Improved Biotransformation of Platycoside E into Deapiose-Xylosylated Platycodin D by Cytolase PCL5 under High Hydrostatic Pressure

Platycosides are the functional saponins present in balloon flowers that exert diverse biological effects, and which can be further improved by their deglycosylation. Deapiose-xylosylated platycodin D, which is absent in balloon flowers, can be generated only by cytolase PCL5 by acting on platycosid...

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Autores principales:	Kyung-Chul Shin, Min-Ju Seo, Yu Jin Oh, Dae Wook Kim, Chae Sun Na, Yeong-Su Kim
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	platycoside cytolase PCL5 balloon flower deglycosylation high hydrostatic pressure Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/cd5e57bcbcc54f9daf4c2b3352e4f3e8
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spelling	oai:doaj.org-article:cd5e57bcbcc54f9daf4c2b3352e4f3e82021-11-25T16:33:32ZImproved Biotransformation of Platycoside E into Deapiose-Xylosylated Platycodin D by Cytolase PCL5 under High Hydrostatic Pressure10.3390/app1122106232076-3417https://doaj.org/article/cd5e57bcbcc54f9daf4c2b3352e4f3e82021-11-01T00:00:00Zhttps://www.mdpi.com/2076-3417/11/22/10623https://doaj.org/toc/2076-3417Platycosides are the functional saponins present in balloon flowers that exert diverse biological effects, and which can be further improved by their deglycosylation. Deapiose-xylosylated platycodin D, which is absent in balloon flowers, can be generated only by cytolase PCL5 by acting on platycoside E. To improve cytolase PCL5-catalyzed production of deapiose-xylosylated platycodin D from platycoside E, we explored the use of high hydrostatic pressure (HHP). At an HHP of 150 MPa, the optimal temperature of cytolase PCL5 activity for converting platycoside E into deapiose-xylosylated platycodin D shifted from 50 to 55 °C, and increased the activity and stability of the enzyme by 5- and 4.9-fold, respectively. Under HHP, the enzyme completely converted 1 mM platycoside E into deapiose-xylosylated platycodin D within 4 h, with a 3.75-fold higher productivity than that under atmospheric pressure. Our results suggest that the application of HHP is a potential method for the economical production of platycosides and enzyme-catalyzed biotransformation of functional saponins.Kyung-Chul ShinMin-Ju SeoYu Jin OhDae Wook KimChae Sun NaYeong-Su KimMDPI AGarticleplatycosidecytolase PCL5balloon flowerdeglycosylationhigh hydrostatic pressureTechnologyTEngineering (General). Civil engineering (General)TA1-2040Biology (General)QH301-705.5PhysicsQC1-999ChemistryQD1-999ENApplied Sciences, Vol 11, Iss 10623, p 10623 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	platycoside cytolase PCL5 balloon flower deglycosylation high hydrostatic pressure Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999
spellingShingle	platycoside cytolase PCL5 balloon flower deglycosylation high hydrostatic pressure Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999 Kyung-Chul Shin Min-Ju Seo Yu Jin Oh Dae Wook Kim Chae Sun Na Yeong-Su Kim Improved Biotransformation of Platycoside E into Deapiose-Xylosylated Platycodin D by Cytolase PCL5 under High Hydrostatic Pressure
description	Platycosides are the functional saponins present in balloon flowers that exert diverse biological effects, and which can be further improved by their deglycosylation. Deapiose-xylosylated platycodin D, which is absent in balloon flowers, can be generated only by cytolase PCL5 by acting on platycoside E. To improve cytolase PCL5-catalyzed production of deapiose-xylosylated platycodin D from platycoside E, we explored the use of high hydrostatic pressure (HHP). At an HHP of 150 MPa, the optimal temperature of cytolase PCL5 activity for converting platycoside E into deapiose-xylosylated platycodin D shifted from 50 to 55 °C, and increased the activity and stability of the enzyme by 5- and 4.9-fold, respectively. Under HHP, the enzyme completely converted 1 mM platycoside E into deapiose-xylosylated platycodin D within 4 h, with a 3.75-fold higher productivity than that under atmospheric pressure. Our results suggest that the application of HHP is a potential method for the economical production of platycosides and enzyme-catalyzed biotransformation of functional saponins.
format	article
author	Kyung-Chul Shin Min-Ju Seo Yu Jin Oh Dae Wook Kim Chae Sun Na Yeong-Su Kim
author_facet	Kyung-Chul Shin Min-Ju Seo Yu Jin Oh Dae Wook Kim Chae Sun Na Yeong-Su Kim
author_sort	Kyung-Chul Shin
title	Improved Biotransformation of Platycoside E into Deapiose-Xylosylated Platycodin D by Cytolase PCL5 under High Hydrostatic Pressure
title_short	Improved Biotransformation of Platycoside E into Deapiose-Xylosylated Platycodin D by Cytolase PCL5 under High Hydrostatic Pressure
title_full	Improved Biotransformation of Platycoside E into Deapiose-Xylosylated Platycodin D by Cytolase PCL5 under High Hydrostatic Pressure
title_fullStr	Improved Biotransformation of Platycoside E into Deapiose-Xylosylated Platycodin D by Cytolase PCL5 under High Hydrostatic Pressure
title_full_unstemmed	Improved Biotransformation of Platycoside E into Deapiose-Xylosylated Platycodin D by Cytolase PCL5 under High Hydrostatic Pressure
title_sort	improved biotransformation of platycoside e into deapiose-xylosylated platycodin d by cytolase pcl5 under high hydrostatic pressure
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/cd5e57bcbcc54f9daf4c2b3352e4f3e8
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_version_	1718413167253520384

Improved Biotransformation of Platycoside E into Deapiose-Xylosylated Platycodin D by Cytolase PCL5 under High Hydrostatic Pressure

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