A Multiplex Fluidic Chip for Rapid Phenotypic Antibiotic Susceptibility Testing

ABSTRACT Many patients with severe infections receive inappropriate empirical treatment, and rapid detection of bacterial antibiotic susceptibility can improve clinical outcome and reduce mortality. To this end, we have developed a multiplex fluidic chip for rapid phenotypic antibiotic susceptibilit...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Pikkei Wistrand-Yuen, Christer Malmberg, Nikos Fatsis-Kavalopoulos, Moritz Lübke, Thomas Tängdén, Johan Kreuger
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	antibiotic susceptibility testing clinical isolates fluidic chip microfluidics multiplex Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/cd2109e2d337444da3fa1a0aa15063c2
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:cd2109e2d337444da3fa1a0aa15063c2
record_format	dspace
spelling	oai:doaj.org-article:cd2109e2d337444da3fa1a0aa15063c22021-11-15T15:56:57ZA Multiplex Fluidic Chip for Rapid Phenotypic Antibiotic Susceptibility Testing10.1128/mBio.03109-192150-7511https://doaj.org/article/cd2109e2d337444da3fa1a0aa15063c22020-02-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.03109-19https://doaj.org/toc/2150-7511ABSTRACT Many patients with severe infections receive inappropriate empirical treatment, and rapid detection of bacterial antibiotic susceptibility can improve clinical outcome and reduce mortality. To this end, we have developed a multiplex fluidic chip for rapid phenotypic antibiotic susceptibility testing of bacteria. A total of 21 clinical isolates of Escherichia coli, Klebsiella pneumoniae, and Staphylococcus aureus were acquired from the EUCAST Development Laboratory and tested against amikacin, ceftazidime, and meropenem (Gram-negative bacteria) or gentamicin, ofloxacin, and tetracycline (Gram-positive bacteria). The bacterial samples were mixed with agarose and loaded in an array of growth chambers in the chip where bacterial microcolony growth was monitored over time using automated image analysis. MIC values were automatically obtained by tracking the growth rates of individual microcolonies in different regions of antibiotic gradients. Stable MIC values were obtained within 2 to 4 h, and the results showed categorical agreement with reference MIC values as determined by broth microdilution in 86% of the cases. IMPORTANCE Prompt and effective antimicrobial therapy is crucial for the management of patients with severe bacterial infections but is becoming increasingly difficult to provide due to emerging antibiotic resistance. The traditional methods for antibiotic susceptibility testing (AST) used in most clinical laboratories are reliable but slow with turnaround times of 2 to 3 days, which necessitates the use of empirical therapy with broad-spectrum antibiotics. There is a great need for fast and reliable AST methods that enable starting targeted treatment within a few hours to improve patient outcome and reduce the overuse of broad-spectrum antibiotics. The multiplex fluidic chip for phenotypic AST described in the present study may enable data on antimicrobial resistance within 2 to 4 h, allowing for an early initiation of appropriate antibiotic therapy.Pikkei Wistrand-YuenChrister MalmbergNikos Fatsis-KavalopoulosMoritz LübkeThomas TängdénJohan KreugerAmerican Society for Microbiologyarticleantibiotic susceptibility testingclinical isolatesfluidic chipmicrofluidicsmultiplexMicrobiologyQR1-502ENmBio, Vol 11, Iss 1 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	antibiotic susceptibility testing clinical isolates fluidic chip microfluidics multiplex Microbiology QR1-502
spellingShingle	antibiotic susceptibility testing clinical isolates fluidic chip microfluidics multiplex Microbiology QR1-502 Pikkei Wistrand-Yuen Christer Malmberg Nikos Fatsis-Kavalopoulos Moritz Lübke Thomas Tängdén Johan Kreuger A Multiplex Fluidic Chip for Rapid Phenotypic Antibiotic Susceptibility Testing
