Bacillus velezensis T149-19 and Bacillus safensis T052-76 as Potential Biocontrol Agents against Foot Rot Disease in Sweet Potato

Sweet potato (Ipomoea batatas) tuberous roots are used for human consumption, animal feed, and many industrial products. However, the crop is susceptible to various pests and diseases, including foot rot disease caused by the phytopathogenic fungus Plenodomus destruens<...

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Autores principales:	Jackeline Rossetti Mateus, Isabella Dal’Rio, Diogo Jurelevicius, Fabio Faria da Mota, Joana Montezano Marques, Rommel Thiago Juca Ramos, Artur Luiz da Costa da Silva, Paulo Roberto Gagliardi, Lucy Seldin
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Bacillus velezensis</i> <i>Bacillus safensis</i> <i>Plenodomus destruens</i> foot rot disease biocontrol sweet potato Agriculture (General) S1-972
Acceso en línea:	https://doaj.org/article/cd41825bd84c409d9e8ee49e3f84c9b7
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spelling	oai:doaj.org-article:cd41825bd84c409d9e8ee49e3f84c9b72021-11-25T15:58:12Z<i>Bacillus velezensis</i> T149-19 and <i>Bacillus safensis</i> T052-76 as Potential Biocontrol Agents against Foot Rot Disease in Sweet Potato10.3390/agriculture111110462077-0472https://doaj.org/article/cd41825bd84c409d9e8ee49e3f84c9b72021-10-01T00:00:00Zhttps://www.mdpi.com/2077-0472/11/11/1046https://doaj.org/toc/2077-0472Sweet potato (<i>Ipomoea batatas</i>) tuberous roots are used for human consumption, animal feed, and many industrial products. However, the crop is susceptible to various pests and diseases, including foot rot disease caused by the phytopathogenic fungus <i>Plenodomus destruens</i>. Biological control of plant pathogens by <i>Bacillus</i> species is widely disseminated in agrosystems, but specific biological control agents against the foot rot disease-causing fungus are not yet available. Our previous studies showed that two <i>Bacillus</i> strains isolated from sweet potato roots—<i>B. safensis</i> T052-76 and <i>B. velezensis</i> T149-19—were able to inhibit <i>P. destruens</i> in vitro, but data from in vivo experiments using simultaneously the fungus and the bacteria were missing. In this study, both strains were shown to protect the plant from the disease and to mitigate the symptoms of foot rot disease in pot experiments. Total fungal community quantification using real-time PCR showed a significant decrease in the number of copies of the ITS gene when the bacteria were inoculated, compared to the control (with the fungus only). To determine the genes encoding antimicrobial substances likely to inhibit the fungus, their genomes were sequenced and annotated. Genes coding for mycosubtilin, bacillaene, macrolactin, bacillibactin, bacilysin, plantazolicin, plipastatin, dificidine, fengycin and surfactin were found in <i>B. velezensis</i> T149-19, while those coding for bacylisin, lichenysin, bacillibactin, fengycin and surfactin were found in <i>B. safensis</i> T052-76. Altogether, the data presented here contribute to advancing the knowledge for the use of these <i>Bacillus</i> strains as biocontrol products in sweet potato.Jackeline Rossetti MateusIsabella Dal’RioDiogo JureleviciusFabio Faria da MotaJoana Montezano MarquesRommel Thiago Juca RamosArtur Luiz da Costa da SilvaPaulo Roberto GagliardiLucy SeldinMDPI AGarticle<i>Bacillus velezensis</i><i>Bacillus safensis</i><i>Plenodomus destruens</i>foot rot diseasebiocontrolsweet potatoAgriculture (General)S1-972ENAgriculture, Vol 11, Iss 1046, p 1046 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Bacillus velezensis</i> <i>Bacillus safensis</i> <i>Plenodomus destruens</i> foot rot disease biocontrol sweet potato Agriculture (General) S1-972
