Desensitized D2 autoreceptors are resistant to trafficking

Abstract Dendritic release of dopamine activates dopamine D2 autoreceptors, which are inhibitory G protein-coupled receptors (GPCRs), to decrease the excitability of dopamine neurons. This study used tagged D2 receptors to identify the localization and distribution of these receptors in living midbr...

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Autores principales:	Brooks G. Robinson, James R. Bunzow, Jonathan B. Grimm, Luke D. Lavis, Joshua T. Dudman, Jennifer Brown, Kim A. Neve, John T. Williams
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cd52b855e0284c209df9fce0fd4cbae8
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spelling	oai:doaj.org-article:cd52b855e0284c209df9fce0fd4cbae82021-12-02T11:40:58ZDesensitized D2 autoreceptors are resistant to trafficking10.1038/s41598-017-04728-z2045-2322https://doaj.org/article/cd52b855e0284c209df9fce0fd4cbae82017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04728-zhttps://doaj.org/toc/2045-2322Abstract Dendritic release of dopamine activates dopamine D2 autoreceptors, which are inhibitory G protein-coupled receptors (GPCRs), to decrease the excitability of dopamine neurons. This study used tagged D2 receptors to identify the localization and distribution of these receptors in living midbrain dopamine neurons. GFP-tagged D2 receptors were found to be unevenly clustered on the soma and dendrites of dopamine neurons within the substantia nigra pars compacta (SNc). Physiological signaling and desensitization of the tagged receptors were not different from wild type receptors. Unexpectedly, upon desensitization the tagged D2 receptors were not internalized. When tagged D2 receptors were expressed in locus coeruleus neurons, a desensitizing protocol induced significant internalization. Likewise, when tagged µ-opioid receptors were expressed in dopamine neurons they too were internalized. The distribution and lack of agonist-induced internalization of D2 receptors on dopamine neurons indicate a purposefully regulated localization of these receptors.Brooks G. RobinsonJames R. BunzowJonathan B. GrimmLuke D. LavisJoshua T. DudmanJennifer BrownKim A. NeveJohn T. WilliamsNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Brooks G. Robinson James R. Bunzow Jonathan B. Grimm Luke D. Lavis Joshua T. Dudman Jennifer Brown Kim A. Neve John T. Williams Desensitized D2 autoreceptors are resistant to trafficking
description	Abstract Dendritic release of dopamine activates dopamine D2 autoreceptors, which are inhibitory G protein-coupled receptors (GPCRs), to decrease the excitability of dopamine neurons. This study used tagged D2 receptors to identify the localization and distribution of these receptors in living midbrain dopamine neurons. GFP-tagged D2 receptors were found to be unevenly clustered on the soma and dendrites of dopamine neurons within the substantia nigra pars compacta (SNc). Physiological signaling and desensitization of the tagged receptors were not different from wild type receptors. Unexpectedly, upon desensitization the tagged D2 receptors were not internalized. When tagged D2 receptors were expressed in locus coeruleus neurons, a desensitizing protocol induced significant internalization. Likewise, when tagged µ-opioid receptors were expressed in dopamine neurons they too were internalized. The distribution and lack of agonist-induced internalization of D2 receptors on dopamine neurons indicate a purposefully regulated localization of these receptors.
format	article
author	Brooks G. Robinson James R. Bunzow Jonathan B. Grimm Luke D. Lavis Joshua T. Dudman Jennifer Brown Kim A. Neve John T. Williams
author_facet	Brooks G. Robinson James R. Bunzow Jonathan B. Grimm Luke D. Lavis Joshua T. Dudman Jennifer Brown Kim A. Neve John T. Williams
author_sort	Brooks G. Robinson
title	Desensitized D2 autoreceptors are resistant to trafficking
title_short	Desensitized D2 autoreceptors are resistant to trafficking
title_full	Desensitized D2 autoreceptors are resistant to trafficking
title_fullStr	Desensitized D2 autoreceptors are resistant to trafficking
title_full_unstemmed	Desensitized D2 autoreceptors are resistant to trafficking
title_sort	desensitized d2 autoreceptors are resistant to trafficking
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/cd52b855e0284c209df9fce0fd4cbae8
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Desensitized D2 autoreceptors are resistant to trafficking

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