The Interplay of Autoclaving with Oxalate as Pretreatment Technique in the View of Bioethanol Production Based on Corn Stover

Bio-based treatment technologies are gaining great interest worldwide, and significant efforts are being afforded to develop technology for the use of lignocellulosic biomass. The potential of corn stover (CS) as a feedstock for bioethanol production was investigated by creating an optimal pretreatm...

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Autores principales:	Ali Hamdy, Sara Abd Elhafez, Hesham Hamad, Rehab Ali
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	delignification lignocellulose ultrasonic irradiation autoclaving response surface methodology enzymatic hydrolysis Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/cd5c5a9d135d4557a01c2250cf3239f7
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spelling	oai:doaj.org-article:cd5c5a9d135d4557a01c2250cf3239f72021-11-11T18:46:39ZThe Interplay of Autoclaving with Oxalate as Pretreatment Technique in the View of Bioethanol Production Based on Corn Stover10.3390/polym132137622073-4360https://doaj.org/article/cd5c5a9d135d4557a01c2250cf3239f72021-10-01T00:00:00Zhttps://www.mdpi.com/2073-4360/13/21/3762https://doaj.org/toc/2073-4360Bio-based treatment technologies are gaining great interest worldwide, and significant efforts are being afforded to develop technology for the use of lignocellulosic biomass. The potential of corn stover (CS) as a feedstock for bioethanol production was investigated by creating an optimal pretreatment condition to maximize glucose production. The current study undertook the impact of novel physico-chemical pretreatment methods of CS, i.e., autoclave-assisted oxalate (CSOA) and ultrasound-assisted oxalate (CSOU), on the chemical composition of CS and subsequent saccharification and fermentation for bioethanol production. The delignification was monitored by physicochemical characterizations such as SEM, XRD, FTIR, CHNs, and TGA. The results evidenced that delignification and enzymatic saccharification of the CS pretreated by CSOA was higher than CSOU. The optimum enzymatic saccharification operating conditions were 1:30 g solid substrate/mL sodium acetate buffer at 50 °C, shaking speed 100 rpm, and 0.4 g enzyme dosage. This condition was applied to produce glucose from CS, followed by bioethanol production by <i>S. cerevisiae</i> using an anaerobic fermentation process after 72 h. <i>S. cerevisiae</i> showed high conversion efficiency by producing a 360 mg/dL bioethanol yield, which is considered 94.11% of the theoretical ethanol yield. Furthermore, this research provides a potential path for waste material beneficiation, such as through utilizing CS.Ali HamdySara Abd ElhafezHesham HamadRehab AliMDPI AGarticledelignificationlignocelluloseultrasonic irradiationautoclavingresponse surface methodologyenzymatic hydrolysisOrganic chemistryQD241-441ENPolymers, Vol 13, Iss 3762, p 3762 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	delignification lignocellulose ultrasonic irradiation autoclaving response surface methodology enzymatic hydrolysis Organic chemistry QD241-441
spellingShingle	delignification lignocellulose ultrasonic irradiation autoclaving response surface methodology enzymatic hydrolysis Organic chemistry QD241-441 Ali Hamdy Sara Abd Elhafez Hesham Hamad Rehab Ali The Interplay of Autoclaving with Oxalate as Pretreatment Technique in the View of Bioethanol Production Based on Corn Stover
description	Bio-based treatment technologies are gaining great interest worldwide, and significant efforts are being afforded to develop technology for the use of lignocellulosic biomass. The potential of corn stover (CS) as a feedstock for bioethanol production was investigated by creating an optimal pretreatment condition to maximize glucose production. The current study undertook the impact of novel physico-chemical pretreatment methods of CS, i.e., autoclave-assisted oxalate (CSOA) and ultrasound-assisted oxalate (CSOU), on the chemical composition of CS and subsequent saccharification and fermentation for bioethanol production. The delignification was monitored by physicochemical characterizations such as SEM, XRD, FTIR, CHNs, and TGA. The results evidenced that delignification and enzymatic saccharification of the CS pretreated by CSOA was higher than CSOU. The optimum enzymatic saccharification operating conditions were 1:30 g solid substrate/mL sodium acetate buffer at 50 °C, shaking speed 100 rpm, and 0.4 g enzyme dosage. This condition was applied to produce glucose from CS, followed by bioethanol production by <i>S. cerevisiae</i> using an anaerobic fermentation process after 72 h. <i>S. cerevisiae</i> showed high conversion efficiency by producing a 360 mg/dL bioethanol yield, which is considered 94.11% of the theoretical ethanol yield. Furthermore, this research provides a potential path for waste material beneficiation, such as through utilizing CS.
format	article
author	Ali Hamdy Sara Abd Elhafez Hesham Hamad Rehab Ali
author_facet	Ali Hamdy Sara Abd Elhafez Hesham Hamad Rehab Ali
author_sort	Ali Hamdy
title	The Interplay of Autoclaving with Oxalate as Pretreatment Technique in the View of Bioethanol Production Based on Corn Stover
title_short	The Interplay of Autoclaving with Oxalate as Pretreatment Technique in the View of Bioethanol Production Based on Corn Stover
title_full	The Interplay of Autoclaving with Oxalate as Pretreatment Technique in the View of Bioethanol Production Based on Corn Stover
title_fullStr	The Interplay of Autoclaving with Oxalate as Pretreatment Technique in the View of Bioethanol Production Based on Corn Stover
title_full_unstemmed	The Interplay of Autoclaving with Oxalate as Pretreatment Technique in the View of Bioethanol Production Based on Corn Stover
title_sort	interplay of autoclaving with oxalate as pretreatment technique in the view of bioethanol production based on corn stover
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/cd5c5a9d135d4557a01c2250cf3239f7
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The Interplay of Autoclaving with Oxalate as Pretreatment Technique in the View of Bioethanol Production Based on Corn Stover

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