Activation of matrix metalloproteinases and FoxO3a in HaCaT keratinocytes by radiofrequency electromagnetic field exposure

Abstract As the skin is the largest body organ and critically serves as a barrier, it is frequently exposed and could be physiologically affected by radiofrequency electromagnetic field (RF-EMF) exposure. In this study, we found that 1760 MHz RF-EMF (4.0 W/kg specific absorption rate for 2 h/day dur...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ju Hwan Kim, Dong-Jun Kang, Jun-Sang Bae, Jai Hyuen Lee, Sangbong Jeon, Hyung-Do Choi, Nam Kim, Hyung-Gun Kim, Hak Rim Kim
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cd61121e2bb24059ae0227d2b5292b9f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:cd61121e2bb24059ae0227d2b5292b9f
record_format	dspace
spelling	oai:doaj.org-article:cd61121e2bb24059ae0227d2b5292b9f2021-12-02T14:17:19ZActivation of matrix metalloproteinases and FoxO3a in HaCaT keratinocytes by radiofrequency electromagnetic field exposure10.1038/s41598-021-87263-22045-2322https://doaj.org/article/cd61121e2bb24059ae0227d2b5292b9f2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87263-2https://doaj.org/toc/2045-2322Abstract As the skin is the largest body organ and critically serves as a barrier, it is frequently exposed and could be physiologically affected by radiofrequency electromagnetic field (RF-EMF) exposure. In this study, we found that 1760 MHz RF-EMF (4.0 W/kg specific absorption rate for 2 h/day during 4 days) exposure could induce intracellular reactive oxygen species (ROS) production in HaCaT human keratinocytes using 2′,7′-dichlorofluorescin diacetate fluorescent probe analysis. However, cell growth and viability were unaffected by RF-EMF exposure. Since oxidative stress in the skin greatly influences the skin-aging process, we analyzed the skin senescence-related factors activated by ROS generation. Matrix metalloproteinases 1, 3, and 7 (MMP1, MMP3, and MMP7), the main skin wrinkle-related proteins, were significantly increased in HaCaT cells after RF-EMF exposure. Additionally, the gelatinolytic activities of secreted MMP2 and MMP9 were also increased by RF-EMF exposure. FoxO3a (Ser318/321) and ERK1/2 (Thr 202/Tyr 204) phosphorylation levels were significantly increased by RF-EMF exposure. However, Bcl2 and Bax expression levels were not significantly changed, indicating that the apoptotic pathway was not activated in keratinocytes following RF-EMF exposure. In summary, our findings show that exposure to 1760 MHz RF-EMF induces ROS generation, leading to MMP activation and FoxO3a and ERK1/2 phosphorylation. These data suggest that RF-EMF exposure induces cellular senescence of skin cells through ROS induction in HaCaT human keratinocytes.Ju Hwan KimDong-Jun KangJun-Sang BaeJai Hyuen LeeSangbong JeonHyung-Do ChoiNam KimHyung-Gun KimHak Rim KimNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ju Hwan Kim Dong-Jun Kang Jun-Sang Bae Jai Hyuen Lee Sangbong Jeon Hyung-Do Choi Nam Kim Hyung-Gun Kim Hak Rim Kim Activation of matrix metalloproteinases and FoxO3a in HaCaT keratinocytes by radiofrequency electromagnetic field exposure
