Immunoinformatic based identification of cytotoxic T lymphocyte epitopes from the Indian isolate of SARS-CoV-2

Abstract The Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has turned into a pandemic with about thirty million confirmed cases worldwide as of September 2020. Being an airborne infection, it can be catastrophic to populous countries like India. This study sets to identify potential c...

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Autores principales:	Viswajit Mulpuru, Nidhi Mishra
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cd407f3a78dd4c5a9fc902f77a9ce81e
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spelling	oai:doaj.org-article:cd407f3a78dd4c5a9fc902f77a9ce81e2021-12-02T13:19:21ZImmunoinformatic based identification of cytotoxic T lymphocyte epitopes from the Indian isolate of SARS-CoV-210.1038/s41598-021-83949-92045-2322https://doaj.org/article/cd407f3a78dd4c5a9fc902f77a9ce81e2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83949-9https://doaj.org/toc/2045-2322Abstract The Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has turned into a pandemic with about thirty million confirmed cases worldwide as of September 2020. Being an airborne infection, it can be catastrophic to populous countries like India. This study sets to identify potential cytotoxic T lymphocyte (CTL) epitopes in the SARS-CoV-2 Indian isolate which can act as an effective vaccine epitope candidate for the majority of the Indian population. The immunogenicity and the foreignness of the epitopes towards the human body have to be studied to further confirm their candidacy. The top-scoring epitopes were subjected to molecular docking studies to study their interactions with the corresponding human leukocyte antigen (HLA) system. The CTL epitopes were observed to bind at the peptide-binding groove of the corresponding HLA system, indicating their potency as an epitope candidate. The candidacy was further analyzed using sequence conservation studies and molecular dynamics simulation. The identified epitopes can be subjected to further studies for the development of the SARS-CoV-2 vaccine.Viswajit MulpuruNidhi MishraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Viswajit Mulpuru Nidhi Mishra Immunoinformatic based identification of cytotoxic T lymphocyte epitopes from the Indian isolate of SARS-CoV-2
description	Abstract The Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has turned into a pandemic with about thirty million confirmed cases worldwide as of September 2020. Being an airborne infection, it can be catastrophic to populous countries like India. This study sets to identify potential cytotoxic T lymphocyte (CTL) epitopes in the SARS-CoV-2 Indian isolate which can act as an effective vaccine epitope candidate for the majority of the Indian population. The immunogenicity and the foreignness of the epitopes towards the human body have to be studied to further confirm their candidacy. The top-scoring epitopes were subjected to molecular docking studies to study their interactions with the corresponding human leukocyte antigen (HLA) system. The CTL epitopes were observed to bind at the peptide-binding groove of the corresponding HLA system, indicating their potency as an epitope candidate. The candidacy was further analyzed using sequence conservation studies and molecular dynamics simulation. The identified epitopes can be subjected to further studies for the development of the SARS-CoV-2 vaccine.
format	article
author	Viswajit Mulpuru Nidhi Mishra
author_facet	Viswajit Mulpuru Nidhi Mishra
author_sort	Viswajit Mulpuru
title	Immunoinformatic based identification of cytotoxic T lymphocyte epitopes from the Indian isolate of SARS-CoV-2
title_short	Immunoinformatic based identification of cytotoxic T lymphocyte epitopes from the Indian isolate of SARS-CoV-2
title_full	Immunoinformatic based identification of cytotoxic T lymphocyte epitopes from the Indian isolate of SARS-CoV-2
title_fullStr	Immunoinformatic based identification of cytotoxic T lymphocyte epitopes from the Indian isolate of SARS-CoV-2
title_full_unstemmed	Immunoinformatic based identification of cytotoxic T lymphocyte epitopes from the Indian isolate of SARS-CoV-2
title_sort	immunoinformatic based identification of cytotoxic t lymphocyte epitopes from the indian isolate of sars-cov-2
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/cd407f3a78dd4c5a9fc902f77a9ce81e
work_keys_str_mv	AT viswajitmulpuru immunoinformaticbasedidentificationofcytotoxictlymphocyteepitopesfromtheindianisolateofsarscov2 AT nidhimishra immunoinformaticbasedidentificationofcytotoxictlymphocyteepitopesfromtheindianisolateofsarscov2
_version_	1718393296424796160

Immunoinformatic based identification of cytotoxic T lymphocyte epitopes from the Indian isolate of SARS-CoV-2

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