description	ABSTRACT Many patients with severe infections receive inappropriate empirical treatment, and rapid detection of bacterial antibiotic susceptibility can improve clinical outcome and reduce mortality. To this end, we have developed a multiplex fluidic chip for rapid phenotypic antibiotic susceptibility testing of bacteria. A total of 21 clinical isolates of Escherichia coli, Klebsiella pneumoniae, and Staphylococcus aureus were acquired from the EUCAST Development Laboratory and tested against amikacin, ceftazidime, and meropenem (Gram-negative bacteria) or gentamicin, ofloxacin, and tetracycline (Gram-positive bacteria). The bacterial samples were mixed with agarose and loaded in an array of growth chambers in the chip where bacterial microcolony growth was monitored over time using automated image analysis. MIC values were automatically obtained by tracking the growth rates of individual microcolonies in different regions of antibiotic gradients. Stable MIC values were obtained within 2 to 4 h, and the results showed categorical agreement with reference MIC values as determined by broth microdilution in 86% of the cases. IMPORTANCE Prompt and effective antimicrobial therapy is crucial for the management of patients with severe bacterial infections but is becoming increasingly difficult to provide due to emerging antibiotic resistance. The traditional methods for antibiotic susceptibility testing (AST) used in most clinical laboratories are reliable but slow with turnaround times of 2 to 3 days, which necessitates the use of empirical therapy with broad-spectrum antibiotics. There is a great need for fast and reliable AST methods that enable starting targeted treatment within a few hours to improve patient outcome and reduce the overuse of broad-spectrum antibiotics. The multiplex fluidic chip for phenotypic AST described in the present study may enable data on antimicrobial resistance within 2 to 4 h, allowing for an early initiation of appropriate antibiotic therapy.
format	article
author	Pikkei Wistrand-Yuen Christer Malmberg Nikos Fatsis-Kavalopoulos Moritz Lübke Thomas Tängdén Johan Kreuger
author_facet	Pikkei Wistrand-Yuen Christer Malmberg Nikos Fatsis-Kavalopoulos Moritz Lübke Thomas Tängdén Johan Kreuger
author_sort	Pikkei Wistrand-Yuen
title	A Multiplex Fluidic Chip for Rapid Phenotypic Antibiotic Susceptibility Testing
title_short	A Multiplex Fluidic Chip for Rapid Phenotypic Antibiotic Susceptibility Testing
title_full	A Multiplex Fluidic Chip for Rapid Phenotypic Antibiotic Susceptibility Testing
title_fullStr	A Multiplex Fluidic Chip for Rapid Phenotypic Antibiotic Susceptibility Testing
title_full_unstemmed	A Multiplex Fluidic Chip for Rapid Phenotypic Antibiotic Susceptibility Testing
title_sort	multiplex fluidic chip for rapid phenotypic antibiotic susceptibility testing
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/cd2109e2d337444da3fa1a0aa15063c2
work_keys_str_mv	AT pikkeiwistrandyuen amultiplexfluidicchipforrapidphenotypicantibioticsusceptibilitytesting AT christermalmberg amultiplexfluidicchipforrapidphenotypicantibioticsusceptibilitytesting AT nikosfatsiskavalopoulos amultiplexfluidicchipforrapidphenotypicantibioticsusceptibilitytesting AT moritzlubke amultiplexfluidicchipforrapidphenotypicantibioticsusceptibilitytesting AT thomastangden amultiplexfluidicchipforrapidphenotypicantibioticsusceptibilitytesting AT johankreuger amultiplexfluidicchipforrapidphenotypicantibioticsusceptibilitytesting AT pikkeiwistrandyuen multiplexfluidicchipforrapidphenotypicantibioticsusceptibilitytesting AT christermalmberg multiplexfluidicchipforrapidphenotypicantibioticsusceptibilitytesting AT nikosfatsiskavalopoulos multiplexfluidicchipforrapidphenotypicantibioticsusceptibilitytesting AT moritzlubke multiplexfluidicchipforrapidphenotypicantibioticsusceptibilitytesting AT thomastangden multiplexfluidicchipforrapidphenotypicantibioticsusceptibilitytesting AT johankreuger multiplexfluidicchipforrapidphenotypicantibioticsusceptibilitytesting
_version_	1718427120658546688

A Multiplex Fluidic Chip for Rapid Phenotypic Antibiotic Susceptibility Testing

Ejemplares similares