description	Abstract As the skin is the largest body organ and critically serves as a barrier, it is frequently exposed and could be physiologically affected by radiofrequency electromagnetic field (RF-EMF) exposure. In this study, we found that 1760 MHz RF-EMF (4.0 W/kg specific absorption rate for 2 h/day during 4 days) exposure could induce intracellular reactive oxygen species (ROS) production in HaCaT human keratinocytes using 2′,7′-dichlorofluorescin diacetate fluorescent probe analysis. However, cell growth and viability were unaffected by RF-EMF exposure. Since oxidative stress in the skin greatly influences the skin-aging process, we analyzed the skin senescence-related factors activated by ROS generation. Matrix metalloproteinases 1, 3, and 7 (MMP1, MMP3, and MMP7), the main skin wrinkle-related proteins, were significantly increased in HaCaT cells after RF-EMF exposure. Additionally, the gelatinolytic activities of secreted MMP2 and MMP9 were also increased by RF-EMF exposure. FoxO3a (Ser318/321) and ERK1/2 (Thr 202/Tyr 204) phosphorylation levels were significantly increased by RF-EMF exposure. However, Bcl2 and Bax expression levels were not significantly changed, indicating that the apoptotic pathway was not activated in keratinocytes following RF-EMF exposure. In summary, our findings show that exposure to 1760 MHz RF-EMF induces ROS generation, leading to MMP activation and FoxO3a and ERK1/2 phosphorylation. These data suggest that RF-EMF exposure induces cellular senescence of skin cells through ROS induction in HaCaT human keratinocytes.
format	article
author	Ju Hwan Kim Dong-Jun Kang Jun-Sang Bae Jai Hyuen Lee Sangbong Jeon Hyung-Do Choi Nam Kim Hyung-Gun Kim Hak Rim Kim
author_facet	Ju Hwan Kim Dong-Jun Kang Jun-Sang Bae Jai Hyuen Lee Sangbong Jeon Hyung-Do Choi Nam Kim Hyung-Gun Kim Hak Rim Kim
author_sort	Ju Hwan Kim
title	Activation of matrix metalloproteinases and FoxO3a in HaCaT keratinocytes by radiofrequency electromagnetic field exposure
title_short	Activation of matrix metalloproteinases and FoxO3a in HaCaT keratinocytes by radiofrequency electromagnetic field exposure
title_full	Activation of matrix metalloproteinases and FoxO3a in HaCaT keratinocytes by radiofrequency electromagnetic field exposure
title_fullStr	Activation of matrix metalloproteinases and FoxO3a in HaCaT keratinocytes by radiofrequency electromagnetic field exposure
title_full_unstemmed	Activation of matrix metalloproteinases and FoxO3a in HaCaT keratinocytes by radiofrequency electromagnetic field exposure
title_sort	activation of matrix metalloproteinases and foxo3a in hacat keratinocytes by radiofrequency electromagnetic field exposure
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/cd61121e2bb24059ae0227d2b5292b9f
work_keys_str_mv	AT juhwankim activationofmatrixmetalloproteinasesandfoxo3ainhacatkeratinocytesbyradiofrequencyelectromagneticfieldexposure AT dongjunkang activationofmatrixmetalloproteinasesandfoxo3ainhacatkeratinocytesbyradiofrequencyelectromagneticfieldexposure AT junsangbae activationofmatrixmetalloproteinasesandfoxo3ainhacatkeratinocytesbyradiofrequencyelectromagneticfieldexposure AT jaihyuenlee activationofmatrixmetalloproteinasesandfoxo3ainhacatkeratinocytesbyradiofrequencyelectromagneticfieldexposure AT sangbongjeon activationofmatrixmetalloproteinasesandfoxo3ainhacatkeratinocytesbyradiofrequencyelectromagneticfieldexposure AT hyungdochoi activationofmatrixmetalloproteinasesandfoxo3ainhacatkeratinocytesbyradiofrequencyelectromagneticfieldexposure AT namkim activationofmatrixmetalloproteinasesandfoxo3ainhacatkeratinocytesbyradiofrequencyelectromagneticfieldexposure AT hyunggunkim activationofmatrixmetalloproteinasesandfoxo3ainhacatkeratinocytesbyradiofrequencyelectromagneticfieldexposure AT hakrimkim activationofmatrixmetalloproteinasesandfoxo3ainhacatkeratinocytesbyradiofrequencyelectromagneticfieldexposure
_version_	1718391634565005312

Activation of matrix metalloproteinases and FoxO3a in HaCaT keratinocytes by radiofrequency electromagnetic field exposure

Ejemplares similares