Thiourea and hydrogen peroxide priming improved K+ retention and source-sink relationship for mitigating salt stress in rice

Abstract Plant bioregulators (PBRs) represent low-cost chemicals for boosting plant defense, especially under stress conditions. In the present study, redox based PBRs such as thiourea (TU; a non-physiological thiol-based ROS scavenger) and hydrogen peroxide (H2O2; a prevalent biological ROS) were a...

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Autores principales:	Manish Pandey, Radha Krishna Paladi, Ashish Kumar Srivastava, Penna Suprasanna
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cd478b1da44140e38bf471a301b9ca81
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spelling	oai:doaj.org-article:cd478b1da44140e38bf471a301b9ca812021-12-02T14:06:57ZThiourea and hydrogen peroxide priming improved K+ retention and source-sink relationship for mitigating salt stress in rice10.1038/s41598-020-80419-62045-2322https://doaj.org/article/cd478b1da44140e38bf471a301b9ca812021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80419-6https://doaj.org/toc/2045-2322Abstract Plant bioregulators (PBRs) represent low-cost chemicals for boosting plant defense, especially under stress conditions. In the present study, redox based PBRs such as thiourea (TU; a non-physiological thiol-based ROS scavenger) and hydrogen peroxide (H2O2; a prevalent biological ROS) were assessed for their ability to mitigate NaCl stress in rice variety IR 64. Despite their contrasting redox chemistry, TU or H2O2 supplementation under NaCl [NaCl + TU (NT) or NaCl + H2O2 (NH)] generated a reducing redox environment in planta, which improved the plant growth compared with those of NaCl alone treatment. This was concomitant with better K+ retention and upregulated expression of NaCl defense related genes including HAK21, LEA1, TSPO and EN20 in both NT and NH treated seedlings. Under field conditions, foliar applications of TU and H2O2, at vegetative growth, pre-flowering and grain filling stages, increased growth and yield attributes under both control and NaCl stress conditions. Principal component analysis revealed glutathione reductase dependent reduced ROS accumulation in source (flag leaves) and sucrose synthase mediated sucrose catabolism in sink (developing inflorescence), as the key variables associated with NT and NH mediated effects, respectively. In addition, photosystem-II efficiency, K+ retention and source-sink relationship were also improved in TU and H2O2 treated plants. Taken together, our study highlights that reducing redox environment acts as a central regulator of plant’s tolerance responses to salt stress. In addition, TU and H2O2 are proposed as potential redox-based PBRs for boosting rice productivity under the realistic field conditions.Manish PandeyRadha Krishna PaladiAshish Kumar SrivastavaPenna SuprasannaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Manish Pandey Radha Krishna Paladi Ashish Kumar Srivastava Penna Suprasanna Thiourea and hydrogen peroxide priming improved K+ retention and source-sink relationship for mitigating salt stress in rice
description	Abstract Plant bioregulators (PBRs) represent low-cost chemicals for boosting plant defense, especially under stress conditions. In the present study, redox based PBRs such as thiourea (TU; a non-physiological thiol-based ROS scavenger) and hydrogen peroxide (H2O2; a prevalent biological ROS) were assessed for their ability to mitigate NaCl stress in rice variety IR 64. Despite their contrasting redox chemistry, TU or H2O2 supplementation under NaCl [NaCl + TU (NT) or NaCl + H2O2 (NH)] generated a reducing redox environment in planta, which improved the plant growth compared with those of NaCl alone treatment. This was concomitant with better K+ retention and upregulated expression of NaCl defense related genes including HAK21, LEA1, TSPO and EN20 in both NT and NH treated seedlings. Under field conditions, foliar applications of TU and H2O2, at vegetative growth, pre-flowering and grain filling stages, increased growth and yield attributes under both control and NaCl stress conditions. Principal component analysis revealed glutathione reductase dependent reduced ROS accumulation in source (flag leaves) and sucrose synthase mediated sucrose catabolism in sink (developing inflorescence), as the key variables associated with NT and NH mediated effects, respectively. In addition, photosystem-II efficiency, K+ retention and source-sink relationship were also improved in TU and H2O2 treated plants. Taken together, our study highlights that reducing redox environment acts as a central regulator of plant’s tolerance responses to salt stress. In addition, TU and H2O2 are proposed as potential redox-based PBRs for boosting rice productivity under the realistic field conditions.
format	article
author	Manish Pandey Radha Krishna Paladi Ashish Kumar Srivastava Penna Suprasanna
author_facet	Manish Pandey Radha Krishna Paladi Ashish Kumar Srivastava Penna Suprasanna
author_sort	Manish Pandey
title	Thiourea and hydrogen peroxide priming improved K+ retention and source-sink relationship for mitigating salt stress in rice
title_short	Thiourea and hydrogen peroxide priming improved K+ retention and source-sink relationship for mitigating salt stress in rice
title_full	Thiourea and hydrogen peroxide priming improved K+ retention and source-sink relationship for mitigating salt stress in rice
title_fullStr	Thiourea and hydrogen peroxide priming improved K+ retention and source-sink relationship for mitigating salt stress in rice
title_full_unstemmed	Thiourea and hydrogen peroxide priming improved K+ retention and source-sink relationship for mitigating salt stress in rice
title_sort	thiourea and hydrogen peroxide priming improved k+ retention and source-sink relationship for mitigating salt stress in rice
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/cd478b1da44140e38bf471a301b9ca81
work_keys_str_mv	AT manishpandey thioureaandhydrogenperoxideprimingimprovedkretentionandsourcesinkrelationshipformitigatingsaltstressinrice AT radhakrishnapaladi thioureaandhydrogenperoxideprimingimprovedkretentionandsourcesinkrelationshipformitigatingsaltstressinrice AT ashishkumarsrivastava thioureaandhydrogenperoxideprimingimprovedkretentionandsourcesinkrelationshipformitigatingsaltstressinrice AT pennasuprasanna thioureaandhydrogenperoxideprimingimprovedkretentionandsourcesinkrelationshipformitigatingsaltstressinrice
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Thiourea and hydrogen peroxide priming improved K+ retention and source-sink relationship for mitigating salt stress in rice

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