Morphology, structure and function characterization of PEI modified magnetic nanoparticles gene delivery system.

Modified magnetic nanoparticles are used as non-viral gene carriers in biological applications. To achieve successful gene delivery, it is critical that nanoparticles effectually assemble with nucleic acids. However, relatively little work has been conducted on the assemble mechanisms between nanopa...

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Autores principales:	Xiang Zhao, Haixin Cui, Wenjie Chen, Yan Wang, Bo Cui, Changjiao Sun, Zhigang Meng, Guoqiang Liu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cd3d5b1f743c42e798b9c88a469584b5
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spelling	oai:doaj.org-article:cd3d5b1f743c42e798b9c88a469584b52021-11-18T08:16:34ZMorphology, structure and function characterization of PEI modified magnetic nanoparticles gene delivery system.1932-620310.1371/journal.pone.0098919https://doaj.org/article/cd3d5b1f743c42e798b9c88a469584b52014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24911360/?tool=EBIhttps://doaj.org/toc/1932-6203Modified magnetic nanoparticles are used as non-viral gene carriers in biological applications. To achieve successful gene delivery, it is critical that nanoparticles effectually assemble with nucleic acids. However, relatively little work has been conducted on the assemble mechanisms between nanoparticles and DNA, and its effects on transfection efficiency. Using biophysical and biochemical characterization, along with Atomic force microscopy (AFM) and Transmission electron microscopy (TEM), we investigate the morphologies, assembling structures and gene delivering abilities of the PEI modified magnetic nanoparticles (MNPs) gene delivery system. In this gene delivery system, MNP/DNA complexes are formed via binding of DNA onto the surface of MNPs. MNPs are favorable to not only increase DNA concentration but also prevent DNA degradation. Magnetofection experiments showed that MNPs has low cytotoxicity and introduces highly stable transfection in mammalian somatic cells. In addition, different binding ratios between MNPs and DNA result in various morphologies of MNP/DNA complexes and have an influence on transfection efficiency. Dose-response profile indicated that transfection efficiency positively correlate with MNP/DNA ratio. Furthermore, intracellular tracking demonstrate that MNPs move though the cell membranes, deliver and release exogenous DNA into the nucleus.Xiang ZhaoHaixin CuiWenjie ChenYan WangBo CuiChangjiao SunZhigang MengGuoqiang LiuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 6, p e98919 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiang Zhao Haixin Cui Wenjie Chen Yan Wang Bo Cui Changjiao Sun Zhigang Meng Guoqiang Liu Morphology, structure and function characterization of PEI modified magnetic nanoparticles gene delivery system.
description	Modified magnetic nanoparticles are used as non-viral gene carriers in biological applications. To achieve successful gene delivery, it is critical that nanoparticles effectually assemble with nucleic acids. However, relatively little work has been conducted on the assemble mechanisms between nanoparticles and DNA, and its effects on transfection efficiency. Using biophysical and biochemical characterization, along with Atomic force microscopy (AFM) and Transmission electron microscopy (TEM), we investigate the morphologies, assembling structures and gene delivering abilities of the PEI modified magnetic nanoparticles (MNPs) gene delivery system. In this gene delivery system, MNP/DNA complexes are formed via binding of DNA onto the surface of MNPs. MNPs are favorable to not only increase DNA concentration but also prevent DNA degradation. Magnetofection experiments showed that MNPs has low cytotoxicity and introduces highly stable transfection in mammalian somatic cells. In addition, different binding ratios between MNPs and DNA result in various morphologies of MNP/DNA complexes and have an influence on transfection efficiency. Dose-response profile indicated that transfection efficiency positively correlate with MNP/DNA ratio. Furthermore, intracellular tracking demonstrate that MNPs move though the cell membranes, deliver and release exogenous DNA into the nucleus.
format	article
author	Xiang Zhao Haixin Cui Wenjie Chen Yan Wang Bo Cui Changjiao Sun Zhigang Meng Guoqiang Liu
author_facet	Xiang Zhao Haixin Cui Wenjie Chen Yan Wang Bo Cui Changjiao Sun Zhigang Meng Guoqiang Liu
author_sort	Xiang Zhao
title	Morphology, structure and function characterization of PEI modified magnetic nanoparticles gene delivery system.
title_short	Morphology, structure and function characterization of PEI modified magnetic nanoparticles gene delivery system.
title_full	Morphology, structure and function characterization of PEI modified magnetic nanoparticles gene delivery system.
title_fullStr	Morphology, structure and function characterization of PEI modified magnetic nanoparticles gene delivery system.
title_full_unstemmed	Morphology, structure and function characterization of PEI modified magnetic nanoparticles gene delivery system.
title_sort	morphology, structure and function characterization of pei modified magnetic nanoparticles gene delivery system.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/cd3d5b1f743c42e798b9c88a469584b5
work_keys_str_mv	AT xiangzhao morphologystructureandfunctioncharacterizationofpeimodifiedmagneticnanoparticlesgenedeliverysystem AT haixincui morphologystructureandfunctioncharacterizationofpeimodifiedmagneticnanoparticlesgenedeliverysystem AT wenjiechen morphologystructureandfunctioncharacterizationofpeimodifiedmagneticnanoparticlesgenedeliverysystem AT yanwang morphologystructureandfunctioncharacterizationofpeimodifiedmagneticnanoparticlesgenedeliverysystem AT bocui morphologystructureandfunctioncharacterizationofpeimodifiedmagneticnanoparticlesgenedeliverysystem AT changjiaosun morphologystructureandfunctioncharacterizationofpeimodifiedmagneticnanoparticlesgenedeliverysystem AT zhigangmeng morphologystructureandfunctioncharacterizationofpeimodifiedmagneticnanoparticlesgenedeliverysystem AT guoqiangliu morphologystructureandfunctioncharacterizationofpeimodifiedmagneticnanoparticlesgenedeliverysystem
_version_	1718422012952576000

Morphology, structure and function characterization of PEI modified magnetic nanoparticles gene delivery system.

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