Determination of paeoniflorin and puerarin in emulsion and cosmetic water with HPLC
A high performance liquid chromatography (HPLC) method for determination of paeoniflorin and puerarin in emulsion and cosmetic water was established. The samples were extracted with methanol by ultrasonication, and separated with acetonitrile-0.1% (mass fraction) phosphoric acid aqueous solution by...
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Academic Journals Center of Shanghai Normal University
2021
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oai:doaj.org-article:cd515abf391c450fa246117fe5e959952021-11-12T03:34:00ZDetermination of paeoniflorin and puerarin in emulsion and cosmetic water with HPLC1000-513710.3969/J.ISSN.1000-5137.2021.05.013https://doaj.org/article/cd515abf391c450fa246117fe5e959952021-10-01T00:00:00Zhttp://qktg.shnu.edu.cn/zrb/shsfqkszrb/ch/reader/view_abstract.aspx?file_no=20210513https://doaj.org/toc/1000-5137A high performance liquid chromatography (HPLC) method for determination of paeoniflorin and puerarin in emulsion and cosmetic water was established. The samples were extracted with methanol by ultrasonication, and separated with acetonitrile-0.1% (mass fraction) phosphoric acid aqueous solution by gradient elution using C<sub>18</sub> RP column and then inspected by diode array detector (DAD) detector. Under the optimum conditions,The linear relationship of the concentrations of paeoniflorin and puerarin and the intensity of UV absorptions at 232 nm and 250 nm was good in a range of 0.2-10.0 μg·mL<sup>-1</sup>,and the squared correlation coefficient (<i>R</i><sup>2</sup>) was greater than 0.999. The recovery rate of two herbs was between 90.4% and 103.9%, and the relative standard deviation (RSD) was between 1.53% and 2.37%. The detection limit was between 2 mg·kg<sup>-1</sup> and 8 mg·kg<sup>-1</sup>, meeting the requirements of the national standard for the detection limit. The developed method is suitable for the rapid, efficient, and accurate determination of paeoniflorin and puerarin in emulsion and cosmetic water.HU XiaorongWANG YizeLOU ZhiyingHU GangZHOU GuoguangAcademic Journals Center of Shanghai Normal Universityarticlehigh performance liquid chromatography(hplc)paeoniflorinpuerarinemulsioncosmetic waterScience (General)Q1-390ENZHJournal of Shanghai Normal University (Natural Sciences), Vol 50, Iss 5, Pp 623-628 (2021) |
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DOAJ |
| language |
EN ZH |
| topic |
high performance liquid chromatography(hplc) paeoniflorin puerarin emulsion cosmetic water Science (General) Q1-390 |
| spellingShingle |
high performance liquid chromatography(hplc) paeoniflorin puerarin emulsion cosmetic water Science (General) Q1-390 HU Xiaorong WANG Yize LOU Zhiying HU Gang ZHOU Guoguang Determination of paeoniflorin and puerarin in emulsion and cosmetic water with HPLC |
| description |
A high performance liquid chromatography (HPLC) method for determination of paeoniflorin and puerarin in emulsion and cosmetic water was established. The samples were extracted with methanol by ultrasonication, and separated with acetonitrile-0.1% (mass fraction) phosphoric acid aqueous solution by gradient elution using C<sub>18</sub> RP column and then inspected by diode array detector (DAD) detector. Under the optimum conditions,The linear relationship of the concentrations of paeoniflorin and puerarin and the intensity of UV absorptions at 232 nm and 250 nm was good in a range of 0.2-10.0 μg·mL<sup>-1</sup>,and the squared correlation coefficient (<i>R</i><sup>2</sup>) was greater than 0.999. The recovery rate of two herbs was between 90.4% and 103.9%, and the relative standard deviation (RSD) was between 1.53% and 2.37%. The detection limit was between 2 mg·kg<sup>-1</sup> and 8 mg·kg<sup>-1</sup>, meeting the requirements of the national standard for the detection limit. The developed method is suitable for the rapid, efficient, and accurate determination of paeoniflorin and puerarin in emulsion and cosmetic water. |
| format |
article |
| author |
HU Xiaorong WANG Yize LOU Zhiying HU Gang ZHOU Guoguang |
| author_facet |
HU Xiaorong WANG Yize LOU Zhiying HU Gang ZHOU Guoguang |
| author_sort |
HU Xiaorong |
| title |
Determination of paeoniflorin and puerarin in emulsion and cosmetic water with HPLC |
| title_short |
Determination of paeoniflorin and puerarin in emulsion and cosmetic water with HPLC |
| title_full |
Determination of paeoniflorin and puerarin in emulsion and cosmetic water with HPLC |
| title_fullStr |
Determination of paeoniflorin and puerarin in emulsion and cosmetic water with HPLC |
| title_full_unstemmed |
Determination of paeoniflorin and puerarin in emulsion and cosmetic water with HPLC |
| title_sort |
determination of paeoniflorin and puerarin in emulsion and cosmetic water with hplc |
| publisher |
Academic Journals Center of Shanghai Normal University |
| publishDate |
2021 |
| url |
https://doaj.org/article/cd515abf391c450fa246117fe5e95995 |
| work_keys_str_mv |
AT huxiaorong determinationofpaeoniflorinandpuerarininemulsionandcosmeticwaterwithhplc AT wangyize determinationofpaeoniflorinandpuerarininemulsionandcosmeticwaterwithhplc AT louzhiying determinationofpaeoniflorinandpuerarininemulsionandcosmeticwaterwithhplc AT hugang determinationofpaeoniflorinandpuerarininemulsionandcosmeticwaterwithhplc AT zhouguoguang determinationofpaeoniflorinandpuerarininemulsionandcosmeticwaterwithhplc |
| _version_ |
1718431316546945024 |