The endocytic pathway taken by cationic substances requires Rab14 but not Rab5 and Rab7

Summary: Endocytosis and endosome dynamics are controlled by proteins of the small GTPase Rab family. Besides possible recycling routes to the plasma membrane and various organelles, previously described endocytic pathways (e.g., clathrin-mediated endocytosis, macropinocytosis, CLIC/GEEC pathway) al...

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Autores principales:	Evgeniya Trofimenko, Yuta Homma, Mitsunori Fukuda, Christian Widmann
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	endocytosis endosomes Rab5 Rab7 Rab14 cell-penetrating peptides Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/cd6588bc1b7e41f99d2228f375181f80
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spelling	oai:doaj.org-article:cd6588bc1b7e41f99d2228f375181f802021-11-04T04:29:41ZThe endocytic pathway taken by cationic substances requires Rab14 but not Rab5 and Rab72211-124710.1016/j.celrep.2021.109945https://doaj.org/article/cd6588bc1b7e41f99d2228f375181f802021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2211124721014182https://doaj.org/toc/2211-1247Summary: Endocytosis and endosome dynamics are controlled by proteins of the small GTPase Rab family. Besides possible recycling routes to the plasma membrane and various organelles, previously described endocytic pathways (e.g., clathrin-mediated endocytosis, macropinocytosis, CLIC/GEEC pathway) all appear to funnel the endocytosed material to Rab5-positive early endosomes that then mature into Rab7-positive late endosomes/lysosomes. By studying the uptake of a series of cell-penetrating peptides (CPPs), we identify an endocytic pathway that moves material to nonacidic Lamp1-positive late endosomes. Trafficking via this endocytic route is fully independent of Rab5 and Rab7 but requires the Rab14 protein. The pathway taken by CPPs differs from the conventional Rab5-dependent endocytosis at the stage of vesicle formation already, as it is not affected by a series of compounds that inhibit macropinocytosis or clathrin-mediated endocytosis. The Rab14-dependent pathway is also used by physiological cationic molecules such as polyamines and homeodomains found in homeoproteins.Evgeniya TrofimenkoYuta HommaMitsunori FukudaChristian WidmannElsevierarticleendocytosisendosomesRab5Rab7Rab14cell-penetrating peptidesBiology (General)QH301-705.5ENCell Reports, Vol 37, Iss 5, Pp 109945- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	endocytosis endosomes Rab5 Rab7 Rab14 cell-penetrating peptides Biology (General) QH301-705.5
spellingShingle	endocytosis endosomes Rab5 Rab7 Rab14 cell-penetrating peptides Biology (General) QH301-705.5 Evgeniya Trofimenko Yuta Homma Mitsunori Fukuda Christian Widmann The endocytic pathway taken by cationic substances requires Rab14 but not Rab5 and Rab7
description	Summary: Endocytosis and endosome dynamics are controlled by proteins of the small GTPase Rab family. Besides possible recycling routes to the plasma membrane and various organelles, previously described endocytic pathways (e.g., clathrin-mediated endocytosis, macropinocytosis, CLIC/GEEC pathway) all appear to funnel the endocytosed material to Rab5-positive early endosomes that then mature into Rab7-positive late endosomes/lysosomes. By studying the uptake of a series of cell-penetrating peptides (CPPs), we identify an endocytic pathway that moves material to nonacidic Lamp1-positive late endosomes. Trafficking via this endocytic route is fully independent of Rab5 and Rab7 but requires the Rab14 protein. The pathway taken by CPPs differs from the conventional Rab5-dependent endocytosis at the stage of vesicle formation already, as it is not affected by a series of compounds that inhibit macropinocytosis or clathrin-mediated endocytosis. The Rab14-dependent pathway is also used by physiological cationic molecules such as polyamines and homeodomains found in homeoproteins.
format	article
author	Evgeniya Trofimenko Yuta Homma Mitsunori Fukuda Christian Widmann
author_facet	Evgeniya Trofimenko Yuta Homma Mitsunori Fukuda Christian Widmann
author_sort	Evgeniya Trofimenko
title	The endocytic pathway taken by cationic substances requires Rab14 but not Rab5 and Rab7
title_short	The endocytic pathway taken by cationic substances requires Rab14 but not Rab5 and Rab7
title_full	The endocytic pathway taken by cationic substances requires Rab14 but not Rab5 and Rab7
title_fullStr	The endocytic pathway taken by cationic substances requires Rab14 but not Rab5 and Rab7
title_full_unstemmed	The endocytic pathway taken by cationic substances requires Rab14 but not Rab5 and Rab7
title_sort	endocytic pathway taken by cationic substances requires rab14 but not rab5 and rab7
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/cd6588bc1b7e41f99d2228f375181f80
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The endocytic pathway taken by cationic substances requires Rab14 but not Rab5 and Rab7

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