Comparison of nasal and bronchial epithelial cells obtained from patients with COPD.

For in vitro studies of airway pathophysiology, primary epithelial cells have many advantages over immortalised cell lines. Nasal epithelial cells are easier to obtain than bronchial epithelial cells and can be used as an alternative for in vitro studies. Our objective was to compare nasal and bronc...

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Autores principales:	David M Comer, J Stuart Elborn, Madeleine Ennis
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cd2c623ee73543868f9832c1a76c8272
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id	oai:doaj.org-article:cd2c623ee73543868f9832c1a76c8272
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spelling	oai:doaj.org-article:cd2c623ee73543868f9832c1a76c82722021-11-18T07:25:56ZComparison of nasal and bronchial epithelial cells obtained from patients with COPD.1932-620310.1371/journal.pone.0032924https://doaj.org/article/cd2c623ee73543868f9832c1a76c82722012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22412951/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203For in vitro studies of airway pathophysiology, primary epithelial cells have many advantages over immortalised cell lines. Nasal epithelial cells are easier to obtain than bronchial epithelial cells and can be used as an alternative for in vitro studies. Our objective was to compare nasal and bronchial epithelial cells from subjects with COPD to establish if these cells respond similarly to pro-inflammatory stimuli. Cell cultures from paired nasal and bronchial brushings (21 subjects) were incubated with cigarette smoke extract (CSE) prior to stimulation with Pseudomonas aeruginosa lipopolysaccharide. IL-6 and IL-8 were measured by ELISA and Toll-like receptor 4 (TLR-4) message and expression by RT-PCR and FACS respectively. IL-8 release correlated significantly between the two cell types. IL-6 secretion was significantly less from bronchial compared to nasal epithelial cells and secreted concentrations did not correlate. A 4 h CSE incubation was immunosuppressive for both nasal and bronchial cells, however prolonged incubation for 24 h was pro-inflammatory solely for the nasal cells. CSE reduced TLR-4 expression in bronchial cells only after 24 h, and was without effect on mRNA expression. In subjects with COPD, nasal epithelial cells cannot substitute for in vitro bronchial epithelial cells in airway inflammation studies.David M ComerJ Stuart ElbornMadeleine EnnisPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 3, p e32924 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q David M Comer J Stuart Elborn Madeleine Ennis Comparison of nasal and bronchial epithelial cells obtained from patients with COPD.
description	For in vitro studies of airway pathophysiology, primary epithelial cells have many advantages over immortalised cell lines. Nasal epithelial cells are easier to obtain than bronchial epithelial cells and can be used as an alternative for in vitro studies. Our objective was to compare nasal and bronchial epithelial cells from subjects with COPD to establish if these cells respond similarly to pro-inflammatory stimuli. Cell cultures from paired nasal and bronchial brushings (21 subjects) were incubated with cigarette smoke extract (CSE) prior to stimulation with Pseudomonas aeruginosa lipopolysaccharide. IL-6 and IL-8 were measured by ELISA and Toll-like receptor 4 (TLR-4) message and expression by RT-PCR and FACS respectively. IL-8 release correlated significantly between the two cell types. IL-6 secretion was significantly less from bronchial compared to nasal epithelial cells and secreted concentrations did not correlate. A 4 h CSE incubation was immunosuppressive for both nasal and bronchial cells, however prolonged incubation for 24 h was pro-inflammatory solely for the nasal cells. CSE reduced TLR-4 expression in bronchial cells only after 24 h, and was without effect on mRNA expression. In subjects with COPD, nasal epithelial cells cannot substitute for in vitro bronchial epithelial cells in airway inflammation studies.
format	article
author	David M Comer J Stuart Elborn Madeleine Ennis
author_facet	David M Comer J Stuart Elborn Madeleine Ennis
author_sort	David M Comer
title	Comparison of nasal and bronchial epithelial cells obtained from patients with COPD.
title_short	Comparison of nasal and bronchial epithelial cells obtained from patients with COPD.
title_full	Comparison of nasal and bronchial epithelial cells obtained from patients with COPD.
title_fullStr	Comparison of nasal and bronchial epithelial cells obtained from patients with COPD.
title_full_unstemmed	Comparison of nasal and bronchial epithelial cells obtained from patients with COPD.
title_sort	comparison of nasal and bronchial epithelial cells obtained from patients with copd.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/cd2c623ee73543868f9832c1a76c8272
work_keys_str_mv	AT davidmcomer comparisonofnasalandbronchialepithelialcellsobtainedfrompatientswithcopd AT jstuartelborn comparisonofnasalandbronchialepithelialcellsobtainedfrompatientswithcopd AT madeleineennis comparisonofnasalandbronchialepithelialcellsobtainedfrompatientswithcopd
_version_	1718423500351340544

Comparison of nasal and bronchial epithelial cells obtained from patients with COPD.

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