spellingShingle	<i>Bacillus velezensis</i> <i>Bacillus safensis</i> <i>Plenodomus destruens</i> foot rot disease biocontrol sweet potato Agriculture (General) S1-972 Jackeline Rossetti Mateus Isabella Dal’Rio Diogo Jurelevicius Fabio Faria da Mota Joana Montezano Marques Rommel Thiago Juca Ramos Artur Luiz da Costa da Silva Paulo Roberto Gagliardi Lucy Seldin <i>Bacillus velezensis</i> T149-19 and <i>Bacillus safensis</i> T052-76 as Potential Biocontrol Agents against Foot Rot Disease in Sweet Potato
description	Sweet potato (<i>Ipomoea batatas</i>) tuberous roots are used for human consumption, animal feed, and many industrial products. However, the crop is susceptible to various pests and diseases, including foot rot disease caused by the phytopathogenic fungus <i>Plenodomus destruens</i>. Biological control of plant pathogens by <i>Bacillus</i> species is widely disseminated in agrosystems, but specific biological control agents against the foot rot disease-causing fungus are not yet available. Our previous studies showed that two <i>Bacillus</i> strains isolated from sweet potato roots—<i>B. safensis</i> T052-76 and <i>B. velezensis</i> T149-19—were able to inhibit <i>P. destruens</i> in vitro, but data from in vivo experiments using simultaneously the fungus and the bacteria were missing. In this study, both strains were shown to protect the plant from the disease and to mitigate the symptoms of foot rot disease in pot experiments. Total fungal community quantification using real-time PCR showed a significant decrease in the number of copies of the ITS gene when the bacteria were inoculated, compared to the control (with the fungus only). To determine the genes encoding antimicrobial substances likely to inhibit the fungus, their genomes were sequenced and annotated. Genes coding for mycosubtilin, bacillaene, macrolactin, bacillibactin, bacilysin, plantazolicin, plipastatin, dificidine, fengycin and surfactin were found in <i>B. velezensis</i> T149-19, while those coding for bacylisin, lichenysin, bacillibactin, fengycin and surfactin were found in <i>B. safensis</i> T052-76. Altogether, the data presented here contribute to advancing the knowledge for the use of these <i>Bacillus</i> strains as biocontrol products in sweet potato.
format	article
author	Jackeline Rossetti Mateus Isabella Dal’Rio Diogo Jurelevicius Fabio Faria da Mota Joana Montezano Marques Rommel Thiago Juca Ramos Artur Luiz da Costa da Silva Paulo Roberto Gagliardi Lucy Seldin
author_facet	Jackeline Rossetti Mateus Isabella Dal’Rio Diogo Jurelevicius Fabio Faria da Mota Joana Montezano Marques Rommel Thiago Juca Ramos Artur Luiz da Costa da Silva Paulo Roberto Gagliardi Lucy Seldin
author_sort	Jackeline Rossetti Mateus
title	<i>Bacillus velezensis</i> T149-19 and <i>Bacillus safensis</i> T052-76 as Potential Biocontrol Agents against Foot Rot Disease in Sweet Potato
title_short	<i>Bacillus velezensis</i> T149-19 and <i>Bacillus safensis</i> T052-76 as Potential Biocontrol Agents against Foot Rot Disease in Sweet Potato
title_full	<i>Bacillus velezensis</i> T149-19 and <i>Bacillus safensis</i> T052-76 as Potential Biocontrol Agents against Foot Rot Disease in Sweet Potato
title_fullStr	<i>Bacillus velezensis</i> T149-19 and <i>Bacillus safensis</i> T052-76 as Potential Biocontrol Agents against Foot Rot Disease in Sweet Potato
title_full_unstemmed	<i>Bacillus velezensis</i> T149-19 and <i>Bacillus safensis</i> T052-76 as Potential Biocontrol Agents against Foot Rot Disease in Sweet Potato
title_sort	<i>bacillus velezensis</i> t149-19 and <i>bacillus safensis</i> t052-76 as potential biocontrol agents against foot rot disease in sweet potato
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/cd41825bd84c409d9e8ee49e3f84c9b7
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<i>Bacillus velezensis</i> T149-19 and <i>Bacillus safensis</i> T052-76 as Potential Biocontrol Agents against Foot Rot Disease in Sweet Potato

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