Novel Cellulose Fibre-Based Flexible Plasmonic Membrane for Point-of-Care SERS Biomarker Detection in Chronic Wound Healing

Jayakumar Perumal,1 Hann Qian Lim,1 Amalina Binte Ebrahim Attia,1 Riazul Raziq,1 David Ian Leavesley,2 Zee Upton,2 US Dinish,1 Malini Olivo1 1Translational Biophotonics Laboratory, Institute of Bioengineering and Bioimaging, Agency for Science Technology and Research (A*STAR), Singapore; 2Skin Resea...

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Autores principales:	Perumal J, Lim HQ, Attia ABE, Raziq R, Leavesley DI, Upton Z, Dinish US, Olivo M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	sers cellulose fibres membrane sers point-of-care paper devices wound biomarkers flexible biosensor Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/cd2429449eb4464a8ec6d02228c785fc
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spelling	oai:doaj.org-article:cd2429449eb4464a8ec6d02228c785fc2021-12-02T18:45:38ZNovel Cellulose Fibre-Based Flexible Plasmonic Membrane for Point-of-Care SERS Biomarker Detection in Chronic Wound Healing1178-2013https://doaj.org/article/cd2429449eb4464a8ec6d02228c785fc2021-08-01T00:00:00Zhttps://www.dovepress.com/novel-cellulose-fibre-based-flexible-plasmonic-membrane-for-point-of-c-peer-reviewed-fulltext-article-IJNhttps://doaj.org/toc/1178-2013Jayakumar Perumal,1 Hann Qian Lim,1 Amalina Binte Ebrahim Attia,1 Riazul Raziq,1 David Ian Leavesley,2 Zee Upton,2 US Dinish,1 Malini Olivo1 1Translational Biophotonics Laboratory, Institute of Bioengineering and Bioimaging, Agency for Science Technology and Research (ASTAR), Singapore; 2Skin Research Institute of Singapore, Agency for Science, Technology and Research (ASTAR), SingaporeCorrespondence: Malini OlivoTranslational Biophotonics Laboratory, Institute of Bioengineering and Bioimaging, Agency for Science Technology and Research (A*STAR), SingaporeEmail Malini_Olivo@sbic.a-star.edu.sgBackground: Wound management is stretching the limits of health systems globally, challenging clinicians to evaluate the effectiveness of their treatments and deliver appropriate care to their patients. Visual inspection and manual measurement of wound size are subjective, often inaccurate and inconsistent. Growth factors, such as pro-inflammatory cytokines and proteases, play important roles in cutaneous wound healing. However, little is known about the point-of-care monitoring of the changes in such markers during the healing process. Here, we explore the capability of surface-enhanced Raman spectroscopy (SERS) as a viable point-of-care platform to monitor the changes of these surrogate indicators of healing status in chronic wounds.Methods: We developed a biofunctionalized flexible, cost-effective, scalable and easy-to-fabricate plasmonic SERS substrate using cellulose fibre (CF), which is used for sensing of wound markers based on a modified immunoassay method.Results: We evaluated and selected the reliable silver nano-island thickness that will be sputtered onto the CF-based substrate for the highest SERS enhancement. Using this biofunctionalized SERS substrate, we detected varying concentrations of MMP-9 (10– 5000 ng/mL) and TNF-α (5– 100 ng/mL) proteins to model the wound exudates. This SERS detection method demonstrates a linear response within biologically relevant concentrations, ranging from 10 to 500 ng/mL for MMP-9 and 5 to 25 ng/mL for TNF-α for these surrogate indicators.Conclusion: Our SERS sensing platform achieved detection limits in the μM to sub-nM range and displayed high sensitivity and selectivity. This could result in a cheap, point-of-care device that provides a non-invasive measure of cutaneous wound healing in real time. We envision that these flexible substrates after activation may be incorporated into wound dressings in future for routine monitoring of wound healing status.Keywords: SERS, cellulose fibres, membrane SERS, point-of-care, paper devices, wound biomarkers, flexible biosensorPerumal JLim HQAttia ABERaziq RLeavesley DIUpton ZDinish USOlivo MDove Medical Pressarticleserscellulose fibresmembrane serspoint-of-carepaper deviceswound biomarkersflexible biosensorMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 16, Pp 5869-5878 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	sers cellulose fibres membrane sers point-of-care paper devices wound biomarkers flexible biosensor Medicine (General) R5-920
