Protective effect of Lactobacillus-containing probiotics on intestinal mucosa of rats experiencing traumatic hemorrhagic shock

This study was conducted to assess whether Lactobacillus-containing probiotics could protect intestinal mucosa in rats during traumatic hemorrhagic shock and to determine its underlying mechanisms. Healthy male Sprague–Dawley rats (300 ± 20 g) were randomly divided into four groups. During the study...

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Autores principales:	Wang Lei, Liu Shu-li, Xu Zhi-peng, Song Qi, Li Lei, Qiu Zhao-lei, Wang Zhen-jie
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	compound lactobacillus trauma mucosal integrity nf-κb signaling pathway Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/cd21fca2d5fc4e1d8a39ca4fb416f7c6
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spelling	oai:doaj.org-article:cd21fca2d5fc4e1d8a39ca4fb416f7c62021-12-05T14:10:42ZProtective effect of Lactobacillus-containing probiotics on intestinal mucosa of rats experiencing traumatic hemorrhagic shock2391-541210.1515/biol-2021-0112https://doaj.org/article/cd21fca2d5fc4e1d8a39ca4fb416f7c62021-10-01T00:00:00Zhttps://doi.org/10.1515/biol-2021-0112https://doaj.org/toc/2391-5412This study was conducted to assess whether Lactobacillus-containing probiotics could protect intestinal mucosa in rats during traumatic hemorrhagic shock and to determine its underlying mechanisms. Healthy male Sprague–Dawley rats (300 ± 20 g) were randomly divided into four groups. During the study, reverse transcription polymerase chain reaction, western blotting, and hematoxylin and eosin methods were used. There was a significant increase in the expression of toll-like receptor 4 (TLR4) in the rats that experienced traumatic hemorrhagic shock, along with increased mRNA of tumor necrosis factor-alpha (TNF-α) and interleukin (IL)-6. Pretreatment with Lactobacillus-containing probiotics reduced TLR4 expression, decreased phosphorylation (Ser536) and acetylation (Lys310) of p65, and decreased TNF-α and IL-6 mRNA. The probiotics combined acetate Ringer’s group showed a less severe pathological manifestation compared to the other experimental groups. Lactobacillus-containing probiotics inhibited nuclear factor-kappa B signaling via the downregulation of TLR4, resulting in inflammatory homeostasis, which might be the mechanism whereby Lactobacillus protects the intestinal mucosa from damage caused by the traumatic hemorrhagic shock.Wang LeiLiu Shu-liXu Zhi-pengSong QiLi LeiQiu Zhao-leiWang Zhen-jieDe Gruyterarticlecompound lactobacillus traumamucosal integritynf-κb signaling pathwayBiology (General)QH301-705.5ENOpen Life Sciences, Vol 16, Iss 1, Pp 1122-1129 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	compound lactobacillus trauma mucosal integrity nf-κb signaling pathway Biology (General) QH301-705.5
spellingShingle	compound lactobacillus trauma mucosal integrity nf-κb signaling pathway Biology (General) QH301-705.5 Wang Lei Liu Shu-li Xu Zhi-peng Song Qi Li Lei Qiu Zhao-lei Wang Zhen-jie Protective effect of Lactobacillus-containing probiotics on intestinal mucosa of rats experiencing traumatic hemorrhagic shock
description	This study was conducted to assess whether Lactobacillus-containing probiotics could protect intestinal mucosa in rats during traumatic hemorrhagic shock and to determine its underlying mechanisms. Healthy male Sprague–Dawley rats (300 ± 20 g) were randomly divided into four groups. During the study, reverse transcription polymerase chain reaction, western blotting, and hematoxylin and eosin methods were used. There was a significant increase in the expression of toll-like receptor 4 (TLR4) in the rats that experienced traumatic hemorrhagic shock, along with increased mRNA of tumor necrosis factor-alpha (TNF-α) and interleukin (IL)-6. Pretreatment with Lactobacillus-containing probiotics reduced TLR4 expression, decreased phosphorylation (Ser536) and acetylation (Lys310) of p65, and decreased TNF-α and IL-6 mRNA. The probiotics combined acetate Ringer’s group showed a less severe pathological manifestation compared to the other experimental groups. Lactobacillus-containing probiotics inhibited nuclear factor-kappa B signaling via the downregulation of TLR4, resulting in inflammatory homeostasis, which might be the mechanism whereby Lactobacillus protects the intestinal mucosa from damage caused by the traumatic hemorrhagic shock.
format	article
author	Wang Lei Liu Shu-li Xu Zhi-peng Song Qi Li Lei Qiu Zhao-lei Wang Zhen-jie
author_facet	Wang Lei Liu Shu-li Xu Zhi-peng Song Qi Li Lei Qiu Zhao-lei Wang Zhen-jie
author_sort	Wang Lei
title	Protective effect of Lactobacillus-containing probiotics on intestinal mucosa of rats experiencing traumatic hemorrhagic shock
title_short	Protective effect of Lactobacillus-containing probiotics on intestinal mucosa of rats experiencing traumatic hemorrhagic shock
title_full	Protective effect of Lactobacillus-containing probiotics on intestinal mucosa of rats experiencing traumatic hemorrhagic shock
title_fullStr	Protective effect of Lactobacillus-containing probiotics on intestinal mucosa of rats experiencing traumatic hemorrhagic shock
title_full_unstemmed	Protective effect of Lactobacillus-containing probiotics on intestinal mucosa of rats experiencing traumatic hemorrhagic shock
title_sort	protective effect of lactobacillus-containing probiotics on intestinal mucosa of rats experiencing traumatic hemorrhagic shock
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/cd21fca2d5fc4e1d8a39ca4fb416f7c6
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Protective effect of Lactobacillus-containing probiotics on intestinal mucosa of rats experiencing traumatic hemorrhagic shock

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