Novel diversity within marine Mamiellophyceae (Chlorophyta) unveiled by metabarcoding

Abstract Mamiellophyceae (unicellular green algae) are a key phytoplankton group in coastal waters. Although extensively studied over the last 20 years, the overall oceanic distribution of the major species/clades is still poorly known. To address this problem, we analyzed the 2014 Ocean Sampling Da...

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Autores principales:	Margot Tragin, Daniel Vaulot
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cd4ba837fa244e198e2757ebcc54a83c
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spelling	oai:doaj.org-article:cd4ba837fa244e198e2757ebcc54a83c2021-12-02T15:09:56ZNovel diversity within marine Mamiellophyceae (Chlorophyta) unveiled by metabarcoding10.1038/s41598-019-41680-62045-2322https://doaj.org/article/cd4ba837fa244e198e2757ebcc54a83c2019-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-41680-6https://doaj.org/toc/2045-2322Abstract Mamiellophyceae (unicellular green algae) are a key phytoplankton group in coastal waters. Although extensively studied over the last 20 years, the overall oceanic distribution of the major species/clades is still poorly known. To address this problem, we analyzed the 2014 Ocean Sampling Day (OSD) metabarcoding dataset providing sequences from the V4 hypervariable region of the 18S rRNA gene for 157 samples collected at 143 mostly coastal stations. Mamiellophyceae were found at nearly all OSD stations and represented 55% of the green microalgae (Chlorophyta) reads. We performed phylogenetic analyses of unique OSD metabarcodes (amplicon single variants, ASVs) and GenBank reference sequences from cultures and from the environment, focusing on the four most represented genera: Ostreococcus (45% of the Mamiellophyceae reads), Micromonas (34%), Bathycoccus (10%) and Mantoniella (8.7%). These analyses uncovered novel diversity within each genus except Bathycoccus. In Ostreococcus, a new clade (E) was the second most represented clade after Ostreococcus “lucimarinus”. Micromonas could be separated into nine clades, exceeding the six species and candidate species already described. Finally, we found two new environmental clades within Mantoniella. Each Mamiellophyceae clade had a specific distribution in the OSD dataset suggesting that they are adapted to different ecological niches.Margot TraginDaniel VaulotNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-14 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Margot Tragin Daniel Vaulot Novel diversity within marine Mamiellophyceae (Chlorophyta) unveiled by metabarcoding
description	Abstract Mamiellophyceae (unicellular green algae) are a key phytoplankton group in coastal waters. Although extensively studied over the last 20 years, the overall oceanic distribution of the major species/clades is still poorly known. To address this problem, we analyzed the 2014 Ocean Sampling Day (OSD) metabarcoding dataset providing sequences from the V4 hypervariable region of the 18S rRNA gene for 157 samples collected at 143 mostly coastal stations. Mamiellophyceae were found at nearly all OSD stations and represented 55% of the green microalgae (Chlorophyta) reads. We performed phylogenetic analyses of unique OSD metabarcodes (amplicon single variants, ASVs) and GenBank reference sequences from cultures and from the environment, focusing on the four most represented genera: Ostreococcus (45% of the Mamiellophyceae reads), Micromonas (34%), Bathycoccus (10%) and Mantoniella (8.7%). These analyses uncovered novel diversity within each genus except Bathycoccus. In Ostreococcus, a new clade (E) was the second most represented clade after Ostreococcus “lucimarinus”. Micromonas could be separated into nine clades, exceeding the six species and candidate species already described. Finally, we found two new environmental clades within Mantoniella. Each Mamiellophyceae clade had a specific distribution in the OSD dataset suggesting that they are adapted to different ecological niches.
format	article
author	Margot Tragin Daniel Vaulot
author_facet	Margot Tragin Daniel Vaulot
author_sort	Margot Tragin
title	Novel diversity within marine Mamiellophyceae (Chlorophyta) unveiled by metabarcoding
title_short	Novel diversity within marine Mamiellophyceae (Chlorophyta) unveiled by metabarcoding
title_full	Novel diversity within marine Mamiellophyceae (Chlorophyta) unveiled by metabarcoding
title_fullStr	Novel diversity within marine Mamiellophyceae (Chlorophyta) unveiled by metabarcoding
title_full_unstemmed	Novel diversity within marine Mamiellophyceae (Chlorophyta) unveiled by metabarcoding
title_sort	novel diversity within marine mamiellophyceae (chlorophyta) unveiled by metabarcoding
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/cd4ba837fa244e198e2757ebcc54a83c
work_keys_str_mv	AT margottragin noveldiversitywithinmarinemamiellophyceaechlorophytaunveiledbymetabarcoding AT danielvaulot noveldiversitywithinmarinemamiellophyceaechlorophytaunveiledbymetabarcoding
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Novel diversity within marine Mamiellophyceae (Chlorophyta) unveiled by metabarcoding

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