An activated‐zinc oral rinse reduces pro‐inflammatory cytokine secretion and promotes proliferation in Porphyromonas gingivalis LPS‐challenged gingival tissues – A pilot study

Abstract Objectives The use of adjunct post‐treatment mouth rinses containing chlorhexidine (CHX) for periodontitis patients is associated with side effects that reduce patient compliance. Our aim was to evaluate the proinflammatory and cell proliferation effects of an activated‐zinc mouth rinse (SM...

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Autores principales:	Kaitlyn A. Stanton, Barbara A. McCracken
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	immunohistochemistry patient compliance periodontitis Dentistry RK1-715
Acceso en línea:	https://doaj.org/article/cd2a02ec4b224791814b71869ad449d8
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spelling	oai:doaj.org-article:cd2a02ec4b224791814b71869ad449d82021-12-02T12:59:21ZAn activated‐zinc oral rinse reduces pro‐inflammatory cytokine secretion and promotes proliferation in Porphyromonas gingivalis LPS‐challenged gingival tissues – A pilot study2057-434710.1002/cre2.437https://doaj.org/article/cd2a02ec4b224791814b71869ad449d82021-12-01T00:00:00Zhttps://doi.org/10.1002/cre2.437https://doaj.org/toc/2057-4347Abstract Objectives The use of adjunct post‐treatment mouth rinses containing chlorhexidine (CHX) for periodontitis patients is associated with side effects that reduce patient compliance. Our aim was to evaluate the proinflammatory and cell proliferation effects of an activated‐zinc mouth rinse (SM) that has been suggested as an alternative post‐treatment therapeutic. Materials and Methods Tissue models of gingival epithelium were used to simulate periodontal disease and compare inflammatory reactions after treatment with CHX or SM. Tissues were exposed to Porphyromonas gingivalis LPS and wounded to simulate periodontal disease. Tissues were treated and incubated for 6, 12, or 24 h. Inflammatory cytokines were measured in culture medium by ELISA and local expression of Toll‐like receptor (TLR)‐4 and proliferation marker Ki‐67 was visualized by immunohistochemistry. Results SM and CHX treatments decreased secretion of IL‐1β and IL‐8 into culture media at all time points. IL‐1β secretion levels were further decreased by SM compared to CHX treatment at all time points. TLR‐4 expression appeared significantly increased 12 h post‐treatment in the CHX tissues but remained relatively low in SM tissues at all time points. Ki‐67 results suggest that cell proliferation was increased in the SM tissues earlier than CHX tissues. Conclusions Our data suggest that SM may reduce inflammation in gingival tissues.Kaitlyn A. StantonBarbara A. McCrackenWileyarticleimmunohistochemistrypatient complianceperiodontitisDentistryRK1-715ENClinical and Experimental Dental Research, Vol 7, Iss 6, Pp 995-1001 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	immunohistochemistry patient compliance periodontitis Dentistry RK1-715
spellingShingle	immunohistochemistry patient compliance periodontitis Dentistry RK1-715 Kaitlyn A. Stanton Barbara A. McCracken An activated‐zinc oral rinse reduces pro‐inflammatory cytokine secretion and promotes proliferation in Porphyromonas gingivalis LPS‐challenged gingival tissues – A pilot study
description	Abstract Objectives The use of adjunct post‐treatment mouth rinses containing chlorhexidine (CHX) for periodontitis patients is associated with side effects that reduce patient compliance. Our aim was to evaluate the proinflammatory and cell proliferation effects of an activated‐zinc mouth rinse (SM) that has been suggested as an alternative post‐treatment therapeutic. Materials and Methods Tissue models of gingival epithelium were used to simulate periodontal disease and compare inflammatory reactions after treatment with CHX or SM. Tissues were exposed to Porphyromonas gingivalis LPS and wounded to simulate periodontal disease. Tissues were treated and incubated for 6, 12, or 24 h. Inflammatory cytokines were measured in culture medium by ELISA and local expression of Toll‐like receptor (TLR)‐4 and proliferation marker Ki‐67 was visualized by immunohistochemistry. Results SM and CHX treatments decreased secretion of IL‐1β and IL‐8 into culture media at all time points. IL‐1β secretion levels were further decreased by SM compared to CHX treatment at all time points. TLR‐4 expression appeared significantly increased 12 h post‐treatment in the CHX tissues but remained relatively low in SM tissues at all time points. Ki‐67 results suggest that cell proliferation was increased in the SM tissues earlier than CHX tissues. Conclusions Our data suggest that SM may reduce inflammation in gingival tissues.
format	article
author	Kaitlyn A. Stanton Barbara A. McCracken
author_facet	Kaitlyn A. Stanton Barbara A. McCracken
author_sort	Kaitlyn A. Stanton
title	An activated‐zinc oral rinse reduces pro‐inflammatory cytokine secretion and promotes proliferation in Porphyromonas gingivalis LPS‐challenged gingival tissues – A pilot study
title_short	An activated‐zinc oral rinse reduces pro‐inflammatory cytokine secretion and promotes proliferation in Porphyromonas gingivalis LPS‐challenged gingival tissues – A pilot study
title_full	An activated‐zinc oral rinse reduces pro‐inflammatory cytokine secretion and promotes proliferation in Porphyromonas gingivalis LPS‐challenged gingival tissues – A pilot study
title_fullStr	An activated‐zinc oral rinse reduces pro‐inflammatory cytokine secretion and promotes proliferation in Porphyromonas gingivalis LPS‐challenged gingival tissues – A pilot study
title_full_unstemmed	An activated‐zinc oral rinse reduces pro‐inflammatory cytokine secretion and promotes proliferation in Porphyromonas gingivalis LPS‐challenged gingival tissues – A pilot study
title_sort	activated‐zinc oral rinse reduces pro‐inflammatory cytokine secretion and promotes proliferation in porphyromonas gingivalis lps‐challenged gingival tissues – a pilot study
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/cd2a02ec4b224791814b71869ad449d8
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An activated‐zinc oral rinse reduces pro‐inflammatory cytokine secretion and promotes proliferation in Porphyromonas gingivalis LPS‐challenged gingival tissues – A pilot study

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