate of 0.1 Gy/min using an X-RAD320 irradiator. A validated cohort composed of 14 healthy Africans living in three African countries was included and blood samples were irradiated using the same protocols. Blood lymphocytes were cultured for 48 h and chromosomal aberrations scored during the first mitosis by telomere and centromere staining. The distribution of dicentric chromosomes was determined and the Kruskal-Wallis test was used to compare the dose-response curves of the two populations.Results: No spontaneous dicentric chromosomes were detected in African donors, thus establishing a very low background of unstable chromosomal aberrations relative to the European population. There was a significant difference in the dose response curves between native African and European donors. At 4 Gy, African donors showed a significantly lower frequency of dicentric chromosomes (p = 8.65 10–17), centric rings (p = 4.0310–14), and resulting double-strand-breaks (DSB) (p = 1.32 10–18) than European donors. In addition, a significant difference was found between African donors living in Europe and Africans living in Africa.Conclusion: This is the first study to demonstrate the important role of ethnic and environmental factors that may epigenetically influence the response to irradiation. It will be necessary to establish country-of-origen-specific dose response curves to practice precise and adequate biological dosimetry. This work opens new perspective for the comparison of treatments based on genotoxic agents, such as irradiation. |
| format |
article |
| author |
Mamadou Soumboundou Julien Dossou Yossef Kalaga Innocent Nkengurutse Ibrahima Faye Albert Guingani Macoura Gadji Koudbi J. Yameogo Henri Zongo Gora Mbaye Ahmadou Dem Mounibé Diarra Rached Adjibade Catherine Djebou Steffen Junker Noufissa Oudrhiri William M. Hempel Alain Dieterlen Eric Jeandidier Patrice Carde Elie El Maalouf Bruno Colicchio Annelise Bennaceur-Griscelli Michael Fenech Michael Fenech Michael Fenech Philippe Voisin Claire Rodriguez-Lafrasse Radhia M’Kacher |
| author_facet |
Mamadou Soumboundou Julien Dossou Yossef Kalaga Innocent Nkengurutse Ibrahima Faye Albert Guingani Macoura Gadji Koudbi J. Yameogo Henri Zongo Gora Mbaye Ahmadou Dem Mounibé Diarra Rached Adjibade Catherine Djebou Steffen Junker Noufissa Oudrhiri William M. Hempel Alain Dieterlen Eric Jeandidier Patrice Carde Elie El Maalouf Bruno Colicchio Annelise Bennaceur-Griscelli Michael Fenech Michael Fenech Michael Fenech Philippe Voisin Claire Rodriguez-Lafrasse Radhia M’Kacher |
| author_sort |
Mamadou Soumboundou |
| title |
Is Response to Genotoxic Stress Similar in Populations of African and European Ancestry? A Study of Dose-Response After in vitro Irradiation |
| title_short |
Is Response to Genotoxic Stress Similar in Populations of African and European Ancestry? A Study of Dose-Response After in vitro Irradiation |
| title_full |
Is Response to Genotoxic Stress Similar in Populations of African and European Ancestry? A Study of Dose-Response After in vitro Irradiation |
| title_fullStr |
Is Response to Genotoxic Stress Similar in Populations of African and European Ancestry? A Study of Dose-Response After in vitro Irradiation |
| title_full_unstemmed |
Is Response to Genotoxic Stress Similar in Populations of African and European Ancestry? A Study of Dose-Response After in vitro Irradiation |
| title_sort |
is response to genotoxic stress similar in populations of african and european ancestry? a study of dose-response after in vitro irradiation |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/cd20f47c95294feaaaf0222946c326ec |
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