spellingShingle	sers cellulose fibres membrane sers point-of-care paper devices wound biomarkers flexible biosensor Medicine (General) R5-920 Perumal J Lim HQ Attia ABE Raziq R Leavesley DI Upton Z Dinish US Olivo M Novel Cellulose Fibre-Based Flexible Plasmonic Membrane for Point-of-Care SERS Biomarker Detection in Chronic Wound Healing
description	Jayakumar Perumal,1 Hann Qian Lim,1 Amalina Binte Ebrahim Attia,1 Riazul Raziq,1 David Ian Leavesley,2 Zee Upton,2 US Dinish,1 Malini Olivo1 1Translational Biophotonics Laboratory, Institute of Bioengineering and Bioimaging, Agency for Science Technology and Research (ASTAR), Singapore; 2Skin Research Institute of Singapore, Agency for Science, Technology and Research (ASTAR), SingaporeCorrespondence: Malini OlivoTranslational Biophotonics Laboratory, Institute of Bioengineering and Bioimaging, Agency for Science Technology and Research (A*STAR), SingaporeEmail Malini_Olivo@sbic.a-star.edu.sgBackground: Wound management is stretching the limits of health systems globally, challenging clinicians to evaluate the effectiveness of their treatments and deliver appropriate care to their patients. Visual inspection and manual measurement of wound size are subjective, often inaccurate and inconsistent. Growth factors, such as pro-inflammatory cytokines and proteases, play important roles in cutaneous wound healing. However, little is known about the point-of-care monitoring of the changes in such markers during the healing process. Here, we explore the capability of surface-enhanced Raman spectroscopy (SERS) as a viable point-of-care platform to monitor the changes of these surrogate indicators of healing status in chronic wounds.Methods: We developed a biofunctionalized flexible, cost-effective, scalable and easy-to-fabricate plasmonic SERS substrate using cellulose fibre (CF), which is used for sensing of wound markers based on a modified immunoassay method.Results: We evaluated and selected the reliable silver nano-island thickness that will be sputtered onto the CF-based substrate for the highest SERS enhancement. Using this biofunctionalized SERS substrate, we detected varying concentrations of MMP-9 (10– 5000 ng/mL) and TNF-α (5– 100 ng/mL) proteins to model the wound exudates. This SERS detection method demonstrates a linear response within biologically relevant concentrations, ranging from 10 to 500 ng/mL for MMP-9 and 5 to 25 ng/mL for TNF-α for these surrogate indicators.Conclusion: Our SERS sensing platform achieved detection limits in the μM to sub-nM range and displayed high sensitivity and selectivity. This could result in a cheap, point-of-care device that provides a non-invasive measure of cutaneous wound healing in real time. We envision that these flexible substrates after activation may be incorporated into wound dressings in future for routine monitoring of wound healing status.Keywords: SERS, cellulose fibres, membrane SERS, point-of-care, paper devices, wound biomarkers, flexible biosensor
format	article
author	Perumal J Lim HQ Attia ABE Raziq R Leavesley DI Upton Z Dinish US Olivo M
author_facet	Perumal J Lim HQ Attia ABE Raziq R Leavesley DI Upton Z Dinish US Olivo M
author_sort	Perumal J
title	Novel Cellulose Fibre-Based Flexible Plasmonic Membrane for Point-of-Care SERS Biomarker Detection in Chronic Wound Healing
title_short	Novel Cellulose Fibre-Based Flexible Plasmonic Membrane for Point-of-Care SERS Biomarker Detection in Chronic Wound Healing
title_full	Novel Cellulose Fibre-Based Flexible Plasmonic Membrane for Point-of-Care SERS Biomarker Detection in Chronic Wound Healing
title_fullStr	Novel Cellulose Fibre-Based Flexible Plasmonic Membrane for Point-of-Care SERS Biomarker Detection in Chronic Wound Healing
title_full_unstemmed	Novel Cellulose Fibre-Based Flexible Plasmonic Membrane for Point-of-Care SERS Biomarker Detection in Chronic Wound Healing
title_sort	novel cellulose fibre-based flexible plasmonic membrane for point-of-care sers biomarker detection in chronic wound healing
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/cd2429449eb4464a8ec6d02228c785fc
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Novel Cellulose Fibre-Based Flexible Plasmonic Membrane for Point-of-Care SERS Biomarker Detection in Chronic Wound Healing

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